A5035678472- Fungal and yeast genetics research
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- RNA Research and Splicing
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- DNA and Nucleic Acid Chemistry
- Microtubule and mitosis dynamics
- Bacterial Genetics and Biotechnology
- Ubiquitin and proteasome pathways
- Chromosomal and Genetic Variations
- Herpesvirus Infections and Treatments
- RNA modifications and cancer
- Cytomegalovirus and herpesvirus research
- Viral-associated cancers and disorders
- Plant Reproductive Biology
- RNA regulation and disease
- Genomic variations and chromosomal abnormalities
- Plant nutrient uptake and metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Genetic and Mutation Studies
- Fungal Biology and Applications
- Environmental Monitoring and Data Management
- Monoclonal and Polyclonal Antibodies Research
University of Virginia
1989-2018
University of Virginia Health System
2007-2008
University at Albany, State University of New York
2003
New York State Department of Health
2003
Wadsworth Center
2003
University of Virginia Cancer Center
1997-2002
Johns Hopkins University
1972-1997
The University of Texas MD Anderson Cancer Center
1996
University of British Columbia
1982
Repression of yeast a cell-specific genes by the global repressor Ssn6/Tup1 has been linked to specific organization chromatin. We report here that Tup1 directly interacts with amino-terminal tails histones H3 and H4, providing molecular basis for this connection. This interaction appears be required function because mutations in H4 weaken interactions cause derepression both DNA damage-inducible genes. Moreover, histone-binding domain coincides previously defined repression domain....
The nucleosome is the fundamental unit of assembly chromosome and reversible modifications histones have been suggested to be important in many aspects function. structure-function relations amino-terminal domain yeast histone H4 were examined by creation directed point mutations. four lysines subject acetylation essential for function as substitution arginine or asparagine at these positions was lethal. No single lysine residue completely since substitutions each position viable, although...
An autonomously replicating segment, ARS, is located 293 base pairs downstream from the histone H4 gene at copy-I H3-H4 locus. The sequences needed for autonomous replication were defined by deletion analysis to include an ARS consensus sequence and additional 3'-flanking region. External deletions into yeast resulted in a loss of function. However, disruptions required domain either 10-base-pair linker-scanning substitutions or larger internal did not impair replication. Thus, dependent...
Yaf9 is one of three proteins in budding yeast containing a YEATS domain. We show that part large complex and it coprecipitates with known subunits the NuA4 histone acetyltransferase. Although Esa1, catalytic subunit NuA4, essential for viability, we found yaf9Δ mutants are viable but hypersensitive to microtubule depolymerizing agents synthetically lethal two different mitotic apparatus. Microtubules depolymerized more readily mutant compared wild type presence nocodazole, recovery...
The N-terminal domains of the histones H3 and H4 are highly conserved throughout evolution. Mutant alleles deleted for these were constructed in vitro examined function vivo Saccharomyces cerevisiae. Cells containing a single deletion allele either histone or viable. Deletion domain caused cells to become sterile temperature sensitive growth. normal cell cycle progression was also altered, as revealed by major delay through G2 + M periods. had only minor effects on mating...
Messenger RNA sequences for immunoglobulin kappa light chain were synthesized in vitro isolated mouse myeloma nuclei using bound endogenous polymerase (RNA nucleotidyltransferase; nucleoside triphosphate:RNA EC 2.7.7.6) and from chromatin exogenous Escherichia coli polymerase. The was transcribed 5-mercuriuridine triphosphate separated vivo by chromatography on an agarose sulfhydryl affinity column. Template restriction is retained since synthesis of messenger RNA, As determined...
The haploid genome of Saccharomyces cerevisiae contains two nonallelic sets histone H3 and H4 gene pairs, termed the copy I II loci. structures mRNA transcripts from each these four genes were examined by nuclease protection primer extension mapping. For gene, several species mRNAs identified that differed in lengths their 5' 3' untranslated regions. cell cycle accumulation pattern was determined cells early-exponential-growth cultures fractionated centrifugal elutriation. RNA all regulated...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIncorporation of purine nucleoside 5'-[γ-S]triphosphates as affinity probes for initiation RNA synthesis in vitroAnthony E. Reeve, M. Mitchell Smith, Vincent Pigiet, and Ru Chih C. HuangCite this: Biochemistry 1977, 16, 20, 4464–4469Publication Date (Print):October 1, 1977Publication History Published online1 May 2002Published inissue 1 October 1977https://pubs.acs.org/doi/10.1021/bi00639a021https://doi.org/10.1021/bi00639a021research-articleACS...
