A5090433202- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Salmonella and Campylobacter epidemiology
- DNA and Nucleic Acid Chemistry
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Inflammatory mediators and NSAID effects
- interferon and immune responses
- Cancer Research and Treatments
- Plant and fungal interactions
- Bacteriophages and microbial interactions
- Species Distribution and Climate Change
- Pharmacogenetics and Drug Metabolism
- Immunotherapy and Immune Responses
- Acute Ischemic Stroke Management
- Cancer Immunotherapy and Biomarkers
- Protein Kinase Regulation and GTPase Signaling
- Bacterial Genetics and Biotechnology
- Glycosylation and Glycoproteins Research
- PI3K/AKT/mTOR signaling in cancer
- Estrogen and related hormone effects
- Immune cells in cancer
University of South Alabama
2016-2024
USA Mitchell Cancer Institute
2020-2022
University of Tennessee at Knoxville
1995
Genetic searches for tumor suppressors have recently linked small nucleolar RNA misregulations with tumorigenesis. In addition to their classically defined functions, several RNAs are now known be processed into short microRNA-like fragments called RNA-derived RNAs. To determine if any contribute breast malignancy, we performed a RNA-seq-based comparison of the two cancer cell lines (MCF-7 and MDA-MB-231) identified derived from 13 overexpressed in MDA-MB-231s. Importantly, find that...
Abstract Small mammal populations were characterized nine times during six sampling periods on plots in five different fields of tall fescue ( Festuca arundinacea Schreb.) to determine differences their abundance and species composition associated with the presence or absence endophytic fungus Acremonium coenophialum Morgan‐Jones & Gams. No found richness n = 4 for both types) between endophyte‐free (E − ) endophyte‐infested + plots. Capture success, number individuals, total captures,...
Small RNAs (sRNAs) are short (∼50-200 nucleotides) noncoding that regulate cellular activities across bacteria. Salmonella enterica starved of a carbon-energy (C) source experience host genetic and physiological changes broadly referred to as the starvation-stress response (SSR). In an attempt identify novel sRNAs contributing SSR control, we grew log-phase, 5-h C-starved 24-h cultures virulent subspecies serovar Typhimurium strain SL1344 comprehensively sequenced their small RNA...
We have identified 38 specifically excised, differentially expressed snoRNA fragments (sdRNAs) in TCGA prostate cancer (PCa) patient samples as compared to normal controls. SnoRNA-derived sdRNA-D19b and -A24 emerged among the most were selected for further experimentation. found that overexpression of either sdRNA significantly increased PC3 (a well-established model castration-resistant (CRPC)) cell proliferation, also markedly rate migration. In addition, both sdRNAs provided drug-specific...
Abstract Approximately 30% of human cancers harbor a gain‐in‐function mutation in the RAS gene , resulting constitutive activation protein to stimulate downstream signaling, including RAS‐mitogen activated kinase pathway that drives cancer cells proliferate and metastasize. RAS‐driven oncogenesis also promotes immune evasion by increasing expression programmed cell death ligand‐1, reducing major histocompatibility complex molecules present antigens T‐lymphocytes altering cytokines promote...
In the past decade, RNA fragments derived from full length small nucleolar RNAs (snoRNAs) have been shown to be specifically excised and functional. These sno-derived (sdRNAs) implicated as gene regulators in a multitude of cancers, controlling variety genes post-transcriptionally via association with RNA-induced silencing complex (RISC). this review, we summarized literature connecting sdRNAs cancer regulation. SdRNAs possess miRNA-like functions, are able fill role tumor-suppressor or...
Approximately 28 million people in the United States have precancerous colonic adenomas with many at high risk of developing colorectal cancer who could benefit from a pharmacological approach to prevent malignant progression. Clinical, epidemiological, and preclinical studies reported or provided mechanistic evidence that nonsteroidal anti-inflammatory drugs (NSAIDs) can reduce adenoma formation cancer. Unfortunately, long-term use NSAIDs is not recommended because potentially fatal...
Enhancers are genomic sequences that function as regulatory elements capable of increasing the transcription a given gene often located at considerable distance. The broadly accepted model enhancer activation involves bringing an enhancer-bound activator protein complex into close spatial proximity to its target promoter through chromatin looping. Equally relevant work described herein, roles for guanine (G) rich in transcriptional regulation now widely accepted. Non-coding G-rich commonly...
Several studies have now described instances where G-rich sequences in promoters and enhancers regulate gene expression through forming G-quadruplex (G4) structures. Relatedly, our group recently identified 301 long genomic stretches significantly enriched for minimal G4 motifs (LG4s) humans found the majority of these overlap annotated enhancers, furthermore, that regulated by LG4 are similarly with G4-capable sequences. While generally accepted model enhancer:promoter specificity maintains...
Abstract Salmonella Outer Membrane Vesicles (OMVs) were recently shown to inhibit P22 bacteriophage infection. Interestingly, we identify 31 recurrent tRFs abundantly expressed by enterica serovar Typhimurium and find these are highly complementary known -infecting (17 averaging 97.4% complementarity over 22.9 nt) specifically enriched in S. OMVs. Most notably, tRNA-Thr-CGT-1-1, 44-73, bears 100% its entire 30 nt length 29 distinct including P22. Importantly, inhibiting this tRF secreted...
ABSTRACT We have identified 38 specifically excised, differentially expressed snoRNA fragments (sdRNAs) in TCGA prostate cancer (PCa) patient samples as compared to normal controls. SnoRNA-derived sdRNA-D19b and -A24 emerged among the most were selected for further experimentation. found that overexpression of either sdRNA significantly increased PC3 (a well-established model castration-resistant (CRPC)) cell proliferation, also markedly rate migration. In addition, both sdRNAs provided...
Purpose of study: Having showed that polyclonal rabbit anti-CD83 antibodies, targeting activated dendritic cells (DC), induced T cell sparing immunosuppression in mouse and human preclinical models that, CMRF-44+ DC CCR5+ CD16+DC predicted for acute graft versus host disease (GVHD) after clinical allogeneic haematopoietic transplant, we developed a monoclonal antibody (mAb) as novel therapeutic. Methods: A scFv (3C12) specific CD83 was isolated from phage library. Affinity maturation...
ABSTRACT Salmonella Outer Membrane Vesicles (OMVs) were recently shown to inhibit P22 bacteriophage infection. Furthermore, despite there being several published reports now independently describing (1) the marked prevalence of tRFs within secreted vesicle transcriptomes and (2) roles for specific in facilitating/inhibiting viral replication, have been no examinations effects vesicle-secreted on infection reported date. Notably, while a number bacteria, expressed by salmonellae not...