A5066444921- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Identification and Quantification in Food
- Microbial bioremediation and biosurfactants
- Toxic Organic Pollutants Impact
- Bacteriophages and microbial interactions
- Cancer therapeutics and mechanisms
- Antibiotic Resistance in Bacteria
- Microbial Natural Products and Biosynthesis
- Atmospheric and Environmental Gas Dynamics
- Global Energy and Sustainability Research
- Bioactive Compounds and Antitumor Agents
- Trypanosoma species research and implications
- Plant Micronutrient Interactions and Effects
- Enzyme Production and Characterization
- Bacterial Genetics and Biotechnology
- Sugarcane Cultivation and Processing
- Toxin Mechanisms and Immunotoxins
- Vector-Borne Animal Diseases
- Aquaculture disease management and microbiota
- Bacillus and Francisella bacterial research
- Chemical Analysis and Environmental Impact
- Cancer Research and Treatments
- Nitrogen and Sulfur Effects on Brassica
- Plant tissue culture and regeneration
Jamia Millia Islamia
2019-2020
Department of Biotechnology
2019-2020
University of Delhi
2009-2017
Institute of Medical Sciences
2016
Jubilant Life Sciences (India)
2013-2014
This paper presents the characterization of microbial community responsible for in-situ bioremediation hexachlorocyclohexane (HCH). Microbial structure and function was analyzed using 16S rRNA amplicon shotgun metagenomic sequencing methods three sets soil samples. The samples were collected from a HCH-dumpsite (450 mg HCH/g soil) comprised HCH/soil ratio 0.45, 0.0007, 0.00003, respectively. Certain bacterial; (Chromohalobacter, Marinimicrobium, Idiomarina, Salinosphaera, Halomonas,...
Aims: To locate a high-dose point hexachlorocyclohexane (HCH)-contaminated site, to identify HCH-degrading bacteria in it and assay HCH-decontamination by biostimulation. Methods Results: Bacteria were isolated serial dilution method from HCH-contaminated soil samples collected areas near an HCH-manufacturing unit its dumpsite North India. After confirming the presence of indigenous HCH-degraders (seven 24 strains), ex situ biostimulation experiment was conducted. For this, residue levels...
A Gram-stain-negative, motile, red pigmented, rod-shaped bacterium, designated strain LP43(T), was isolated from hexachlorocyclohexane (HCH)-contaminated soil sediment (Lucknow, India). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate formed a cluster with genus Pontibacter in phylum Bacteroidetes sequence similarities ranging 92.9 to 97.0 % species of Pontibacter. The DNA G+C content LP43(T) 59.1 mol%. polar lipid profile showed presence...
An orange-pigmented bacterial strain, designated LP100(T), was isolated from hexachlorocyclohexane-contaminated soil (Lucknow, India). A neighbour-joining tree based on 16S rRNA gene sequences showed that strain LP100(T) occupied a distinct phylogenetic position in the Pontibacter species cluster, showing highest similarity with lucknowensis DM9(T) (97.4 %). Levels of to strains other ranged between 94.0 and 96.8 %. Strain contained MK-7 as predominant menaquinone sym-homospermidine major...
A halotolerant, Gram-negative, rod-shaped and light-red-pigmented bacterium, designated LP51 T , was isolated from pond sediment near a hexachlorocyclohexane dumpsite located at Chinhat, Lucknow, India. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain formed distinct phyletic clade along with the members of genus Pontibacter. The sequence similarity to Pontibacter ranged 94.2 99.4 %. cells were motile, aerobic catalase- oxidase-positive. major fatty acids iso-C 15 :...
Sphingobium indicum B90A, an efficient degrader of hexachlorocyclohexane (HCH) isomers, was isolated in 1990 from sugarcane rhizosphere soil Cuttack, India. Here we report the draft genome sequence this bacterium, which has now become a model system for understanding genetics, biochemistry, and physiology HCH degradation.
Strain RK1(T), a Gram-stain-negative, non-spore-forming, rod-shaped, non-motile bacterium was isolated from hexachlorocyclohexane (HCH) dumpsite, Lucknow, India. 16S rRNA gene sequence analysis revealed that strain RK1(T) belongs to the family Sphingobacteriaceae and showed highest similarity Parapedobacter koreensis Jip14(T) (95.63%). The major cellular fatty acids of were iso-C15:0, summed feature 3 (C16:1ω7c and/or C16:1ω6c), iso-C17:0 3-OH, 9 (10-methyl C16:0 iso-C17:1ω9c), iso-C15:0...
