A5006965606- Peptidase Inhibition and Analysis
- Protease and Inhibitor Mechanisms
- Animal health and immunology
- Monoclonal and Polyclonal Antibodies Research
- Neuropeptides and Animal Physiology
- Herpesvirus Infections and Treatments
- Cell Adhesion Molecules Research
- Virology and Viral Diseases
- Click Chemistry and Applications
- Toxin Mechanisms and Immunotoxins
- Synthesis and biological activity
- Studies on Chitinases and Chitosanases
- Pneumocystis jirovecii pneumonia detection and treatment
- Carbohydrate Chemistry and Synthesis
- Mosquito-borne diseases and control
- RNA Interference and Gene Delivery
- Neuroendocrine Tumor Research Advances
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Genomics, phytochemicals, and oxidative stress
- S100 Proteins and Annexins
- Adenosine and Purinergic Signaling
- Glutathione Transferases and Polymorphisms
- Cell death mechanisms and regulation
- Systemic Sclerosis and Related Diseases
Wrocław University of Science and Technology
2014-2023
AGH University of Krakow
2016-2023
The O'nyong‐nyong virus (ONNV) is a member of the Alphavirus genus isolated for first time in 1959 Gulu (Uganda). main symptoms fever are nonspecific and flu‐like; proper diagnosis at early stage challenging. Until today, ONNV epidemic 1959–1962 remains one largest arbovirus outbreaks ever recorded (over 2 million cases). No specific therapy available thus far. Capsid protease (CP) chymotrypsin‐like serine that catalyzes autoproteolytic maturation structural polyprotein undergoes...
Early detection of cancer development is crucial for successful therapy and monitoring patient outcome. Various immunodiagnostic methods are able to detect pathological changes in the human body ahead symptomatic manifestation disease. Most immunological examinations based on specific tumor markers fluids. Of various cancer-specific proteins used breast diagnostics, one most commonly applied antigen 15-3 (CA 15-3). An elevation its serum level (>25-40 U/ml) usually correlates with...
Aminopeptidase N/CD13 (EC 3.4.11.2) is suggested to play a role in cancer cells invasion, and its activity can be inhibited using specific inhibitors. CD13 inhibitors evoke apoptosis of CD13-positive cells. However, expression has not been described specimens obtained from ovarian carcinomas. Thus, the present study, significance was examined samples cancers. The analyses were performed on sections originating 73 tumor (43 primary laparotomies [PL] 30 secondary cytoreductions [SCRs])....
Herpes simplex virus type 1 (HSV-1) is a widespread human pathogen known to cause infections of diverse severity, ranging from mild ulceration mucosal and dermal tissues life-threatening viral encephalitis. In most cases, standard treatment with acyclovir sufficient manage the disease progression. However, emergence ACV-resistant strains drives need for new therapeutics molecular targets. HSV-1 VP24 protease indispensable assembly mature virions and, as such, constitutes an interesting...
Measurement of serum prostate specific antigen (PSA) concentrations remains one the leading methods for diagnosing cancer. We developed and evaluated an immunoglobulin Y (IgY)-based ELISA to measure total PSA (tPSA) in human that could be used as alternative commercially available vitro diagnostic assays rely on mouse monoclonal IgG.A sandwich based anti-PSA IgY antibody was developed. ability detect free complexed at same molar ratio. The assay optimized, its analytical performance verified...
Abstract Neutrophils are a type of granulocyte important in the “first line defense” innate immune system. Upon activation, they facilitate destruction invading microorganisms by production superoxide radicals, as well release enzymatic contents their lysozymes. These enzymes include specific serine proteases: cathepsin G, neutrophil elastase, proteinase 3, recently discovered protease 4 (NSP4). Under normal conditions, proteolytic activity proteases is tightly regulated endogenous serpins;...
Bartosz Miziołekab* , Marcin Sieńczykc Renata Grzywac, Agnieszka Łupicka-Słowikc, Eugeniusz Kucharzb, Przemysław Kotylab & Beata Bergler-Czopa a Department of Dermatology, School Medicine in Katowice, Medical University Silesia, Polandb Internal Rheumatology and Clinical Immunology, Polandc Organic Medicinal Chemistry, Faculty Wroclaw Science Technology, Wrocław, Poland
The neutrophilic serine protease proteinase 3 (PR3) is involved in inflammation and immune response thus appears as a therapeutic target for variety of infectious inflammatory diseases. Here we combined kinetic molecular docking studies to increase the potency peptidyl-diphenyl phosphonate PR3 inhibitors. Occupancy S1 subsite by nVal residue S4–S5 subsites biotinylated Val obtained biotin-VYDnVP(O-C6H4-4-Cl)2 enhanced second-order inhibition constant kobs/[I] toward more than 10 times...
Background: The prostate-specific antigen (PSA) is considered an important serum marker for prostate cancer detection, monitoring and staging. purpose of this study was to generate IgY class antibodies that recognize native PSA selected epitopes. Methodology: Hens immunized with either full-length human or its peptidyl fragment-conjugates produced specific were isolated from egg yolks. We developed a monoclonal/IgY sandwich ELISA detection limit 50 pg/ml linear range 0.05–1.0 ng/ml....
Abstract Herein we present the synthesis of a novel type peptidomimetics composed repeating diaminopropionic acid residues modified with structurally diverse heterobifunctional polyethylene glycol chains (abbreviated as DAPEG). Based on developed compounds, library fluorogenic substrates was synthesized. Further deconvolution towards human neutrophil serine protease 4 (NSP4) yielded highly sensitive and selective internally quenched peptidomimetic substrates. In silico analysis obtained...
ABSTRACT The IgY antibodies offer an attractive alternative to mammalian IgGs in research, diagnosis and medicine. isolation of immunoglobulin Y from the egg yolks is efficient economical, causing minimal suffering animals. Here we present methodology for production specific Staphylococcus aureus fibrinogen binding protein (Efb) its peptidyl epitope (spanning residues 127–140). Efb extracellular, adhesion which binds both human complement C3 thus contributing high infectious potential this...
Adenosine deaminase (ADA) is currently used as a diagnostic marker for tuberculous pleuritis. Although ADA has been suggested potential several types of cancer, the importance each isoforms well their levels and enzymatic activities in tumors need to be further investigated. Herein we developed avian immunoglobulin Y highly specific human via hens immunization with calf adenosine deaminase. The obtained antibodies were development sensitive double-egg yolk (IgY) sandwich ELISA assay an...