A5055158419- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Virus-based gene therapy research
- Wnt/β-catenin signaling in development and cancer
- Genetic factors in colorectal cancer
- Herpesvirus Infections and Treatments
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- Cancer-related Molecular Pathways
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- Viral Infectious Diseases and Gene Expression in Insects
- Computational Drug Discovery Methods
- Kruppel-like factors research
- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Colorectal Cancer Treatments and Studies
- Thyroid Cancer Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Acute Myeloid Leukemia Research
- PI3K/AKT/mTOR signaling in cancer
- CRISPR and Genetic Engineering
Pasteur Institute of Iran
2016-2025
Biotechnology Research Center
2016-2025
Tehran University of Medical Sciences
2017-2018
GTx (United States)
2017
Shariati Hospital
2010
University of Tehran
2010
National Institute of Genetic Engineering and Biotechnology
2004
Colorectal cancer (CRC) is the third most frequently diagnosed malignancy worldwide. Currently, irinotecan (CPT-11) used alone or in combination with other drugs to treat patients advanced CRC. However, 5-year survival rate for metastatic CRC remains below 10 %, largely due chemotherapy resistance. Several genes, including ABCG2, CYP3A4, MCL1, and MLH1 contribute This study aimed identify microRNAs that simultaneously regulate expression of these genes irinotecan-resistant cell lines their...
Overexpression of DNA methyltransferase 1 (DNMT-1) is observed mostly in pancreatic cancer and it can cause tumor suppressor genes silencing this disease. Recent studies suggest that abnormal expressions microRNAs (miRs) are involved pathogenesis different types human cancers including cancer. In study we aimed to investigate the effect miR-148b -152 on reverting tumorigenic phenotype cell lines. order whether regulation DNMT-1, luciferase reporter assay was used confirmed DNMT-1 mRNA could...
Low-density lipoprotein receptor-related protein 6 (LRP6) is an important therapeutic target for diseases such as osteoporosis, Alzheimer, cancer, and neurodegenerative disease. Computational methods ligand-based structure-based virtual screening have been introduced extremely efficient accurate tool finding new drug targets candidates. In this study, we aimed to screen the National Cancer Institute (NCI) Diversity Set II parts of ZINC database by identify potential safe compounds that can...
It is observed that upregulation of DNMT3B enzyme in some cancers, including colon cancer, could lead to silencing tumor suppressor genes. MiR-339 and miR-766 have been predicted target 3′UTR gene. Luciferase reporter assay validated individual co-transfection miR-339 into the HEK293T cell reduced luciferase activity 26% ± 0.41%, 43% 0.42 64% 0.52%, respectively, compared control (P < 0.05). Furthermore, transduction expressing viruses cancer lines (SW480 HCT116) decreased expression (1.5,...
Oxaliplatin as a component of (Neo-) adjuvant chemotherapeutic regimens is administered to colorectal cancer patients. Unfortunately, the acquisition resistance this drug in nearly 90% metastatic patients rendered it an ineffective drug. Therefore, mechanisms should be elucidated. There are different genes like GSTP1 and ABCB1 which responsible for oxaliplatin resistance. We hypothesized that miR-129-5p, miR-302c-5p, miR-3664-5p, mir-3714 miR-513a-3p targeting gene, while was predicted...
Fc-fusion peptides, also known as peptibodies, are a promising new category of targeted therapeutics that offer alternatives to monoclonal antibodies (mAbs) for cancer treatment. This study focuses on an peptide consisting the Fc region IgG1 and epidermal growth factor receptor (EGFR)-targeting peptide, GE11, which was identified using phage display method demonstrated high affinity receptor. The fusion (FcIgG-GE11) successfully expressed in Escherichia coli purified ion-exchange...
Over the latest decade, role of microRNAs (miRNAs/miRs) has received more attention. miRNAs are small non-coding RNAs that may serve a as oncogenes or tumor suppressor genes. Certain regulate apoptosis pathway by influencing pro- anti-apoptotic We hypothesized increases in expression B cell lymphoma 2 (BCL2) and BCL2-like 1 (BCL2L1) genes, which have been reported various types cancer tissues, be due to downregulation certain miRNAs. The present study aimed identify target BCL2 BCL2L1 genes...
