A5010930479- Cardiac Ischemia and Reperfusion
- Heart Rate Variability and Autonomic Control
- Cardiac Arrest and Resuscitation
- Mitochondrial Function and Pathology
- Respiratory Support and Mechanisms
- Neonatal Respiratory Health Research
- Cardiovascular and exercise physiology
- Anesthesia and Neurotoxicity Research
- Cardiac electrophysiology and arrhythmias
- Adipose Tissue and Metabolism
- Nitric Oxide and Endothelin Effects
- Non-Invasive Vital Sign Monitoring
- Muscle Physiology and Disorders
- Neuroscience of respiration and sleep
- Ion channel regulation and function
- Cardiovascular Function and Risk Factors
- Exercise and Physiological Responses
- Spaceflight effects on biology
- Cardiomyopathy and Myosin Studies
- Ion Channels and Receptors
- Mechanical Circulatory Support Devices
- Cardiovascular Health and Disease Prevention
- Neurobiology and Insect Physiology Research
- Infant Development and Preterm Care
- Physical Activity and Health
Mount Sinai Medical Center
2015-2024
St James's University Hospital
2020
Biophysical Society
2020
University of California, Davis
2020
University of Leeds
2020
Mount Sinai Hospital
2006-2018
University of Chile
2016
University of Miami
1987-2010
Miami Heart Research Institute
2001-2007
Intensive Care Society
1997
Abstract Mutations in the type 1 ryanodine receptor (RyR1), a Ca 2+ release channel skeletal muscle, hyperactivate to cause malignant hyperthermia (MH) and are implicated severe heat stroke. Dantrolene, only approved drug for MH, has disadvantages of having very poor water solubility long plasma half-life. We show here that an oxolinic acid-derivative RyR1-selective inhibitor, 6,7-(methylenedioxy)-1-octyl-4-quinolone-3-carboxylic acid (Compound 1, Cpd1), effectively prevents treats MH stroke...
Respiratory inductive plethysmography (RIP) is a well-accepted noninvasive technology for monitoring breathing patterns in adults. Prior attempts to calibrate this device babies have been fraught with technical difficulties, thereby limiting applications population. Recently, new method, qualitative diagnostic calibration (QDC), has shown provide accurate of tidal volume The QDC method based upon principles the isovolume maneuver and carried out during natural without specialized respiratory...
Neuronal intracellular Ca2+ homeostasis is critical to the normal physiological functions of neurons and neuronal dyshomeostasis has been associated with age related decline cognitive functions. Accumulated evidence indicates that underlying mechanism for this abnormal levels stimulate dysregulation signaling, which subsequently induces cell death. We examined in cortical (in vivo) hippocampal vitro) from young (3-mos), middle-age (12-mos), aged (24-mos) wild type C57BL6J mice. found a...
Background: Postoperative cognitive dysfunction (POCD) is a common disorder after general anesthesia in elderly patients, the precise mechanisms of which remain unclear. Methods: We investigated effect isoflurane with or without dantrolene pretreatment on intracellular calcium concentration ([Ca2+]i), reactive oxygen species (ROS) production, cellular lactate dehydrogenase (LDH) leak, calpain activity, and function using Morris water maze test young (3 months), middle-aged (12–13 aged (24–25...
Objective To determine whether a motion platform that imparts noninvasive periodic acceleration (pGz) forces to the body causes systemic vasodilation and changes local organ blood flow. Design Prospective paired blocked design. Setting Medical center research laboratory. Subjects Juvenile Yorkshire pigs. Interventions pigs (12 kg) were anesthetized, paralyzed, placed on oscillated at frequency of 4 Hz force ∼0.4 G. Measurements Main Results Regional flows, as assessed by colored...
Abstract The current study was undertaken to ascertain whether shift from supine prone posture alters the pattern of natural breathing in healthy fullterm newborns. Breathing patterns were measured and 20 infants using calibrated, noninvasive respiratory inductive plethysmography (RIP). values for components postures expressed as means (± SD) were, respectively, tidal volume (V T ), 14.1(± 3.2) 18.9(± 4.9)mL; mean inspiratory flow 26.7(± 11.5) 32.8(± 13.0)mL/s; minute ventilation 232(± 75)...
