A5063534862- Syphilis Diagnosis and Treatment
- Muscle Physiology and Disorders
- Genomics and Chromatin Dynamics
- Reproductive tract infections research
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Vector-borne infectious diseases
- RNA modifications and cancer
- Fungal and yeast genetics research
- Epigenetics and DNA Methylation
- Viral Infections and Vectors
- Monoclonal and Polyclonal Antibodies Research
- Vector-Borne Animal Diseases
- Biotin and Related Studies
- Reproductive System and Pregnancy
- Cardiomyopathy and Myosin Studies
- Female Genital Mutilation/Cutting Issues
- Plant Gene Expression Analysis
- Peptidase Inhibition and Analysis
- Neurogenetic and Muscular Disorders Research
- Autoimmune and Inflammatory Disorders
- Sex work and related issues
- RNA and protein synthesis mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Heat shock proteins research
The University of Texas Health Science Center at Houston
2009-2025
Freeman Hospital
2008
The University of Texas MD Anderson Cancer Center
1992-2005
University of Utah
1998-2000
Boston College
1999
University of Rochester
1996
University of British Columbia
1996
The University of Texas at Austin
1986
We report the cloning of a transcription-associated histone acetyltransferase type A(HAT A). This Tetrahymena enzyme is strikingly homologous to yeast protein Gcn5, putative transcriptional adaptor, and we demonstrate that recombinant Gcn5p possesses HAT activity. Both ciliate contain potential active site residues found in other acetyltransferases highly conserved bromodomain. The presence this domain nuclear A-type HATs, but not cytoplasmic B-type suggests mechanism whereby A directed...
MyoD1 is a nuclear phosphoprotein that expressed in skeletal muscle vivo and certain cell lines vitro; it has been shown to convert fibroblasts myoblasts through mechanism requiring domain with homology the myc family of proteins. The BC3H1 line expresses muscle-specific genes upon exposure mitogen-deficient medium, but does not express at detectable levels. To determine whether cells may regulatory functionally related MyoD1, cDNA library prepared from differentiated myocytes, was screened...
ABSTRACT Members of the MEF2 family transcription factors bind a conserved A/T-rich sequence in control regions many skeletal and cardiac muscle genes. To begin to assess roles different Mef2 genes gene expression vivo, we analyzed by situ hybridization patterns Mef2a, Mef2c Mef2d during mouse embryogenesis. We first detected MEF2C at day 7.5 postcoitum (p.c.) cells mesoderm that give rise primitive heart tube, making one earliest markers for lineage yet described. By 8.5, MEF2A, MEF2D mRNAs...
Repression of yeast a cell-specific genes by the global repressor Ssn6/Tup1 has been linked to specific organization chromatin. We report here that Tup1 directly interacts with amino-terminal tails histones H3 and H4, providing molecular basis for this connection. This interaction appears be required function because mutations in H4 weaken interactions cause derepression both DNA damage-inducible genes. Moreover, histone-binding domain coincides previously defined repression domain....
Investigation of
Transcriptional cascades that specify cell fate have been well described in invertebrates. In mammalian development, however, gene hierarchies involved determination of lineage are not understood. With the recent cloning MyoD family myogenic regulatory factors, a model system has become available with which to study dynamics muscle development. Myogenin, along other members family, possesses apparent ability redirect nonmuscle cells into lineage. This appears be due direct activation an...
Myogenin belongs to a family of regulatory factors that can activate myogenesis when transfected into nonmyogenic cells. A conserved DNA sequence, known as an E box, serves the target for binding and trans-activation by myogenin. Using 10T1/2 fibroblasts constitutively express myogenin cDNA, we show accumulates in nucleus but is unable initiate cells are maintained with transforming growth factor beta (TGF-beta) or high serum. Although final effect TGF-beta serum--inhibition myogenesis--was...
Ssn6–Tup1 regulates multiple genes in yeast, providing a paradigm for corepressor functions. Tup1 interacts directly with histones H3 and H4, mutation of these synergistically compromises Ssn6–Tup1-mediated repression. In vitro, preferentially underacetylated isoforms suggesting that histone acetylation may modulate functions vivo. Here we report hyperacetylation caused by combined mutations encoding the deacetylases (HDACs) Rpd3, Hos1, Hos2 abolishes Unlike HDAC do not affect repression,...
