A5034365881- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Asthma and respiratory diseases
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Gut microbiota and health
- Birth, Development, and Health
- Clostridium difficile and Clostridium perfringens research
- Neonatal Respiratory Health Research
- Stress Responses and Cortisol
- Health, psychology, and well-being
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Health, Environment, Cognitive Aging
- Sex and Gender in Healthcare
- Health disparities and outcomes
- Herpesvirus Infections and Treatments
- Diabetes and associated disorders
- Dietary Effects on Health
- Cardiovascular Health and Risk Factors
- Pancreatic function and diabetes
- Human Health and Disease
- Nutrition, Genetics, and Disease
- Endoplasmic Reticulum Stress and Disease
Wayne State University
2020-2025
University of Florida
2016
Synthetic glucocorticoids, such as dexamethasone, have been used a treatment for many immune conditions, asthma and, more recently, severe COVID-19. Single-cell data can capture fine-grained details on transcriptional variability and dynamics to gain better understanding of the molecular underpinnings inter-individual variation in drug response. Here, we single-cell RNA-seq study response glucocorticoids activated peripheral blood mononuclear cells from 96 African American children. We novel...
Social factors influence health outcomes and life expectancy. Individuals living in poverty often have adverse related to chronic inflammation that affect the cardiovascular, renal, pulmonary systems. Negative psychosocial experiences are associated with transcriptional changes genes complex traits. However, underlying molecular mechanisms by which increases risk of disease disparities still not fully understood. To bridge gap our understanding link between outcomes, we performed RNA...
ABSTRACT We previously reported that an amino acid substitution, Y704A, near the 2-fold interface of adeno-associated virus (AAV) was defective for transcription packaged genome (M. Salganik, F. Aydemir, H. J. Nam, R. McKenna, M. Agbandje-McKenna, and N. Muzyczka, J Virol 88:1071–1079, 2013, doi: http://dx.doi.org/10.1128/JVI.02093-13 ). In this report, we have characterized defect in 6 additional capsid mutants located a region ∼30 Å diameter on surface AAV type 2 (AAV2) interface. These...
Abstract Puberty is an important developmental period marked by hormonal, metabolic and immune changes. also marks a shift in sex differences susceptibility to asthma. Yet, little known about the gene expression changes cells that occur during pubertal development. Here we assess development leukocyte longitudinal cohort of 251 children with We identify substantial associated age Gene between pre- post-menarcheal females suggest from predominantly innate adaptive immunity. show genetic...
Social interactions and the overall psychosocial environment have a demonstrated impact on health, particularly for people living in disadvantaged urban areas. Here, we investigated effect of experiences gene expression peripheral blood immune cells children with asthma Metro Detroit. Using RNA-sequencing new machine learning approach, identified transcriptional signatures 19 variables including factors, cell composition, symptoms. Importantly, found 169 genes associated or allergic disease...
The gut microbiome exhibits extreme compositional variation between hominid hosts. However, it is unclear how this impacts host physiology across species and whether effect can be mediated through microbial regulation of gene expression in interacting epithelial cells. Here, we characterize the transcriptional response human colonic cells vitro to live communities extracted from humans, chimpanzees, gorillas, orangutans. We find that most genes exhibit a conserved response, whereby they...
Synthetic glucocorticoids, such as dexamethasone, have been used treatment for many immune conditions, asthma and more recently severe COVID-19. Single cell data can capture fine-grained details on transcriptional variability dynamics to gain a better understanding of the molecular underpinnings inter-individual variation in drug response. Here, we single RNA-seq study response glucocorticoids activated Peripheral Blood Mononuclear Cells from 96 African American children. We employed novel...
Abstract To better understand COVID-19 pathobiology and to prioritize treatment targets, we sought identify human genes influencing genetically driven disease risk severity, additional organismal-level phenotypes impacted by pleiotropic COVID-19-associated genomic loci. this end, performed ancestry-aware, trans-layer, multi-omic analyses integrating recent Host Genetics Initiative genome-wide association (GWAS) data from six ancestry endpoints - African, Amerindian, South Asian, East...
Abstract Cis-regulatory elements (CREs) control gene transcription dynamics across cell types and in response to the environment. In asthma, multiple immune play an important role inflammatory process. Genetic variants CREs can also affect expression contribute asthma risk. However, regulatory mechanisms underlying of transcriptional different environmental contexts cell-types at single resolution remains be elucidated. To resolve this question, we performed scATAC-seq activated peripheral...
Abstract Social interactions and the overall psychosocial environment have a demonstrated impact on health, particularly for people living in disadvantaged urban areas. Here we investigated effect of experiences gene expression peripheral blood immune cells children with asthma Metro Detroit. Using RNA-sequencing new machine learning approach, identified transcriptional signatures 20 variables including factors, cell composition symptoms. Importantly, found 174 genes associated that are...
Abstract The gut microbiome exhibits extreme compositional variation between hominid hosts. However, it is unclear how this impacts host physiology, and whether effect can be mediated through microbial regulation of gene expression in interacting epithelial cells. Here, we characterized the transcriptional response colonic cells vitro to live communities extracted from humans, chimpanzees, gorillas, orangutans. We found most genes exhibit a conserved response, whereby they respond similarly...
The gut microbiome exhibits extreme compositional variation between hominid hosts. However, it is unclear how this impacts host physiology, and whether effect can be mediated through microbial regulation of gene expression in interacting epithelial cells. Here, we characterized the transcriptional response colonic cells vitro to live communities extracted from humans, chimpanzees, gorillas, orangutans. We found most genes exhibit a conserved response, whereby they respond similarly four...
Abstract Social factors influence health outcomes and life expectancy. Individuals living in poverty often have adverse related to chronic inflammation that affect the cardiovascular, renal, pulmonary systems. Negative psychosocial experiences are associated with transcriptional changes genes complex traits. However, underlying molecular mechanisms by which increases risk of disease disparities still not fully understood. To bridge gap our understanding link between outcomes, we performed...
Abstract Puberty is an important developmental period marked by hormonal, metabolic and immune changes. also marks a shift in sex differences susceptibility to asthma. Yet, little known about the gene expression changes cells that occur during pubertal development. Here we assess development leukocyte longitudinal cohort of 251 children with We identify substantial associated age Gene between pre- post-menarcheal females suggest from predominantly innate adaptive immunity. show genetic...