A5032825330- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Mitochondrial Function and Pathology
- Protein Structure and Dynamics
- RNA Research and Splicing
- RNA modifications and cancer
- Force Microscopy Techniques and Applications
- HIV Research and Treatment
- Advanced Electron Microscopy Techniques and Applications
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ubiquitin and proteasome pathways
- Near-Field Optical Microscopy
- Advanced biosensing and bioanalysis techniques
- Fungal and yeast genetics research
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
Northeastern University
2013-2017
The regulation of chromatin structure in eukaryotic cells involves abundant architectural factors such as high mobility group B (HMGB) proteins.It is not understood how these control the interplay between genome accessibility and compaction.In vivo, HMO1 binds promoter coding regions most ribosomal RNA genes, facilitating transcription possibly stabilizing absence histones.To understand performs functions, we combine single molecule stretching atomic force microscopy (AFM).By HMO1-bound DNA,...
We obtain accurate three-dimensional persistence length measurements for DNA and DNA–protein complexes using liquid AFM imaging, validated by optical tweezers.
Human mtDNA contains three promoters, suggesting a need for differential expression of the mitochondrial genome. Studies transcription have used reductionist approach, perhaps masking regulation. Here we evaluate from light-strand (LSP) and heavy-strand (HSP1) promoters using templates that mimic their natural context. These studies reveal sequences upstream, hypervariable in human population (HVR3), downstream HSP1 start site required maximal yield. The carboxy-terminal tail TFAM is...
Mitochondrial transcription factor A (TFAM) is an abundant human mitochondrial High Mobility Group Box (HMGB) protein. Similar to several nuclear HMGB proteins, TFAM has two domains that...
Abstract HMO1 proteins are abundant Saccharomyces cerevisiae (yeast) High Mobility Group Box (HMGB) protein (Kamau, Bauerla & Grove, 2004). HMGB nuclear which known to be architectural (Travers, 2003). possesses two box domains. It has been reported that double induce strong bends upon binding DNA. is also believed they play an essential role in reorganizing chromatin and, therefore, likely involved gene activation. To characterize DNA we combine single molecule stretching experiments and...
Human mtDNA contains three promoters, suggesting a need for differential expression of the mitochondrial genome. Studies transcription have used reductionist approach, perhaps masking regulation. Here we evaluate from light-strand (LSP) and heavy-strand (HSP1) promoters using templates that mimic their natural context. These studies reveal sequences upstream, hypervariable in human population (HVR3), downstream HSP1 start site required maximal yield. The carboxy-terminal tail TFAM is...