The systematic investigation of the interaction a new class molecular materials with proteins through structure-optical signaling relationship studies has led to development efficient fluorescent probes that can detect and quantify serum albumins in biofluids without causing any denaturation.

Cysteine cathepsins are important proteases that highly upregulated in cancers and other diseases. While their reported location is mostly endolysosomal, some evidence shows nuclear localization involvement the cell cycle. We aim to generate tools investigate of cycle progression. To cathepsin activity, we designed nucleus-directed quenched activity-based probes (qABPs) by attaching cell-penetrating peptides (CPPs). qABPs active-site-directed compounds enable direct real-time monitoring...

A very aggressive and deadly brain cancer, glioblastoma multiforme (GBM) poses formidable obstacles to effective therapy. Despite advancements in conventional therapies like surgery, chemotherapy, radiation therapy, the prognosis for GBM patients remains poor, with limited survival outcomes. Nanotechnology is gaining popularity as a promising platform managing GBM, offering targeted drug delivery, improved therapeutic efficacy, reduced systemic toxicity. This review offers comprehensive...

Cathepsin K (CTSK), a proteolytic enzyme that degrades the extracellular matrix, is recognized as significant therapeutic target for osteoporosis, osteoarthritis, and rheumatoid arthritis. Due to adverse effects, no clinically approved drugs exist CTSK. In order develop safer therapeutics, highly selective CTSK inhibitors are required elucidate origins of side effects. Here, we developed various hybrid by combining peptide sequences with small organic molecules. An acyloxymethyl ketone...

Development of a highly photostable, renal clearable, and nontoxic new NIR probe (CyG) for precise quantification albumin in different biofluids liver targeted vivo visualization is demonstrated. CyG's inherent property to interact selectively with among biomolecules intracellular environment high degree sensitivity helps CyG imaging. In addition its long excitation/emission wavelengths (λex = 740 nm, λem 804 nm), which are much above the biological tissue opaque window (400-700 nm) ensuring...

Five new, star-shaped compounds containing a central oligo(phenylenevinylene) core carrying six alkoxy chains were synthesized, and with varying alkyl the changes in their supramolecular assembly explored by small wide-angle X-ray scattering (SAXS/WAXS) studies. For shortest peripheral chain (3.1; R = 3,7-dimethyl octyl) lamellar stacking was favored, whereas increasing length (3.2, 3.3, 3.4, 3.5; n-C12H25, n-C14H29, n-C16H33, n-C18H35) columnar rectangular (Colr) observed. The compound...

Cathepsin-K (CTSK) is an osteoclast-secreted cysteine protease that efficiently cleaves extracellular matrices and promotes bone homeostasis remodeling, making it excellent therapeutic target. Detection of CTSK activity in complex biological samples using tailored tools such as activity-based probes (ABPs) will aid tremendously drug development. Here, potent selective are designed created, comparing irreversible reversible covalent ABPs with improved recognition components electrophiles. The...

The work describes an integrated optical platform for recognition of Cr3+ at the picomolar level (0.66 pM). condensation a fluorene unit with l-leucine led to development highly fluorescent molecular probe L which detects Cu2+ following turn-off signaling mechanism. Further, L-Cu2+ ensemble has been successfully utilized as light-up tool selective turn-on sensing Cr3+, first time, through quencher displacement. This process eventually selectively one paramagnetic cation by displacement...

Interesting self-assembly of an amino acid based molecular material into a hydrogel in the presence selective enantiomeric chiral amines leading to recognition has been demonstrated. Moreover, collapse metallogel formed from same enantiomers validated its enantioselective affinity. Importantly, addition relevant experimental techniques, DFT studies have successfully explored establish through gelation.

Selective staining of human serum albumin protein in gel electrophoresis over wide range other protein(s) is extremely important because it contains more than 60% volume fluid body. Given the nonexistence suitable dye materials for selective albumins electrophoresis, we report a new class easy synthesizable and low molecular weight agents based on 3-amino-N-alkyl-carbazole scaffold solid phase. A detailed structure–efficiency relationship (SER) study enabled us to develop two such potent...

Structure-interaction/fluorescence relationship studies led to the development of a small chemical library Zn2+-specific cysteamine-based molecular probes. The probe L5 with higher excitation/emission wavelengths, which absorbs in visible region and emits green, was chosen as model imaging material for biological studies. After successful intracellular zinc four different kinds cells including living organisms, plant, animal cells, vivo potential evaluated using plant systems. In...

Successful integration of selenium unit into a newly designed cationic chemical architecture led to the development highly photostable molecular maker PA5 be used in fluorescence microscopy as cellular nucleus staining agent for longer duration imaging under continuous laser illumination. Adaptation targeted single-atom modification strategy series proficient DNA light-up probes (PA1–PA5). Further, their comparative photophysical studies presence revealed potential electron rich heteroatoms...

With the promising advantages of near-infrared region (NIR) emissive markers for serum albumin becoming very prominent recently, we devised CyG-NHS as cyanine derived longest NIR-I optical marker possessing selective recognition ability in diverse biological milieu. Multiscale modeling involving molecular docking, dynamics, and implicit solvent binding free energy calculations have been employed to gain insights into unique developed probe at domain-I albumin, contrast good number domain IIA...

Albumin is the most abundant serum protein and shows variation in its synthesis rate different physiological pathophysiological conditions. Thus, there might be an association expected between albumin concentration body health. A library of NIR probes engineered with optimum hydrophobicity has been developed characterized using spectroscopy techniques was employed to understand hepatic rates on states. Given importance rendering effective interaction small molecules biomolecules, strategic...

Social science NLP tasks, such as emotion or humor detection, are required to capture the semantics along with implicit pragmatics from text, often limited amounts of training data. Instruction tuning has been shown improve many capabilities large language models (LLMs) commonsense reasoning, reading comprehension, and computer programming. However, little is known about effectiveness instruction on social domain where pragmatic cues needed be captured. We explore use for tasks introduce...

The space environment can affect the function of all physiological systems, including properties cell membranes. Our goal in this study was to explore effect simulated microgravity (SMG) on cellular uptake small molecules based reported microgravity-induced changes membrane properties. SMG applied cultured cells using a random-positioning machine for up three hours. We assessed accumulation compounds representing substrates and efflux transporters, not shown be transported by carriers....

As the postsynthesis-processed metal-organic material-based catalysts for energy applications add additional cost to whole process, importance of developing synthesized usable pristine is quite evident. The present work reports a new Cu-based coordination polymer (Cu-CP) catalyst be used in its form oxygen reduction reaction (ORR) application. was characterized using single-crystal X-ray diffraction, field emission scanning electron microscopy, and photoemission spectroscopy. Cu-CP exhibits...

In this paper we have proposed a fluorescence based spectroscopy device which can be used to quantitatively estimate the amount of albumin that gets excreted out our body. Albumin is significant protein in bio-fluids and performs wide range metabolic functions. The dye has been as fluorescent indicator for presence study earlier tested with bovine serum (BSA) human (HSA) satisfactory results. method on principle near infrared (NIR) 700 850 nm by using novel test mixture. chosen benefit...

<p>For the understanding of deformational mechanism and geodynamics a tectonic set up, source localization central depth anisotropy plays vital role. Though mantle dynamics deformation patterns can be understood from studying shear wave splitting mechanism, true interpretation under earth governing remains little biased unrealistic without the  proper justification identification anisotropy. Our present study is focused on possible determination beneath Sikkim...