Diabetic cardiomyopathy (DCM) is a primary disease in diabetic patients characterized by diastolic dysfunction leading to heart failure and death. Unfortunately, even tight glycemic control has not been effective its prevention. We have found aberrant Ca2+ concentrations ([Ca2+]d), decreased glucose transport, elevated production of reactive oxygen species (ROS), increased calpain activity cardiomyocytes from murine model (db/db) type 2 diabetes (T2D). Cardiomyocytes these mice demonstrate...
Cellular and isolated vessel experiments have shown that pulsatile laminar shear stress to the endothelium produces significant release of mediators into circulation. Periodic acceleration (pG(z)) applied whole body in direction spinal axis adds pulses circulation, thereby increasing should also cause The purpose this study was determine whether addition circulation through pG(z) would be sufficient increase animals acutely how such a physical maneuver might affect coagulation factors....
Low-amplitude pulses to the vasculature increase pulsatile shear stress endothelium. This activates endothelial nitric oxide (NO) synthase (eNOS) promote NO release and endothelial-dependent vasodilatation. Descent of dicrotic notch on arterial pulse waveform a-to-b ratio (a/b; where a is height amplitude b above end-diastolic level) reflects vasodilator (increased a/b) vasoconstrictor effects (decreased due level change. Periodic acceleration (pG(z)) (motion supine body head foot platform)...
Background . Heart rate variability (HRV) reflects neural balance between sympathetic and parasympathetic autonomic nervous systems (ANS). Reduced HRV occurs in several chronic diseases physical inactivity. External addition of pulses to the circulation restores HRV. A new method add can be accomplished with a passive simulated jogging device (JD). We hypothesized that application JD might increase seated supine postures heterogeneous group volunteer subjects. Methods Twenty ambulatory...
This study characterizes the pharmacokinetics of bumetanide after an intravenous dose 0.05 or 0.10 mg/kgto 14neonates (weight range 820-4,000 g; gestational age 26-40 weeks) during first week life. Blood samples and urine were collected for up to 12 h dosing. Estimated serum clearance was 0.2–1.0 ml/min·kg (range), volume distribution 0.22 1/kg (range 0.11–0.32 1/kg), harmonic mean half-life 6–7 4–19 h). Nonrenal accounted 58–97% with presence certain oxidative metabolites in urine. These...
Flexible fiberoptic bronchoscopy with bronchoalveolar lavage was performed in 16 pediatric patients the acquired immunodeficiency syndrome (AIDS) and deterioration pulmonary function suggestive of opportunistic infection. In 62% Pneumocystis carinii identified. Culture results showed a pure growth Pseudomonas aeruginosa for one patient addition to carinii. Bronchoscopy well tolerated, few complications even among significant tachypnea hypoxia. Because its relative safety effectiveness, this...
Abstract Measurement of breath amplitude (BA) and similarly tidal volume (V T ) in newborn infants is the standard for detection apnea hypopneas. The purpose our study was to compare accuracy BA by three frequently utilized noninvasive respiratory monitors: inductive plethysmography (RIP), mercury silastic strain gauges (SG), impedance pneumography (IP). Twenty healthy full‐term were studied both supine prone postures. RC AB gain factors RIP obtained using qualitative diagnostic calibration...
Duchenne muscular dystrophy (DMD) is a genetic disorder caused by the absence of dystrophin in both skeletal and cardiac muscles. This leads to severe muscle degeneration, dilated cardiomyopathy that produces patient death, which most cases occurs before end second decade. Several lines evidence have shown modulators nitric oxide (NO) pathway can improve function mdx mouse, mouse model for DMD. Whole body periodic acceleration (pGz) produced applying sinusoidal motion supine humans standing...
The recognition that oxidative stress is a major component of several chronic diseases has engendered numerous trials antioxidant therapies with minimal or no direct benefits. Nanomolar quantities nitric oxide released into the circulation by pharmacologic stimulation eNOS have properties but physiologic as through increased pulsatile shear endothelium not been assessed. present study utilized non-invasive technology, periodic acceleration (pGz) increases such upregulation cardiac occurs, We...