A5027712512- Mesenchymal stem cell research
- Single-cell and spatial transcriptomics
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Molecular Biology Techniques and Applications
- Extracellular vesicles in disease
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
University of Southampton
2020-2023
Human bone marrow (BM)-derived stromal cells contain a population of skeletal stem (SSCs), with the capacity to differentiate along osteogenic, adipogenic, and chondrogenic lineages, enabling their application clinical therapies. However, current methods isolate enrich SSCs from human tissues remain, at best, challenging in absence specific SSC marker. Unfortunately, none proposed markers alone can homogeneous cell ability form bone, cartilage, adipose tissue humans. Here, we have designed...
There is a wealth of data indicating human bone marrow contains skeletal stem cells (SSC) with the capacity for osteogenic, chondrogenic and adipogenic differentiation. However, current methods to isolate SSCs are restricted by lack defined marker, limiting understanding SSC fate, immunophenotype, function clinical application. The study applied single-cell RNA-sequencing profile adult populations from 11 donors identified novel targets enrichment. Spherical nucleic acids were used detect...
Abstract There is a wealth of data indicating human bone marrow derived stromal cells (HBMSCs) contain the skeletal stem cell (SSC) with potential to differentiate along osteogenic, adipogenic and chondrogenic lineages. However, despite these advances, current methods isolate (SSCs) from tissues have proved challenging as no single specific marker has been identified limiting understanding SSC fate, immunophenotype widespread clinical application cells. While number surface markers can...