Cse4p is a structural component of the core centromere Saccharomyces cerevisiae and member conserved CENP-A family specialized histone H3 variants. The H4 allele hhf1-20 confers defects in chromatin structure mitotic chromosome transmission. We have proposed that interact through their respective fold domains to assemble nucleosome-like at centromeric DNA. To test this model, we targeted random mutations domain isolated three temperature-sensitive cse4 alleles an unbiased genetic screen. Two...
The genome of haploid Saccharomyces cerevisiae contains two nonallelic sets histone H3 and H4 genes. Strains with deletions each these loci were constructed by gene replacement techniques. Mutants containing either set viable, however meiotic segregants lacking both inviable. In cells no phenotypic expression the was observed; deletion mutants had wild-type growth rates, not temperature sensitive for growth, mated normally. However, diploids homozygous H3-H4 slightly defective in their cell...
The C-terminal portion of adenovirus E1A suppresses ras-induced metastasis and tumorigenicity in mammalian cells; however, little is known about the mechanisms by which this occurs. In simple eukaryote Saccharomyces cerevisiae, Ras2p, homolog h-ras, regulates mitogen-activated protein kinase (MAPK) cyclic AMP-dependent A (cAMP/PKA) signaling pathways to control differentiation from yeast form pseudohyphal form. When expressed yeast, region induced differentiation, was independent both MAPK...
The yeast CHA1 promoter is activated in the presence of serine or threonine. Activation requires Cha4p activator, and it results perturbation a nucleosome that incorporates TATA element under noninducing conditions. We show lacking amino terminus histone H3, constitutively active chromatin concomitantly perturbed. This derepression occurs absence elevated intracellular levels threonine not observed cells Rpd3p, Tup1p, H4. Furthermore, H3 primary activator this promoter, Cha4p, which we by...
The N-terminal domains of the histones H3 and H4 are highly conserved throughout evolution. Mutant alleles deleted for these were constructed in vitro examined function vivo Saccharomyces cerevisiae. Cells containing a single deletion allele either histone or viable. Deletion domain caused cells to become sterile temperature sensitive growth. normal cell cycle progression was also altered, as revealed by major delay through G2 + M periods. had only minor effects on mating...
An autonomously replicating segment, ARS, is located 293 base pairs downstream from the histone H4 gene at copy-I H3-H4 locus. The sequences needed for autonomous replication were defined by deletion analysis to include an ARS consensus sequence and additional 3'-flanking region. External deletions into yeast resulted in a loss of function. However, disruptions required domain either 10-base-pair linker-scanning substitutions or larger internal did not impair replication. Thus, dependent...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTranscription of bacteriophage λ DNA in vitro using purine nucleoside 5'-[γ-S]triphosphates as affinity probes for RNA chain initiationM. Mitchell Smith, Anthony E. Reeve, and Ru Chih C. HuangCite this: Biochemistry 1978, 17, 3, 493–500Publication Date (Print):February 7, 1978Publication History Published online1 May 2002Published inissue 7 February 1978https://doi.org/10.1021/bi00596a019RIGHTS & PERMISSIONSArticle Views27Altmetric-Citations30LEARN...
Esa1 is the only essential histone acetyltransferase (HAT) in budding yeast. It catalytic subunit of at least two multiprotein complexes, NuA4 and Piccolo (picNuA4), its function believed to be HAT activity. To examine role DNA damage repair, we isolated viable esa1 mutants with a range hypersensitivities toposide camptothecin. Here show that sensitivity these variety stresses inversely proportional their level H4 acetylation, demonstrating importance activity for resistance genotoxic...
By tethering their circular genomes (episomes) to host chromatin, DNA tumor viruses ensure retention and segregation of genetic material during cell divisions. Despite functional crystallographic studies, there is little information addressing the 3D structure these tethers in cells, issues critical for understanding persistent infection by viruses. Here, we have applied direct stochastic optical reconstruction microscopy (dSTORM) establish nanoarchitecture within cells latently infected...