The yeast transcriptional adapter Gcn5p serves as a histone acetyltransferase, directly linking chromatin modification to regulation.Two human homologs of have been reported previously, hsGCN5 and hsP/CAF (p300/CREB binding protein [CBP]-associated factor).While was predicted be close the size contained an additional 356 amino-terminal residues unknown function.Surprisingly, we found that in mouse, both GCN5 P/CAF genes encode proteins containing this extended domain.Moreover, while shorter...
AbstractThe growth suppressor promyelocytic leukemia protein (PML) is disrupted by the chromosomal translocation t(15;17) in acute (APL). PML plays a key role multiple pathways of apoptosis and regulates cell cycle progression. The present study demonstrates that represses transcription functionally physically interacting with histone deacetylase (HDAC). Transcriptional repression mediated can be inhibited trichostatin A, specific inhibitor HDAC. coimmunoprecipitates significant level HDAC...
Abstract For over a century, investigation of Treponema pallidum subsp. , the spiral‐shaped bacterium that causes syphilis, was hindered by an inability to culture organism in vitro. A recent breakthrough has enabled continuous vitro growth this co‐culture with mammalian tissue cells. This article contains protocols needed T. standard laboratory environment. In addition, for growing and maintaining required cells, generating isogenic strains limiting dilution, quantitating darkfield...
The Tup1-Ssn6 corepressor complex in Saccharomyces cerevisiae represses the transcription of a diverse set genes. Chromatin is an important component Tup1-Ssn6-mediated repression. Tup1 binds to underacetylated histone tails and requires multiple deacetylases (HDACs) for its repressive functions. Here, we describe physical interactions with class I HDACs Rpd3, Hos2, Hos1. In contrast, no vivo interaction was observed between Tup-Ssn6 Hda1, II HDAC. We demonstrate that Rpd3 interacts both...
Post-translational modification of histones is a central aspect gene regulation. Emerging data indicate that at one site can influence second site. As example, histone H3 phosphorylation serine 10 (Ser<sup>10</sup>) facilitates acetylation lysine 14 (Lys<sup>14</sup>) by Gcn5 <i>in vitro</i> (1, 2). <i>In vivo</i>, precedes certain promoters. Whether globally affects acetylation, or whether it all sites in equally, not known. We have taken genetic approach to this question mutating...
summary Although passive infusion of plasma‐rich components containing white blood cell (WBC) antibodies are responsible for majority the reported transfusion‐related acute lung injury (TRALI) cases, minimum volume residual plasma, which might trigger TRALI, is not known. We report three cases TRALI where implicated donor component contained between 10 and 20 mL plasma. Two were related to transfusion red cells prepared in optimal additive solution, other was pooled buffy coat platelets. In...
Antigenic variation plays a vital role in the pathogenesis of many infectious bacteria and protozoa including Borrelia burgdorferi, causative agent Lyme disease. VlsE, 35 kDa surface-exposed lipoprotein, undergoes antigenic during B. burgdorferi infection mammalian hosts, is believed to be critical mechanism by which spirochetes evade immune clearance. Random, segmental recombination between expressed vlsE gene adjacent vls silent cassettes generates large number different VlsE variants...
Transcriptional cascades that specify cell fate have been well described in invertebrates. In mammalian development, however, gene hierarchies involved determination of lineage are not understood. With the recent cloning MyoD family myogenic regulatory factors, a model system has become available with which to study dynamics muscle development. Myogenin, along other members family, possesses apparent ability redirect nonmuscle cells into lineage. This appears be due direct activation an...
Myogenin is a muscle-specific transcription factor that can activate myogenesis; it belongs to family of factors share homology within basic region and an adjacent helix-loop-helix (HLH) motif. Although myogenin alone binds DNA inefficiently, in the presence widely expressed HLH proteins E12 E47 (encoded by E2A gene), forms heterooligomers bind with high affinity sequence known as kappa E-2 site. In contrast, lesser extent are both able site relatively efficiently homooligomers. To define...
Treponema pallidum subsp. is the causative agent of syphilis, a sexually transmitted disease characterized by widespread tissue dissemination and chronic infection. In this study, we analyzed proteome T. isoelectric focusing (IEF) nonequilibrating pH gel electrophoresis (NEPHGE) forms two-dimensional (2DGE), coupled with matrix-assisted laser desorption ionization-time flight (MALDI-TOF) analysis. We determined identity 148 protein spots, representing 88 polypeptides; 63 these polypeptides...