A5009488449- Osteoarthritis Treatment and Mechanisms
- Inflammatory mediators and NSAID effects
- Cytokine Signaling Pathways and Interactions
- Fatty Acid Research and Health
- Cancer-related molecular mechanisms research
- Peroxisome Proliferator-Activated Receptors
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Eicosanoids and Hypertension Pharmacology
- Bone Metabolism and Diseases
- 3D Printing in Biomedical Research
- RNA Interference and Gene Delivery
- Rheumatoid Arthritis Research and Therapies
- Mesenchymal stem cell research
- Cellular Mechanics and Interactions
- RNA modifications and cancer
- Nitric Oxide and Endothelin Effects
- Natural product bioactivities and synthesis
- Biochemical effects in animals
- Calcium Carbonate Crystallization and Inhibition
- Protein Degradation and Inhibitors
- Histone Deacetylase Inhibitors Research
- Hypothalamic control of reproductive hormones
- Adolescent and Pediatric Healthcare
Instituto de Investigación Biomédica de A Coruña
2009-2024
Complexo Hospitalario Universitario A Coruña
2013-2024
Universidade da Coruña
2002-2024
University of Southampton
2010-2020
Southampton General Hospital
2017
Instituto de Investigación Sanitaria de Santiago
2011-2015
Complejo Hospitalario Universitario de Santiago
2011
Hospital San Juan de la Cruz
2003
Osteoarthritis (OA) is a degenerative rheumatic disease that associated with extracellular matrix degradation and chondrocyte apoptosis in the articular cartilage. The role of mitochondria diseases widely recognized. We undertook this study to evaluate mitochondrial function normal OA chondrocytes examine age-related changes mitochondria.Mitochondrial was evaluated by analyzing respiratory chain enzyme complexes citrate synthase (CS) activities as well membrane potential (Delta Psi m)....
The role of DNA methylation in the regulation catabolic genes such as MMP13 and IL1B, which have sparse CpG islands, is poorly understood context musculoskeletal diseases. We report that demethylation specific sites at −110 bp −299 proximal IL1B promoters, respectively, detected by situ analysis chondrocytes obtained directly from human cartilage, strongly correlated with higher levels gene expression. status these had a significant impact on promoter activities chondrocytes, revealed...
To investigate whether the abnormal expression of inducible nitric oxide synthase (iNOS) by osteoarthritic (OA) human chondrocytes is associated with changes in DNA methylation status promoter and/or enhancer elements iNOS.
Objective To investigate whether the changes in collagen gene expression osteoarthritic (OA) human chondrocytes are associated with DNA methylation status COL2A1 enhancer and COL9A1 promoter. Methods Expression levels were determined using quantitative reverse transcription–polymerase chain reaction, percentage of was quantified by pyrosequencing. The effect CpG on promoter activity a CpG‐free vector; cotransfections vectors encoding SOX9, hypoxia‐inducible factor 1α (HIF‐1α), HIF‐2α carried...
The Runt-related transcription factor 2 (RUNX2) is critical for bone formation as well chondrocyte maturation. Matrix metalloproteinase (MMP)-13 a major contributor to cartilage degradation in osteoarthritis (OA). We and others have shown that the abnormal MMP13 gene expression OA chondrocytes controlled by changes DNA methylation status of specific CpG sites proximal promoter, actions different transactivators, including RUNX2. present study aimed determine influence RUNX2 promoter on...
In culture isolated bone marrow mesenchymal stem cells (more precisely termed skeletal cells, SSCs) spontaneously differentiate into fibroblasts, preventing the growth of large numbers multipotent SSCs for use in regenerative medicine. However, mechanisms that regulate expansion SSCs, while maintaining multipotency and fibroblastic differentiation are poorly understood. Major hurdles to understanding how maintenance is regulated (a) from heterogeneous populations with different proliferative...
Articular cartilage injury can result in chondrocyte loss and diminishment of specialised extracellular matrix, which progress to an osteoarthritic (OA) phenotype. Stem cells have emerged as a favourable approach for articular regeneration. Identification miRNAs influence stem cell fate offers new approaches application regenerate cartilage. Skeletal (SSCs) isolated from human bone marrow were cultured high density micromass' using TGF-β3 induce chondrogenesis. qPCR TaqMan used assess...
Many skeletal tissue regenerative strategies centre around the multifunctional properties of bone marrow derived stromal cells (BMSC) or mesenchymal stem/stromal (MSC)/bone stem (SSC). Specific identification these particular has been inconclusive. However, enriching heterogeneous cell populations with characterised progenitor markers a contributing factor in successful regeneration and repair strategies. In current studies we have isolated, enriched ovine (oBMSCs) using specific antibody,...
Abstract Introduction The pathogenesis of osteoarthritis (OA) is characterized by the production high amounts nitric oxide (NO), as a consequence up-regulation chondrocyte-inducible synthase ( iNOS ) induced inflammatory cytokines. NO donors represent powerful tool for studying role in cartilage vitro . There no consensus about effects on articular part because differences between available. aim this work to compare metabolic profile traditional and new generation see which one points out...
The capacity of bone and cartilage to regenerate can be attributed skeletal stem cells (SSCs) that reside within the marrow (BM). Given SSCs are rare lack specific surface markers, antibody-based sorting has failed deliver cell purity required for clinical translation. Microfluidics offers new methods isolating based on biophysical features including, but not limited to, size, electrical properties stiffness. Here we report characterization dielectric unexpanded using single-cell...
The regenerative potential of skeletal stem cells provides an attractive prospect to generate bone tissue needed for musculoskeletal reparation. A central issue remains efficacious, controlled cell differentiation strategies aid progression therapies the clinic. nacre surface from Pinctada maxima shells is known enhance formation. However, date, there a paucity information on role topography P. surfaces, and prism. To investigate this, prism topographical features were replicated onto...
Epigenetic modifications are heritable changes in gene expression without DNA sequence. methylation has been implicated the control of several cellular processes including differentiation, regulation, development, genomic imprinting and X-chromosome inactivation. Methylated cytosine residues at CpG dinucleotides commonly associated with repression; conversely, strategic loss during development could lead to activation lineage-specific genes. Evidence is emerging that bone growth programmed;...
Spatiotemporal control of drug delivery is important for a number medical applications and may be achieved using polymersome nanoparticles (PMs). Wnt signalling molecular pathway activated in various physiological processes, including bone repair, that requires precise activation. Here, we hypothesise PMs can stably loaded with small molecule agonist, 6-bromoindirubin-3'-oxime (BIO), activate promoting the osteogenic differentiation human primary marrow stromal cells (BMSCs). We showed...
Osteoarthritis (OA) is a chronic disease that affects articular cartilage, causing its degeneration. Although OA one of the most prevalent pathologies globally, there are no definitive treatments available. Recently, research has focused on elucidating complex interplay takes place between inflammatory processes and epigenetic regulation, showing histone post-translational modifications (PTMs) can exert pronounced effect expression OA-related genes. chondrocytes enhance production...
Recent studies have shown that dietary patterns confer protection from certain chronic diseases related to oxidative stress, the immune system and low-grade inflammatory diseases. The aim of this study was evaluate anti-inflammatory potential capacity attenuate cartilage degradation using extra-virgin olive oil-derived polyphenols for treatment osteoarthritis. Results show both nutraceuticals ligstroside aglycone acetylated showed an profile. Acetylated significantly reduced expression...