A5101931190- Wound Healing and Treatments
- Plant and animal studies
- Insect and Arachnid Ecology and Behavior
- Skin and Cellular Biology Research
- Tissue Engineering and Regenerative Medicine
- Ecology and Vegetation Dynamics Studies
- Chemotherapy-related skin toxicity
- Cancer Cells and Metastasis
- Insect and Pesticide Research
- Dermatologic Treatments and Research
- Thermoregulation and physiological responses
- Colorectal and Anal Carcinomas
- Electrospun Nanofibers in Biomedical Applications
- Cellular Mechanics and Interactions
- Diverticular Disease and Complications
- Anorectal Disease Treatments and Outcomes
- Plant Parasitism and Resistance
- Burn Injury Management and Outcomes
- Corneal Surgery and Treatments
- RNA Interference and Gene Delivery
- Colorectal Cancer Treatments and Studies
- Abdominal Surgery and Complications
- Silk-based biomaterials and applications
- Mesenchymal stem cell research
- Surgical Sutures and Adhesives
Charles University
2012-2025
University Hospital Kralovske Vinohrady
2005-2017
Czech Academy of Sciences
2002-2007
University of South Bohemia in České Budějovice
2006
Czech Academy of Sciences, Institute of Molecular Genetics
1990-2002
Abstract With the rising threat to insect pollinators and upcoming pollinator crisis, it is important know how contribute pollen transfer. The contributions of individual taxa transfer depend both on their abundance much each can carry, with overall importance being a multiplication these two values. Here, we quantified load across diverse spectrum variation in over 11 years. We found that, while was relatively small among (compared high variability individuals within taxon), visitation...
Significance Biparental care is a rare strategy of offspring protection in insects. We discovered case biparental bees, Ceratina nigrolabiata . Benefits were identified for both males and females; yet, we found that an extraordinarily high proportion unrelated to guard males. This because females C. are polyandrous store sperm. Therefore, the most important benefit increased mating opportunities. suggest female polyandry prolonged receptivity supported origin and/or maintenance concomitant...
A number of implantable biomaterials derived from animal tissues are now used in modern surgery. Xe-Derma is a dry, sterile, acellular porcine dermis. It has remarkable healing effect on burns and other wounds. Our hypothesis was that the natural biological structure plays an important role keratinocyte proliferation formation epidermal architecture vitro as well vivo . The bioactivity studied by cell culture assay. We analyzed growth differentiation human keratinocytes cultured Xe-Derma, we...
Abstract Biparental care is very rare in insects, and it was well-documented only one bee species to this date – Ceratina nigrolabiata . However, biparental recently discovered species, detailed description of natural history missing. Here, we describe the nesting cycle C. Pairs are established before female starts offspring provisioning. After provisioning finished (when youngest reached larval stage), male abandons nest. Males which present nests where already brood cells, probably mainly...
Abstract Background Transformed phenotypes are common to cell lines derived from various cancers. Proteome profiling is a valuable tool that may reveal uncharacteristic in transformed cells. Changes expression of glutathione S-transferases (GSTs) and other proteins interacting with (GSH) model could be particular interest. Methods We compared the breast EM-G3, HCC1937, MCF7 MDA-MB-231 using 2-D electrophoresis (2-DE). further separated GSH-binding affinity chromatography GSH-Sepharose 4B,...
The aim of this study was to ascertain whether repeated local cooling induces the same or different adaptational responses as whole body cooling. Repeated legs (immersion into 12 degrees C water up knees for 30 min, 20 times during 4 weeks = cold adaptation - LCA) attenuated initial increase in heart rate and blood pressure currently observed control subjects immersed knees. After LCA skin temperature decrease tended be lower, indicating reduced vasoconstriction. Heart systolic appeared...
Abstract We performed a 2‐DE analysis of proteins the newly established spontaneously immortalized clonal cell line EM‐G3 derived from primary lesion infiltrating ductal breast carcinoma. cells may represent progenitors mammary epithelial in early phase cancerogenesis. compared protein profile with populations normal (NME), and determined phenotype both types cells. NME are mixture main epithelia, myoepithelial luminal The cancer has unique basal‐like phenotype. identified that differently...
Biological meshes are biomaterials consisting of extracellular matrix that used in surgery particularly for hernia treatment, thoracic wall reconstruction, or silicone implant-based breast reconstruction. We hypothesized combination matrices with autologous mesenchymal stem cells repair would result increased vascularization and strength incorporation. cultured adipose-derived harvested from the inguinal region Wistar rats on cross-linked noncross-linked porcine matrices. In 24 rats, a...
Abstract Background Breast carcinomas represent a heterogeneous group of tumors diverse in behavior, outcome, and response to therapy. Identification proteins resembling the tumor biology can improve diagnosis, prediction, treatment selection, targeting Since beginning post-genomic era, focus molecular gradually moved from genomes proteomes their functionality. Proteomics potentially capture dynamic changes protein expression integrating both genetic epigenetic influences. Methods We...
Local application of adipose-derived mesenchymal stem cells (ADSC) represents a novel approach for the management perianal fistula in patients with Crohn's disease. A randomised study on an animal model was performed to investigate efficacy and detect distribution implanted ADSCs by bioluminescence (BLI).A caecostomy used as 32 Lewis rats. The were isolated from transgenic donor expressing firefly luciferase. Animals randomly assigned groups given injections 4 × 106 (n = 16, group A) or...
Animal-pollinated plants face a high risk of pollen loss during its transfer. To limit the negative effect losses by consumption and heterospecific transfer, plant species may adjust stratify their availability day (i.e., "schedule" presentation) attract pollinators in specific time frames.We investigated diurnal patterns pollinator visitation three coflowering species: Succisa pratensis with open flowers accessible pollen, pollinated mainly pollen-feeding hoverflies; Centaurea jacea less...
The purpose of this study was to compare, by means in vitro cultivation technique, five marketed brands wound covers used the treatment burns and other skin defects (Biobrane(®), Suprathel(®), Veloderm(®), Xe-Derma(®), Xenoderm(®)) for their ability stimulate keratinocyte growth, stratification, differentiation. In three independent experiments, human keratinocytes were grown on tested organotypic cultures 3T3 feeder layer technique. Vertical paraffin sections with processed using...
Abstract Plants have evolved complex flowers that differ in visual traits such as colour, size and shape, to attract pollinators. However, pollinators seem respond differently individual traits. Our objective was unravel the effect of their interplay forming pollinator preferences. We measured preferences naive drone flies, Eristalis tenax (Syrphidae), for artificial differing flower symmetry, controlled conditions untangle effects on behaviour. showed highest selectivity followed by size,...
5-fluorouracil (5-FU) is one of the most commonly used antineoplastic drugs in anticancer therapy. The hand-foot (HF) syndrome (palmar-plantar erythrodysesthesia) an adverse effect frequently related to long-term i.v. administration 5-FU or its orally applicable prodrug capecitabine. Its severity can even lead interruption otherwise effective Tentative practice some clinics has shown that topical application 10% uridine ointment beneficial for calming down HF syndrome. This study focused on...
The limited amount of donor sites and loss dermis are major challenges in the therapy extensively burned patients. Here, we present a complex treatment approach an eight-year-old boy with full-thickness burns on 90% total body surface area, using simple efficient techniques tissue engineering. To obtain sufficient skin for grafting repeatedly harvested same anatomical areas. Acceleration site healing was achieved by suspension noncultured autologous cells (NASC) acellular porcine (Xe-Derma...