A5057613879- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Chromosomal and Genetic Variations
- Plant nutrient uptake and metabolism
- Cancer Research and Treatments
- RNA Research and Splicing
- MicroRNA in disease regulation
- Plant Genetic and Mutation Studies
- Bacterial Genetics and Biotechnology
- Congenital heart defects research
- Viral Infectious Diseases and Gene Expression in Insects
- Potato Plant Research
- Aquaculture disease management and microbiota
- Plant Physiology and Cultivation Studies
- Cassava research and cyanide
- Computational Drug Discovery Methods
- Cancer-related molecular mechanisms research
- Plant Disease Resistance and Genetics
- Photonic and Optical Devices
East Georgia State College
2015-2024
Mississippi State University
2008-2021
University of Zurich
1999-2017
University of Missouri
2005-2008
Technical University of Munich
2005-2007
Département Santé des Plantes et Environnement
1988
Abscission is a developmental program that results in the active shedding of infected or nonfunctional organs from plant body. Here, we establish signaling pathway controls abscission Arabidopsis thaliana ligand, to receptors, downstream effectors. Loss function mutations Inflorescence Deficient (IDA), which encodes predicted secreted small protein, receptor-like protein kinases HAESA (HAE) and HAESA-like 2 (HSL2), Mitogen-Activated Protein Kinase 4 (MKK4) MKK5, dominant-negative form 6...
Proteins containing the forkhead-associated domain (FHA) are known to act in biological processes such as DNA damage repair, protein degradation, and signal transduction. Here we report that DAWDLE (DDL), an FHA domain-containing Arabidopsis, acts biogenesis of miRNAs endogenous siRNAs. Unlike mutants genes participate processing miRNA precursors, dcl1, hyponastic leaves1, serrate, ddl show reduced levels pri-miRNAs well mature miRNAs. Promoter activity MIR genes, however, is not affected by...
Sexual reproduction is a salient aspect of plants, and elaborate structures, such as the flowers angiosperms, have evolved that aid in this process. Within flower corresponding sex organs, anther ovule, form male female sporangia, pollen sac nucellus, respectively. However, despite their central role for sexual little known about mechanisms control establishment these important structures. Here we present identification molecular characterization NOZZLE (NZZ) gene flowering plant Arabidopsis...
Our objectives were to identify substances produced by plant roots that might act as nutritional mediators of specific plant-bacterium relationships and delineate the bacterial genes responsible for catabolizing these substances. We discovered new compounds, which we call calystegins, have characteristics mediators. They detected in only 3 105 species higher plants examined: Calystegia sepium, Convolvulus arvensis (both Convolvulaceae family), Atropa belladonna. Calystegins are abundant...
An open question remains as to what coordinates cell behavior during organogenesis, permitting organs reach their appropriate size and shape. The Arabidopsis gene STRUBBELIG ( SUB ) defines a receptor-mediated signaling pathway in plants. encodes putative leucine-rich repeat transmembrane receptor-like kinase. mutant sub phenotype suggests that affects the formation shape of several by influencing morphogenesis, orientation division plane, proliferation. Mutational analysis kinase domain is...
Calcium-dependent protein kinase (CDPK)-SNF1related
Abstract Background Receptor-like kinases are a prominent class of surface receptors that regulate many aspects the plant life cycle. Despite recent advances function most receptor-like remains elusive. Therefore, it is paramount to investigate these receptors. The task complicated by fact belong large monophyletic family with sub-clades. In general, functional analysis gene members reverse genetics often obscured several issues, such as redundancy, subtle or difficult detect phenotypes in...
Abstract Phosphoprotein-binding domains are found in many different proteins and specify protein-protein interactions critical for signal transduction pathways. Forkhead-associated (FHA) bind phosphothreonine control aspects of cell proliferation yeast (Saccharomyces cerevisiae) animal cells. The Arabidopsis (Arabidopsis thaliana) protein kinase-associated phosphatase includes a FHA domain that mediates with receptor-like kinases, which turn regulate variety signaling pathways involved plant...
Small RNAs (sRNAs) are short (∼50-200 nucleotides) noncoding that regulate cellular activities across bacteria. Salmonella enterica starved of a carbon-energy (C) source experience host genetic and physiological changes broadly referred to as the starvation-stress response (SSR). In an attempt identify novel sRNAs contributing SSR control, we grew log-phase, 5-h C-starved 24-h cultures virulent subspecies serovar Typhimurium strain SL1344 comprehensively sequenced their small RNA...
We report a new type of refractive index-based biosensor using fiber loop ringdown evanescent field (FLRD-EF) sensing scheme, in which the signal is time constant and detection sensitivity enhanced by multipass nature technique. Bulk detections three different single strand DNAs one bacteria are demonstrated for FLRD-EF sensors that utilize partially-etched mode as sensor head. Stepwise coating head with poly-L-lysine probe DNA has enabled surface label-free target sensing. expect an array...
DAWDLE (DDL) is a conserved forkhead-associated (FHA) domain-containing protein with essential roles in plant development and immunity. It acts the biogenesis of microRNAs (miRNAs) endogenous small interfering RNAs (siRNAs), which regulate gene expression at transcriptional and/or posttranscriptional levels. However, functional mechanism DDL its impact on exogenous siRNAs remain elusive. Here, we report that required for derived from sense transgenes inverted-repeat transgenes. Furthermore,...
DAWDLE (DDL) gene encodes a protein that contains an N-terminal arginine-rich domain and C-terminal Fork Head Associated (FHA) in Arabidopsis thaliana. DDL is believed to function microRNA biogenesis by mediating the recruitment of pri-microRNA DICER-LIKE 1 also stabilizing microRNA. The aim this study was conduct structure-function analysis identify regions are functional significance. Targeted Induced Local Lesions Genome screen performed Columbia erecta-105 background resulting...
The rapid developments in high-throughput sequencing technologies have allowed researchers to analyze the full genomic sequence of organisms faster and cheaper than ever before. An important application such advancements is identify impact single nucleotide polymorphisms (SNPs) on phenotypes genotypes same species by discovering factors that affect occurrence SNPs. focus this study determine whether climate as main climate, precipitation, temperature affecting a certain geographical area...
The screening analysis of loss-of-function alleles in Arabidopsis thaliana revealed a mutation the At3G20550 gene, called DAWDLE (DDL). DDL gene causes pleiotropic phenotypes and reduced levels several microRNAs. encodes protein with Fork Head-Associated (FHA) domain, found large range proteins significant cellular processes prokaryotes eukaryotes. However, it is not completely known whether FHA domain C-terminal region are necessary for its function. aim this study was to determine function...
DDL encodes a protein bearing an FHA domain in Arabidopsis thaliana. is phospho‐threonine binding plant and animal proteins that function DNA repair cell cycle regulation. N‐terminal region of displays sequence homology with involved RNA processing. can bind to microRNA vitro. SMAD nuclear interacting 1, ortholog human, regulates stability Cyclin D1. We propose processing genes regulating aim elucidate the potential role its signaling pathway by genetic approach. T‐DNA insertion knockout...