A5040300433- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Cancer Research and Treatments
- Viral gastroenteritis research and epidemiology
- CRISPR and Genetic Engineering
Texas A&M University
2015-2021
New York University
2021
University of South Alabama
2016
Abstract Clostridium difficile is a significant concern as nosocomial pathogen, and genetic tools are important when analyzing the physiology of such organisms so that underlying physiology/pathogenesis can be studied. Here, we used TargeTron to investigate role selenoproteins in C . Stickland metabolism found insertion into selD , encoding selenophosphate synthetase essential for specific incorporation selenium selenoproteins, results growth defect global loss incorporation. However,...
ABSTRACT Clostridium difficile spore germination is essential for colonization and disease. The signals that initiate C. are a combination of taurocholic acid (a bile acid) glycine. Interestingly, the chenodeoxycholic class (CDCA) acids competitively inhibit acid-mediated germination, suggesting compounds could be developed into drugs prophylactically prevent infection or reduce recurring However, recent report called question utility such strategy to by describing strains germinated in...
Clostridium difficile is an important nosocomial pathogen and the leading cause of hospital-acquired diarrhea. Antibiotic use primary risk factor for development C. difficile-associated disease because it disrupts normally protective gut flora enables to colonize colon. damages host tissue by secreting toxins disseminates forming spores. The toxin-encoding genes, tcdA tcdB, are part a pathogenicity locus, which also includes tcdR gene that codes TcdR, alternate sigma initiates transcription...
Small RNAs (sRNAs) are short (∼50-200 nucleotides) noncoding that regulate cellular activities across bacteria. Salmonella enterica starved of a carbon-energy (C) source experience host genetic and physiological changes broadly referred to as the starvation-stress response (SSR). In an attempt identify novel sRNAs contributing SSR control, we grew log-phase, 5-h C-starved 24-h cultures virulent subspecies serovar Typhimurium strain SL1344 comprehensively sequenced their small RNA...
C. difficile is a Gram-positive, anaerobic gut pathogen which infects thousands of individuals each year. In order to stop the life cycle, other nonantibiotic treatment options are in urgent need development.
Abstract The endospore-forming pathogen, Clostridioides difficile , is the leading cause of antibiotic-associated diarrhea and a significant burden on community healthcare. C. like all forms life, incorporates selenium into proteins through selenocysteine synthesis pathway. known selenoproteins in are involved metabolic process that uses amino acids as sole carbon nitrogen source (Stickland metabolism). Stickland pathway requires use two selenium-containing reductases. In this study, we...