A5022316074- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- Microfluidic and Capillary Electrophoresis Applications
- Diet and metabolism studies
- Gut microbiota and health
- Advanced Drug Delivery Systems
- Proteins in Food Systems
- Cellular Mechanics and Interactions
- Innovative Microfluidic and Catalytic Techniques Innovation
- Bone health and osteoporosis research
- Mesenchymal stem cell research
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- Planarian Biology and Electrostimulation
- Nanoparticles: synthesis and applications
- Proteoglycans and glycosaminoglycans research
- Analytical Chemistry and Sensors
- Biochemical Analysis and Sensing Techniques
- Dietary Effects on Health
- Food composition and properties
- Nerve injury and regeneration
- Intravenous Infusion Technology and Safety
- Neuroscience and Neural Engineering
International Iberian Nanotechnology Laboratory
2019-2024
University of Southampton
2016-2021
Southampton General Hospital
2016-2019
Centre For Human Genetics
2016-2019
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2018
Universidade do Porto
2018
Institute of Mechanical Engineering and Industrial Mangement
2015
Skeletal stem cells (SSCs) are present in bone marrow (BM) and offer great potential for regenerative therapies. However, the absence of a unique marker, current sorting approaches remain challenging quest simple strategies to deliver SSCs with consistent regeneration differentiation capacities. Microfluidics offers possibility sort marker-free, based on intrinsic biophysical properties. Recent studies indicate that stiffer than leukocytes contained within larger cell fraction BM. This paper...
Skeletal stem cells (SSCs) are a sub-population of mesenchymal stromal (MSCs) present in bone marrow with multipotent differentiation potential. A current unmet challenge hampering their clinical translation remains the isolation homogeneous populations SSCs, vitro, consistent regeneration and capacities. Cell stiffness has been shown to play an important role cell separation using microfluidic techniques such as inertial focusing or deterministic lateral displacement. Here we report that...
The use of nanomaterials in the food and feed chain is expected to keep increasing years come. While this brings exciting prospects both industry consumers, it also entails important considerations regarding their risk assessment potential detrimental effects following human exposure. Aiming standardise nanomaterials, European Food Safety Authority (EFSA) has recently published a new guidance where vitro tests are indicated play key role. Here, we summarise latest EFSA aiming bring wider...
Breast cancer remains a leading cause of cancer-related mortality, with disease progression and metastasis posing significant challenges for treatment. Three-dimensional (3D) models have emerged as valuable tools studying cell biology in physiologically relevant microenvironment. Studying the tumour heterogeneity metabolic adaptations at single-cell level can be crucial to identify factors driving metastatic progression. Here, we present novel approach generate single cell-derived breast...
We present a novel microfluidic platform combining Digestion-Chip and Gut-Chip. The setup is able to simulate digestion test digested samples in the cell-based Gut-Chip evaluate intestinal permeability without compromising cell viability.
Abstract Reliable in-vitro digestion models that are able to successfully replicate the conditions found in human gastrointestinal tract key assess fate and efficiency of new formulations aimed for oral consumption. However, current either lack capability crucial dynamics or require large volumes sample/reagents, which can be scarce when working with nanomaterials under development. Here, we propose a miniaturised system, digestion-chip, based on incubation chambers integrated...
The capacity of bone and cartilage to regenerate can be attributed skeletal stem cells (SSCs) that reside within the marrow (BM). Given SSCs are rare lack specific surface markers, antibody-based sorting has failed deliver cell purity required for clinical translation. Microfluidics offers new methods isolating based on biophysical features including, but not limited to, size, electrical properties stiffness. Here we report characterization dielectric unexpanded using single-cell...
Oral administration remains the most common mode of drug delivery. However, orally administered bioactive compounds must first survive digestion and then be absorbed at intestine in order to reach other tissues or organs. The efficiency both processes can improved by encapsulation conjugation with polymeric nanoparticles. Here we report synthesis amphiphilic hyaluronic acid (HyA) nanogels as nanocarriers for delivery.HyA were prepared self-assembly from HyA conjugates produced grafting...
