Abstract Islets transplantation, as a treatment of type 1 diabetes, faces challenges, including the loss islets in process isolation and pre-transplantation due to cellular stresses-induced apoptosis. Accordingly, optimization culture plays decisive role transplantation success. In this study, we evaluated effect nobiletin on cultured human islets. Isolated were treated by different concentrations for 24 72 hours. Then, viability, apoptosis, insulin C-peptide secretion, apoptosis markers...

The successful ex vivo expansion of T-cells in great numbers is the cornerstone adoptive cell therapy. We aimed to achieve most optimal T-cell condition by comparing at various seeding densities, IL-2 concentrations, and bead-to-cell ratios. we first expanded peripheral blood mononuclear cells (PBMCs) a healthy donor range 20 500 IU/mL 125 × 103 1.5 106 cell/mL, 1:10 10:1 B:C (Bead-to-cell) ratios compared results. then PBMC three donors using optimized conditions examined growth kinetics....

Background: Metastasis is a major cause of death from cancer in triple-negative breast (TNBC). Apoptosis evasion critical feature metastatic tumor cells. Chemopreventive and apoptotic potential curcumin has been shown cancer. However, the precise mechanism these effects against cells not clearly addressed yet. Methods: 4T1 cell line was used for induction animal model Primary were extracted subcutaneous lung cancerous mice, respectively. MTT assay to determine effect on viability...

Abstract Background Relapse and metastasis in breast cancer are linked to stem cells (CSCs) resistant anticancer therapies. The presence of stem‐like (CSLCs) their ability self‐renew is determined by vitro spheroid formation. Aims Many studies have found that frankincense has impacts, although these effects on CSLCs never been evaluated. Methods results A population heterogeneous tumor was extracted from the mass after generating an animal model triple‐negative (TNBC). Spheroid formation...

Abstract Objective Osteopontin (OPN) is a well-known glycoprotein involved in numerous pathobiological processes, including cancer. Despite having five splice variants for osteopontin mice, the main focus of most studies has been on total OPN (tOPN). There are some other variants, but expression osteopontin-5 (OPN5) not addressed mouse cancer cells. Therefore, this study sought to evaluate OPN5 breast Results The primary and metastatic cells mice was confirmed our study. These findings...

Abstract Poor prognosis and low survival are commonly seen in patients with glioblastoma multiforme (GBM). Due to the specific nature of solid tumors such as GBM, delivery therapeutic agents tumor sites is difficult. So, one major challenges treatment these a selection appropriate method for drug delivery. Mesenchymal stem cells (MSCs) have unique characteristic migration toward tissue. In this regard, present study examined antitumor effects manipulating human placenta‐derived mesenchymal...

Protection of isolated pancreatic islets against hypoxic and oxidative damage-induced apoptosis is essential during a pretransplantation culture period. A beneficial approach to maintain viable functional the coculture period with mesenchymal stem cells (MSCs). Hypoxia preconditioning MSCs (Hpc-MSCs) for short time stimulates expression secretion antiapoptotic, antioxidant, prosurvival factors. The aim present study was evaluate survival function human cocultured Hpc-MSCs. Wharton’s...

Background: Hyperlipidemia and low antioxidant levels is one the diabetes side effects. Some studies have indicated possible effects of nutrients on improvement hyperlipidemia, by their antioxidants ingredients. Objectives: The aim present study was to evaluate effect synthetic antioxidant, tempol, blood lipid profiles glucose in healthy diabetic rats. Materials Methods: Adult Wistar rats were randomly divided four experimental groups including, control, receiving tempol groups. Diabetes...

Abstract Background Mesenchymal stem cells (MSCs) have been suggested as an appropriate source for diabetes cell-based therapies. The high proliferation and differentiation capacity of fetal MSCs the role pancreatic-derived (FPMSCs) in islet generation make them good candidates treatment. To manufacture clinical-grade MSCs, animal-free culture protocols are preferred. current study aimed to establish a xeno-free/GMP-compliant protocol FPMSCs manufacturing. focus was on effects bovine serum...