Abstract Circulating cell‐free DNA (cfDNA) has been considered as a diagnostic source to track genetic and epigenetic alterations in cancer. We aimed study mutation addition the methylation status promoter regions of RASSF1 SLC5A8 genes tissues circulating free samples patients affected with papillary thyroid carcinoma (PTC) nodules controls. BRAF V600E was studied by ARMS‐scorpion real‐time polymerase chain reaction method 57 PTC 45 nodule cases. Methylation analyzed methylation‐specific...
Successful delivery of irf5-siRNA by PEI-LBL-NCs resulted macrophages phenotype change from the M1 to M2 and accelerated wound healing.
Aim: The aim was to investigate the effect of miR-377 on DNMT1 expression and cancer phenotype in pancreatic cells. Materials & methods: Real-time PCR, luciferase assay, MTT Annexin-PI staining were used. Results: Decreased increased (verified as a target for mir-377) levels tissues cell lines comparison with normal confirmed be influenced by promoter methylation. Also hypermethylation BNIP3, SPARC, TFPI2 PENK promoters observed tumor samples but not which negatively correlated their...
Tamoxifen is a standard anti‐hormone treatment in estrogen receptor positive breast carcinoma patients. Unfortunately, about 50% of patients relapse during treatment. Promoter hypermethylation contributes to the epigenetic modulation tamoxifen resistance‐related genes. To evaluate contribution DNMTs expression and their promoter methylation as diagnostic biomarkers development malignancy resistance, present study was designed 107 tumors normal tissues were recruited. Methylation‐specific...
Colorectal cancer (CRC) is one of the most common malignancies, and third leading cause mortality worldwide. Timely detection CRC in patients with earlier stages provides highest rate survival. Epigenetic alterations are important occurrence progression CRC, represent primary modifications cells. Therefore, these cases thought to hold great promise as diagnostic biomarkers. It has been shown that SEPT9 NTRK3 genes aberrantly methylated their can be used biomarkers for early diagnosis CRC....
Colorectal cancer (CRC) is the third most prevalent all around world. Chemotherapy plays an essential role in treatment of CRC while Oxaliplatin, Irinotecan, and 5 – fluorouracil (5-FU) are commonly used chemotherapeutic drugs. However, chemo-resistance a major obstacle to successful therapy. It has been shown that inhibition Wnt signaling pathway can sensitize cells chemotherapy. Lymphoid enhancer factor (LEF1) member TCF/LEF transcription family mediating nuclear responses. The long...
BACKGROUND: Colorectal cancer (CRC) is known as one of the most leading causes death from in world. Wnt signaling pathway important activated this type cancer. The low-density lipoprotein (LDL) receptor-related protein-6 (LRP6) functions a co-rec eptor order to trigger pathway. OBJECTIVE: expression LRP6 was studied cell lines, tumoral and normal adjacent tissues patients affected with colorectal MATERIALS AND METHODS: assayed by quantitative Real time PCR. RESULTS: overexpressed at...
An increasing attitude towards oncolytic viruses (OVs) is witnessed following T-VEC's approval. In this study, we aimed to delete ICP47 and insert IL-12 in the ICP34.5 deleted HSV-1 backbone improve properties provide an immune-stimulatory effect respectively. The wild-type recombinant infected both cancerous, SW480 HCT116, non-cancerous, HUVEC, cell lines. Green-red Δ47/Δ34.5 was constructed by replacing with GFP. Both copies were replaced hIL12. Cytotoxicity growth kinetics of...
Introduction: Some gene expression regulation in cancers can be controlled by epigenetic change like methylation. PTEN promoter methylation and were evaluated endometrial cancer. Methods: The study was run on 39 tumor tissues of cancer patients 41 normal tissues. After total RNA extraction, cDNA synthesis done reverse transcription the (real-time PCR) using SYBER Green master mix. DNA extraction bisulfite treatment conducted semiquantified methylation-sensitive high-resolution melting...
Background: Myc (c-Myc) alone activates the embryonic stem cell-like transcriptional module in both normal and transformed cells. Its dysregulation might lead to increased cancer cells (CSCs) population some tumor Objective: In order investigate potential of decoy oligodeoxynucleotides for differentiation therapy, mouse (mESCs) were used this study as a model CSCs. To our best knowledge is first report outlining application transcription factor “TFD” strategy inducing mESCs. Methods: A...