A Gram-negative-staining, aerobic, non-motile, non-spore-forming, rod-shaped and yellow-pigmented bacterium, designated R11HT, was isolated from a soil sample collected hexachlorocyclohexane dumpsite located at Ummari village, Lucknow, Uttar Pradesh, India. The 16S rRNA gene sequence similarity between strain R11HT the type strains of species genus Sphingopyxis with validly published names ranged 93.75 to 97.85 %. Strain showed highest indica DS15T (97.85 %), followed by soli JCM15910T...
A Gram-stain-negative, pinkish-red, rod-shaped bacterium designated strain NKM1T was isolated from soil samples contaminated with hexachlorocyclohexane isomers, collected Ummari village, Lucknow, Uttar Pradesh, India. The characterized by a polyphasic taxonomic approach. 16S rRNA gene sequence analysis showed that clustered exclusively members of the genus Pontibacter family Cytophagaceae, phylum Bacteroidetes. similarity to type strains ranged 96.7 93.8 %, highest found odishensis JC130T...
Here, we report the draft genome sequence (4.2 Mb) of Sphingobium quisquiliarum strain P25(T), a natural lin (genes involved in degradation hexachlorocyclohexane [HCH] isomers) variant genotype, isolated from heavily contaminated (450 mg HCH/g soil) HCH dumpsite.
A novel bacterial strain, designated LP91T, was isolated from an agricultural field contaminated with hexachlorocyclohexane (HCH) isomers at Ummari Village, Lucknow, Uttar Pradesh, India. Cells of the strain were aerobic, short rod or coccoid, Gram-stain-negative and non-motile. Colonies initially transparent but time changed to a creamy white colour. Phylogenetic analysis based on 16S rRNA marker gene showed that it closely associated Paracoccus aestuariivivens GHD-30T (99.1 %) limosus...
An improved protocol was established for hairy root induction in Rauwolfia serpentina using two strains of Agrobacterium rhizogenes MTCC 532 and 2364. Hairy efficiency standardized based on infection time, co-cultivation period, OD600 acetosyringone concentration. Higher transformation (31%) 2364 (24%) with 30 min 72 h period 0.6 OD600. Transformation further enhanced to 55 % 125 μM acetosyringone. proven a better strain over the all tested factors. Putative transgenic roots were confirmed...
A Gram-staining negative, reddish-pink, non-motile, rod-shaped bacterial strain designated W29T, was isolated from a hexachlorocyclohexane-contaminated dumpsite located in the northern part of India at Ummari Village, Lucknow. Phylogenetic analysis based on 16S rRNA gene sequences showed that W29T formed lineage within genus Algoriphagus and exhibited highest sequence similarity to trabzonensis MS7T (98.8 %), followed by alkaliphilus AC-74T (97.1 %). The between other species ranged...
A novel orange to pink coloured bacterial strain designated as CT19 T was isolated from the gastrointestinal tract of mirror carp, Cyprinus carpio var. specularis (Lacepède, 1803) collected Gobind Sagar reservoir at village Lathiani, Una, Himachal Pradesh, India. Cells were found be aerobic, Gram-stain-positive, non-motile and non-spore-forming coccoids. Based on 16S rRNA gene sequence, closely related Salinicoccus hispanicus J-82 (=DSM 5352 ; 97.4 %), followed by S. sesuvii CC-SPL15-2 23267...
A Gram-staining-negative, red-pigmented, motile, rod-shaped bacterial strain, designated as W14T, was isolated from a hexachlorocyclohexane-contaminated dumpsite located in the northern part of India at Ummari Village, Lucknow. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain belongs to genus Pontibacter with highest similarity lucknowensis DM9T (98.1 %). The between W14T and members other species ranged 98.1 94.2 %. DNA-DNA relatedness P. 33.7 % closely related...
Multivariate analysis was carried out with 22 agro-morphological traits in 92 germplasm accessions of toria [Brassica rapa (L.) var. toria]. Principal factor identified eight principal components which explained about 62% variability. PC 1 had 15.04% total variation traits; 2 depicted 8.55% morphological variability, while 3 accounted for 7.68% the variation. Varimax rotation enabled loading similar type variables on a common permitting to designate them as seed yield, maturity, leaf and...
Here, we present the draft genome sequence of Deinococcus sp. strain S9, a red-pigmented and moderately thermophilic bacterium isolated from microbial mat deposits around hot springs at Manikaran, Himachal Pradesh, India. The (3.34 Mb) contains 101 contigs with an average GC content 66.4%.
Background: Six representative ITS1-PCR amplified products were used for the amplifications of conserved regions 18S and 5.8S rDNA to amplify Internal Transcribed Spacer1 (ITS1) gene 540 bp Typanosoma evansi isolates in equine from three agro-climatic zones Eastern Uttar Pradesh, India. Methods: Multiple sequence alignment was done by MAFFT online multiple tools selecting necessary parameters. The phylogenetic study ITS1 revealed all (T. horse India Pradesh- North Plane Zone, T. mule Zone...