Abstract Background Cytokine-induced killer (CIK) cells have shown promising results in adoptive immunotherapy. However, serum may play a determining role the large-scale expansion of these for clinical applications. According to Good Manufacturing Practice (GMP) guidelines reduce use animal products cell-based therapies; therefore, this study sought investigate impact origin and reduced concentration on pattern cell function. Methods Peripheral blood mononuclear (PBMCs) isolated from...

Background: Chromosomal breakpoints are the most common cause of hereditary diseases and cancers. Today, many standard clinical methods such as cytogenetic PCR based techniques used which have limitation regarding detection resolution. Chromosome conformation capture is a method for detecting gene proximity chromosomal rearrangements. Materials Methods: In this study, SKW3 cell line was t(8;14)(q24;q11) using 3Cbased technique. 3C library preparation. For Inverse PCR, two regions were...

Background: DNA probes have been widely used as diagnostic tools for translocations. This study sought to design a screening tool using ssDNA and chromosome conformation capture (3C) library fragment hybridization. Method: The authors focused on developing probe the juxtaposed region of MYC TRD. Fragments gene with thiol modification (MYC–Au NP probe) were functionalized by gold nanoparticles (Au NPs). Then TRD immobilized nitrocellulose surface. Hybridization between 3C fragments SKW3 cells...

Type 1 diabetes is a metabolic disorder caused by the loss or dysfunction of β-cells in pancreas. Organ shortage critical concern diabetic patients need beta islet transplantation. Tissue engineered islets are promising alternatives to traditional organ Recent progress stem cell biology and gene cloning techniques has raised hopes for generation insulin producing cells (IPCs) without immunosuppression. The purpose this study was produce IPCs using human adipose-derived (hADSCs)...

Background: Photodynamic Therapy (PDT) is considered as one of the alternative treatments for Head and Neck Squamous Cell Carcinoma (HNSCC). P53 mRNA a tumor suppressor gene whose mutation increases likelihood uncontrolled cancer-like divisions. The CCND1 oncogenic production cyclin D1 tumorigenic protein. Objectives: This study aimed to determine effect PDT using toluidine blue photosensitizer on levels in HNSCC cell line. Methods: A human line from NCBI.C196 designation HN5 was used. Cells...

Background: Preclinical development of new drugs for cancer immunotherapy requires preconditioning total body irradiation (TBI) mice to be humanized via hematopoietic stem cell transplantation. To assess the effect TBI, we detected reactive oxygen species (ROS), Annexin V, propidium iodide (PI) level in bone marrow samples by flow cytometer. Methods: We divided all NOG between irradiated (n = 20) and control groups 10) two time points. Irradiated were exposed 3.5 Gy radiation. After...

Abstract Background DNA probes have been widely used as diagnostic tools for chromosomal translocations in malignancies. PCR-based methods often fail to detect such MYC/TRD chronic lymphocytic leukemia. In addition, microscopic techniques cannot be helpful due size detection limitations. This study sought design a screening tool using immobilized ssDNA on nitrocellulose membrane followed by 3C library fragments hybridization. Results Hence, we focused developing suitable 27 bp specific probe...

Abstract Background Radiation is used for myeloablation in mice's bone marrow (BM) hematopoietic stem cells (HSC) engraftment experiments. To assess post-radiation damage to the BM, an accessible and convenient marker needed. This study comparatively evaluated flow cytometer's effectiveness detecting ROS Annexin V-PI level. Methods We divided 30 NOG mice between irradiated (n = 20) control groups 10) each time point. After sacrificing BM samples were collected, percentage of annexin V, PI,...

Stem cells have great potential to be applied as a treatment for various types of disorders. These exert therapeutic effects by modulating the immune system with capability secrete cytokines and chemokines. Previous studies indicated that stem could used agent different complaints, such gastrointestinal diseases. For long time now, researchers moved toward cells' clinical application in this context. With increasing number trials cell therapy disease, it is now evaluate these trials' status....

Abstract Background: DNA probes have been widely used as diagnostic tools for chromosomal translocations in malignancies. PCR-based methods often fail to detect such MYC/TRD chronic lymphocytic leukemia. In addition, microscopic techniques cannot be helpful due size detection limitations. This study sought design a screening tool using immobilized ssDNA on nitrocellulose membrane followed by 3C library fragments hybridization. Results: Hence, we focused developing suitable 27 bp specific...