A5001348096- Hepatitis C virus research
- Hepatitis B Virus Studies
- Viral gastroenteritis research and epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Transgenic Plants and Applications
- Streptococcal Infections and Treatments
- vaccines and immunoinformatics approaches
- Hepatitis Viruses Studies and Epidemiology
- Glycosylation and Glycoproteins Research
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Immunotherapy and Immune Responses
- Cervical Cancer and HPV Research
- Protease and Inhibitor Mechanisms
- HIV Research and Treatment
- Toxin Mechanisms and Immunotoxins
- Viral Infectious Diseases and Gene Expression in Insects
- Neonatal and Maternal Infections
- Immune Cell Function and Interaction
- Protein purification and stability
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Mosquito-borne diseases and control
- Microbial infections and disease research
Pasteur Institute of Iran
2016-2025
Islamic Azad University Tonekabon
2015
Centre National de la Recherche Scientifique
2011
Institut Pasteur
2004-2011
Early events leading to the establishment of hepatitis C virus (HCV) infection are not completely understood. We show that intact and dynamic microtubules play a key role in initiation productive HCV infection. Microtubules were required for entry into cells, as evidenced using pseudotypes presenting envelope proteins on their surface. Studies carried out recent infectious model revealed also an essential early, postfusion steps cycle. Moreover, low concentrations vinblastin nocodazol,...
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Abstract Lambda phages have considerable potential as gene delivery vehicles due to their genetic tractability, low cost, safety and physical characteristics in comparison other nanocarriers porters. Little is known concerning lambda phage-mediated transfer expression mammalian hosts. We therefore performed experiments evaluate lambda-ZAP bacteriophage-mediated vitro . For this purpose, we constructed recombinant λ-phage nanobioparticles containing a cassette encoding enhanced green...
Hepatitis C virus (HCV) infection is a major cause of chronic liver disease worldwide. HCV core protein involved in nucleocapsid formation, but it also interacts with multiple cytoplasmic and nuclear molecules plays crucial role the development hepatocarcinogenesis. The found mostly cytoplasm during infection, nucleus patients hepatocarcinoma core-transgenic mice. contains localization signals (NLS), no export signal (NES) has yet been identified.We show here that aa(109-133) region directs...
Plants transformed by virus-based vectors have emerged as promising tools to rapidly express large amounts and inexpensive antigens in transient condition.We studied the possibility of transient-expression an HBsAg-fused polytopic construct (HCVpc) [containing H-2d HLA-A2-restricted CD8+CTL-epitopic peptides C (Core; aa 132-142), E6 (Envelope2; 614-622), N (NS3; 1406-1415), E4 405-414) tandem CE6NE4] tobacco (Nicotiana tabacum) leaves for development a plant-based HCV vaccine.A...
We have previously demonstrated that viral particles with the properties of nonenveloped hepatitis C virus (HCV) nucleocapsids occur in serum HCV-infected individuals (1). show here purified directly from or isolated HCV virions FcγR-like activity and bind “nonimmune” IgG via its Fcγ domain. core proteins produced Escherichia coli baculovirus expression system also bound their fragments. Folded conformation was required for binding because site protein inactive denaturing conditions. Studies...
Abstract Persistent infection with high-risk genotypes of human papillomavirus (HPV) is the leading cause cervical cancer. The HPV oncoprotein E7 constitutively expressed in cancer and considered as an essential target for tumor-specific immunity. goal this study was to develop a candidate therapeutic vaccine based on mutated protein that had possibly reduced transformation capacity while able elicit robust immune response. Therefore, mutant type 16 (E7GRG) recombinantly E. coli . then...
An increasing attitude towards oncolytic viruses (OVs) is witnessed following T-VEC's approval. In this study, we aimed to delete ICP47 and insert IL-12 in the ICP34.5 deleted HSV-1 backbone improve properties provide an immune-stimulatory effect respectively. The wild-type recombinant infected both cancerous, SW480 HCT116, non-cancerous, HUVEC, cell lines. Green-red Δ47/Δ34.5 was constructed by replacing with GFP. Both copies were replaced hIL12. Cytotoxicity growth kinetics of...
For chronic viral infections like Hepatitis C, CD8-CTLs have emerged as important protective tools. Hence, isolated dominant epitopes arranged polytope DNA or peptide vaccines represent a promising approach. However, because of controversial rules governing the construction and epitope processing, proper design primary analysis such are prior to costly transgenic animal studies. In this study, based on in silico selection, four HLA-A2 (C132, E614 N1406) H-2d (E405 C132) immunodominant...
The development of an efficient vaccine against high-risk HPV types can reduce the incidence rates cervical cancer by generating anti-tumor protective responses. Traditionally, majority prophylactic viral vaccines are composed live, attenuated or inactivated viruses. Among them, design effective and low-cost is critical. Inactivated especially heat-killed yeast cells have emerged as a promising approach for antigen-specific immunotherapy. Recent studies indicated that cell wall components...
Application of streptokinase (SK) as a common and cost-effective thrombolytic drug is limited by its antigenicity undesired hemorrhagic effects. Prior structural/functional epitope-mapping studies on SK suggested that removal 59 N-terminal residues led to fibrin dependency identified antigenic regions, respectively. Following in silico analyses two truncated proteins were designed compared for their specificity with the full-length SK. Computer-based modeling was used predict effect vector...