The feasibility of an in situ tissue-engineering method employing cell-based therapy with autologous periodontal ligament-derived cells was investigated. Periodontal ligament were obtained from six beagle dogs. fenestration defects (6 × 4 mm) created bilaterally at a location 6 mm apical to the marginal alveolar crest maxillary canines. Alkaline phosphatase-positive (3 105 cells) seeded onto collagen sponge scaffold just before implantation. One defect filled cell-scaffold construct, and...

Exposure to foreign particles sometimes causes inflammatory reactions through production of cytokines and chemoattractants by phagocytic cells. In this study, we focused on macrophage migration inhibitory factor (MIF) evaluate its pathophysiological role in the process. Immunohistochemical analysis human pseudosynovial tissues retrieved at revision total hip arthroplasty showed that infiltrating mononuclear multinuclear cells were positively stained both an anti‐CD68 antibody anti‐human MIF...

A novel, injectable bone tissue engineering material was developed that consisted of β-tricalcium phosphate (β-TCP) beads as the solid phase and alginate gel phase. To prepare instantaneously formed composite scaffold, an aqueous calcium chloride solution dried on surface β-TCP crosslinked with alginic acid sodium solution, thereby forming stable which were via a syringe. This biodegradable three-dimensional (3D) could be used scaffold for engineering. In particular, 2.0 wt% concentration...

Synthetic biomaterials have been developed and used for bone grafting. Here, we a biodegradable sponge composite tissue engineering by combining β-tricalcium phosphate (β-TCP) collagen. In addition, sought to determine the optimal β-TCP granules/ collagen ratio evaluating formation in vivo. Porous granules were mixed with atelocollagen hydrochloride solution at various ratios — 0.02, 0.05, 0.1, 0.2 g/ mL. The resultant mixtures freeze-dried subjected dehydrothermal treatment vacuo. final...

The increase in oxidative stress that accompanies aging has been implicated the abnormal advance of and onset various systemic diseases. However, details what effects on saliva secretion are not known. In this study, naturally mice were used to examine stimulated whole flow rate, serum stress, antioxidant level, submandibular gland H-E staining, immunofluorescence staining investigate effect volume relationship with as well structure salivary tissue. rate decreased significantly age. Also,...

Oral dryness, a serious problem for the aging Japanese society, is induced by aging-related hyposalivation and causes dysphagia, dysgeusia, inadaptation of dentures, growth oral Candida albicans. Oxidative stress clearly plays role in decreasing saliva secretion treatment with antioxidants such astaxanthin supplements may be beneficial. Therefore, we evaluated effects on secretory function mice. The flow increased astaxanthin-treated mice 72 weeks after administration while that control...

The search for hepatitis C virus (HCV) in body fluids other than blood is important when assessing possible nonparenteral routes of viral transmission. However, the role oral HCV transmission remains controversial. Here we quantitatively determined RNA saliva and gingival crevicular fluid (GCF) anti-HCV-positive patients. Most patients (14 18; 78%) whose specimens were negative had their GCF. (20 26; 77%) higher levels GCF saliva. Although there was not a statistically significant...

The objective of this study was to investigate the feasibility biodegradable gelatin–β-tricalcium phosphate (β-TCP) composites as a cell scaffold and controlled-release carrier basic fibroblast growth factor (bFGF) suitable for inducing bone regeneration at segmental defect. composite gelatin sponge β-TCP granules had an interconnected pore structure with average size 340 µm. provided controlled release bFGF over 2 weeks. Segmental, critical-sized, defects 20 mm length were created in ulnas...

Oral lichen planus is a chronic inflammatory disease that affects the mucous membrane of oral cavity and can contribute to development other diseases. Inflammation in T-cell-mediated autoimmune acts through cytotoxic CD8(+) T cells trigger apoptosis keratinocytes. However, specific cause remains unknown no effective medical treatment has yet been established. Astaxanthin carotenoid pigment with capacity for anti-inflammatory anti-oxidant activities. In this study, we evaluated whether...

