A5006457959- Immunotherapy and Immune Responses
- Protease and Inhibitor Mechanisms
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- S100 Proteins and Annexins
- Diabetes and associated disorders
- Calpain Protease Function and Regulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Peptidase Inhibition and Analysis
- Antimicrobial Peptides and Activities
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- Glioma Diagnosis and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Extracellular vesicles in disease
- Biochemical and Structural Characterization
- Infant Nutrition and Health
- interferon and immune responses
- Bone Metabolism and Diseases
Nazarbayev University
2018-2025
Universität Ulm
2008-2018
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
2014-2016
Polish Academy of Sciences
2014-2016
University Hospital Ulm
2016
University of Gdańsk
2012
University of Tübingen
2002-2012
Stanford University
2005-2007
Pediatrics and Genetics
2006
Glioblastoma multiforme (GBM), a common primary malignant brain tumor, rarely disseminates beyond the central nervous system and has very bad prognosis. The current study aimed at analysis of immunological control in individual patients with GBM.Immune phenotypes plasma biomarkers GBM were determined time diagnosis using flow cytometry ELISA, respectively.Using descriptive statistics, we found that immune anomalies distinct patients. Defined marker profiles proved highly relevant for...
The success of DNA analytical methods, including long-read sequencing, depends on the availability high-quality, purified DNA. Previously, we developed a method and device for isolating high-molecular-weight (HMW) sequencing using high-salt gel electroelution trap. Here, present an improved version this purifying nucleic acids with high yield purity from even most challenging biological samples. proposed is significant improvement over previously published procedure, offering simple, fast,...
SARS-CoV-2, the pathogenic virus that induces COVID-19 disease, contains four structural proteins in its virion. The nucleocapsid (N) protein is one of play a crucial role assembly viral RNA into ribonucleoprotein. In addition, N contributes to pathogenesis. One functions attributed triggering cytokine release by lung epithelial cells, macrophages, and monocytes. This study addresses cellular effects SARS-CoV-2 on cells glial origin. We report upregulation RANTES chemokine A172 glioblastoma...
Abstract The asparagine-specific endoprotease (AEP) controls lysosomal processing of the potential autoantigen myelin basic protein (MBP) by human B lymphoblastoid cells, a feature implicated in immunopathogenesis multiple sclerosis. In this study, we demonstrate that freshly isolated lymphocytes lack significant AEP activity and cleavage is dispensable for proteolytic MBP type cell. Instead, cathepsin (Cat) G, serine protease not endogenously synthesized lymphocytes, internalized from...
Serine proteases neutrophil elastase (NE), protease 3 (PR3), cathepsin G (CatG), and serine 4 (NSP4) are released by activated neutrophils swarming around the place of pathogen invasion to provoke an immune response. However, uncontrolled proteolytic activity results in various human diseases, including cardiovascular thrombosis, autoimmunity. In addition, can be hijacked several viruses prime virus-derived surface proteins evade detection entering into host cell. Indeed, porcine increases...
The serine proteases neutrophil elastase (NE), proteinase 3 (PR3), cathepsin G (CatG), and protease 4 (NSP4) are secreted by activated neutrophils as a part of the innate immune response against invading pathogens. However, these might be adopted viruses to mediate viral surface protein priming resulting in host cell entrance productive infection. Indeed, NE PR3 hydrolyze scissile peptide bond within proteolytically sensitive polybasic sequence activation loop SARS-CoV-2 located at S1/S2...
Abstract Dendritic cells (DC) initiate immunity and maintain tolerance. Although in vitro-generated DC, usually derived from peripheral blood monocytes (MO-DC), serve as prototype DC to analyze the biology biochemistry of phenotypically distinct primary types including CD1c-DC, are present (PB-DC). The composition lysosomal proteases PB-DC way their MHC class II-associated Ag-processing machinery handles a clinically relevant Ag unknown. We show that CD1c-DC lack significant amounts active...
Abstract Expression of HLA-DO (DO) in cells that express HLA-DM (DM) results an altered repertoire MHC class II/peptide complexes, indicating DO modulates DM function. Human and murine B thymic epithelial DO, while monocytes/macrophages do not. Monocyte-derived dendritic (DC) also have been found to be DO-negative, leading the assumption DC not DO. In this study, we report that, fact, certain types human primary These include Langerhans (LC) some subtypes circulating blood DC. Specifically,...
Protease-mediated degradation of proteins is critical in a plethora physiological processes. Neutrophils secrete serine proteases including cathepsin G (CatG), neutrophile elastase (NE), and proteinase 3 (PR3) together with lactoferrin (LF) as first cellular immune response against pathogens. Here, we demonstrate that LF increases the catalytic activity CatG at concentration, its highest enhancing capacity under acidic (pH 5.0) conditions, broadens substrate selectivity CatG. On functional...
Proteolysis by endocytic cysteine proteases is a central element of the antigen-presentation machinery in dendritic cells (DC). It controls generation immunogenic peptides, guides transit both MHC class II and MHC-like molecules through compartment converts into peptide-receptive state — features closely linked to DC maturation. Differential activity proteases, particular cathepsins, subcellular compartments has been implicated as key regulatory controlling this murine DC. We analyzed...
Autoantigenic peptides resulting from self-proteins such as proinsulin are important players in the development of type 1 diabetes mellitus (T1D). Self-proteins can be processed by cathepsins (Cats) within endocytic compartments and loaded to major histocompatibility complex (MHC) class II molecules for CD4(+) T cell inspection. However, processing presentation antigen-presenting cells (APC) humans is only partially understood. Here we demonstrate that B or myeloid dendritic (mDC1)-derived...