Polydimethylsiloxane (PDMS) is ubiquitously used in microfluidics. However, PDMS porous and hydrophobic, potentially leading to small molecule partitioning. Although many studies addressed this issue suggested surface/bulk modifications overcome it, most were not quantitative, did address which variables besides hydrophobicity governed absorption, no modification has been shown completely obviate it. We evaluated qualitatively (confocal microscopy) quantitatively (fluorescence spectroscopy)...
Human bone marrow (BM)-derived stromal cells contain a population of skeletal stem (SSCs), with the capacity to differentiate along osteogenic, adipogenic, and chondrogenic lineages, enabling their application clinical therapies. However, current methods isolate enrich SSCs from human tissues remain, at best, challenging in absence specific SSC marker. Unfortunately, none proposed markers alone can homogeneous cell ability form bone, cartilage, adipose tissue humans. Here, we have designed...
Short chain fatty acids (SCFA) are hypothesised to play a key role in the microbiota-gut-brain crosstalk. However, studies that evaluate effect of microbiota-targeted interventions, such as prebiotics, probiotics or diet still sparse, particularly humans. In this work, products fermentation prebiotic-enriched media by bacteria from primary human faecal samples were tested on differentiated Caco-2/HT29-MTX co-cultures. The prebiotics raftilose and fructo-oligosaccharides (FOS) tested,...
This study aimed to evaluate the disparity of expectations and basic knowledge prenatal ultrasound (US) screening among pregnant women make a comparison with current scientific national recommendations. We hypothesize that sociodemographic factors, including age, education, professional occupation, may be associated different levels knowledge.
Reliable in-vitro digestion models that are able to successfully replicate the conditions found in human gastrointestinal tract (GIT) key assess fate and efficiency of new formulations aimed for oral consumption. However, current either lack capability crucial dynamics or require large volumes sample/reagents, which can be scarce when working with nanomaterials under development. Here, we propose a miniaturised system, digestion-chip, based on incubation chambers integrated...
Abstract Neurological disorders, a leading global cause of death, encompass conditions affecting the peripheral and central nervous systems (PNS CNS, respectively). Limited axon regeneration is significant challenge in these it linked to proteins like PTEN. RNA-based therapeutics, particularly siRNAs, hold potential for silencing inhibitory pathways, but their clinical application hindered by poor stability cellular uptake. Our study addressed this with development novel, fully biodegradable...
Gene therapy using small interfering RNA (siRNA) holds promise for treating neurological disorders by silencing specific genes, such as the phosphatase and tensin homolog ( PTEN ) gene, which restricts axonal growth. Yet, delivering siRNA to neurons efficiently is challenging due premature degradation unspecific delivery. Chitosan-based delivery systems have shown great potential their well-established biocompatibility. However, limited transfection efficiency lack of neuronal tropism...
Motivated by the need for improved platforms in biomedical research, this study addresses challenges associated with traditional Ussing chamber systems, widely used studying biological barriers like epithelial barrier of gut. These include complexity, high sample volumes, and limited compatibility. By combining stereolithography soft lithography techniques, a microfluidic is manufactured, overcoming these limitations, incorporating compatibility microscopy. Validation through...
ABSTRACT Human bone marrow (BM) derived stromal cells contain a population of skeletal stem (SSCs), with the capacity to differentiate along osteogenic, adipogenic and chondrogenic lineages enabling their application clinical therapies. However, current methods, isolate enrich SSCs from human tissues remain, at best, challenging in absence specific SSC marker. Unfortunately, none proposed markers, alone, can homogenous cell ability form bone, cartilage, adipose tissue humans. Here, we have...
The central nervous system (CNS) and the periphery are closely associated in regulation of body homeostasis. In fact, both physiological pathological conditions, CNS showed to be targeted by peripheral molecules, which able generate adaptive responses under tight control hypothalamus. spite having a well-recognized importance, mechanisms underlying this communication still unclear. Two major routes have been proposed explain transmission information from CNS: sensory pathway, through nerves...