Bone resorption and formation are dynamic processes that occur in both normal injured bone tissues. Regulation of these is mediated at the local level by cytokines growth factors. Macrophage migration inhibitory factor (MIF) one proinflammatory activates macrophages regulates production other cytokines, such as tumour necrosis factor‐α interleukin‐1. We here demonstrate, reverse transcription–polymerase chain reaction, high expression MIF mRNA murine osteoblasts obtained from mouse neonatal...

A moderately halophilic, obligate alkaliphile (growth range pH 8–12), designated strain YN-1T, was isolated from indigo balls obtained Ibaraki, Japan. The cells of the isolate stained Gram-positive, and were aerobic, non-motile, sporulating rods which grew optimally at 9. in 3–14 % NaCl with optimum growth 5 NaCl. It hydrolysed casein Tweens 20, 40 60, but not gelatin, starch, DNA or pullulan. Its major isoprenoid quinone MK-7 its cellular fatty acid profile mainly consisted anteiso-C15 : 0,...

Fibronectin (Fn) and type I collagen (Col) were immobilized on a surface of hydroxyapatite (HAP) ceramic by coprecipitation with calcium phosphate in supersaturated solution prepared mixing clinically approved infusion fluids. These proteins the precipitate formed composite layer. As result, firmly as not to be released completely for 3 d physiological salt solution. When human mesenchymal stem cells (hMSCs) cultured HAP differentiation medium supplemented dexamethasone,...

For many years, bone graft substitutes have been used to reconstruct defects in orthopedic and dental fields. However, synthetic such as hydroxyapatite or β-tricalcium phosphate no osteoinductive osteogenic abilities. Bone tissue engineering has also promoted an alternative approach regenerating tissue. To succeed engineering, osteoconductive scaffolding biomaterials should provide a suitable environment for cells local controlled release of growth factors. In addition, the scaffold...

Surface modification of titanium has been extensively investigated in implant science and technology an effort to improve its osteoconductivity. The rate protein adsorption on surfaces is known vary depending the chemistry, structure, morphology, titanium-specific biological aging surface. It thus desirable modify smooth miniimplants used as orthodontic anchors immediately prior use. In this study, we have developed a simple surface alloy that improves biofunctional activity. Ti-6Al-4V disk...

The objective of this study was to investigate the odontoblastic differentiation dental pulp stem cells (DPSC) by biodegradable hydrogels incorporating simvastatin micelles, both in vitro and vivo. Simvastatin (ST) incorporated into micelles gelatin grafted with L-lactic acid oligomers (LAo) allow water-solubilization. simvastatin-LAo-grafted (LAo-g-gelatin) were mixed gelatin, followed chemical crosslinking form (ST Mi/GH). ST Mi released from hydrogel granules (GH) through enzymatic...

The distribution of ferritin has been studied in many tissues, but not yet established on the cellular level. We investigated liver, spleen and bone marrow using immunoperoxidase method, compared it with that hemosiderin. also examined changes these proteins after phlebotomy iron overload. In normal rats, was seen centrilobular hepatocytes, Kupffer cells, macrophages red white pulp central marrow. Hemosiderin observed almost exclusively partly tangible body pulp. After phlebotomy, neither...

Two Gram-variable, aerobic, motile, rod-shaped, endospore-forming bacterial strains, M4-2T and M4-1, were isolated from soil samples collected Oblast Magadan, Russian Far East, as micro-organisms antagonistic to the psychrophilic phytopathogenic fungus Typhula ishikariensis. Strains M4-1 identified members of genus Paenibacillus by phenotypic phylogenetic analyses based on 16S rRNA gene sequences. The strains contained anteiso-C15 : 0 major fatty acid (63.0–64.7 %) MK-7 isoprenoid quinone....

Gatifloxacine (GFLX)-containing poly(lactide-co-glycolide) (PLGA) was introduced to the pores and surfaces of porous β-tricalcium phosphate (βTCP) granules by melt compounding whereby no toxic solvent used. The granular composite GFLX-loaded PLGA βTCP released GFLX for 42 days in Hanks' balanced solution exhibited sufficient vitro bactericidal activity against Streptococcus milleri Bacteroides fragilis at least 21 days. For vivo evaluation, implanted dead space created debridement...