A5002684517- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Gene Regulatory Network Analysis
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Cell Image Analysis Techniques
- Mesenchymal stem cell research
- Nanoparticle-Based Drug Delivery
- 3D Printing in Biomedical Research
- CRISPR and Genetic Engineering
- Microfluidic and Bio-sensing Technologies
- Hematopoietic Stem Cell Transplantation
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Immune responses and vaccinations
- Magnetic Properties and Synthesis of Ferrites
- Atomic and Molecular Physics
- Iron oxide chemistry and applications
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Speech and dialogue systems
- Molecular Biology Techniques and Applications
- Telomeres, Telomerase, and Senescence
- Advanced Fluorescence Microscopy Techniques
Budapest University of Technology and Economics
2024
University of Southampton
2012-2023
RWTH Aachen University
2019-2023
Universitätsklinikum Aachen
2020
Freie Universität Berlin
2012-2015
Southampton General Hospital
2014
Fraunhofer Society
2012
University of Würzburg
2009-2010
Fraunhofer Institute for Biomedical Engineering
2009
Johannes Gutenberg University Mainz
1992
The recent success of channelrhodopsin in optogenetics has also caused increasing interest enzymes that are directly activated by light. We have identified the genome bacterium Beggiatoa a DNA sequence encoding an adenylyl cyclase linked to BLUF (blue light receptor using FAD) type sensor domain. In Escherichia coli and Xenopus oocytes, this photoactivated (bPAC) showed activity is low darkness but increased 300-fold This enzymatic decays thermally within 20 s parallel with red-shifted...
During acute myelosuppression or thrombocytopenia, bone marrow (BM) hematopoietic cells respond rapidly to replenish peripheral blood platelets. While the cytokine thrombopoietin (Thpo) both regulates platelet production and maintains HSC potential, whether Thpo controls megakaryocyte (Mk)-lineage differentiation of HSCs is unclear. Here, we show that upregulates mitochondrial activity in HSCs, an accompanied by Mk lineage. Moreover, unperturbed hematopoiesis, with high exhibit Mk-lineage...
MicroRNAs (miRs) play a pivotal role in variety of biological processes including stem cell differentiation and function. Human foetal femur derived skeletal cells (SSCs) display enhanced proliferation multipotential capacity indicating excellent potential as candidates for tissue engineering applications. This study has examined the expression miRs human SSC along chondrogenic osteogenic lineages. Cells isolated from epiphyseal region expressed higher levels genes associated with...
Abstract Polymersome nanoparticles (PMs) are attractive candidates for spatio-temporal controlled delivery of therapeutic agents. Although many studies have addressed cellular uptake solid nanoparticles, there is very little data available on intracellular release molecules encapsulated in membranous carriers, such as polymersomes. Here, we this by developing a quantitative assay based the hydrophilic dye, fluorescein. Fluorescein was stably PMs mean diameter 85 nm, with minimal leakage...
Mutations in HPRT1, a gene encoding rate-limiting enzyme for purine salvage, cause Lesch-Nyhan disease which is characterized by self-injury and motor impairments. We leveraged stem cell genetic engineering technologies to model the isogenic patient-derived forebrain midbrain types. Dopaminergic progenitor cells deficient HPRT showed decreased intensity of all developmental cell-fate markers measured. Metabolic analyses revealed significant loss derivatives, except hypoxanthine, impaired...
Abstract Langerhans cells (LC) can prime tolerogenic as well immunogenic responses in skin, but the genomic states and transcription factors (TF) regulating these context-specific are unclear. Bulk single-cell transcriptional profiling demonstrates that human migratory LCs robustly programmed for MHC-I MHC-II antigen presentation. Chromatin analysis reveals enrichment of ETS-IRF AP1-IRF composite regulatory elements antigen-presentation genes, coinciding with expression TFs, PU.1, IRF4 BATF3...
Abstract Biomedical research often involves conducting experiments on model organisms in the anticipation that biology learnt will transfer to humans. Previous comparative studies of mouse and human tissues were limited by use bulk-cell material. Here we show learning—the branch machine learning concerns passing information from one domain another—can be used efficiently map bone marrow between species, using data obtained single-cell RNA sequencing. We first trained a multiclass logistic...
Monodisperse small iron oxide nanoparticles functionalized with dendritic polyglycerol (dPG) or sulfate (dPGS) are prepared. They highly stable in aqueous solutions as well physiological media. In particular, oleic acid capped particles (core diameter = 11 ± 1 nm) were modified by a ligand exchange process one pot synthesis dPG and dPGS bearing phosphonate anchor groups. Dynamic light scattering measurements performed water different biological media demonstrate that the hydrodynamic of is...
A number of important pluripotency regulators, including the transcription factor Nanog, are observed to fluctuate stochastically in individual embryonic stem cells. By transiently priming cells for commitment different lineages, these fluctuations thought be maintenance of, and exit from, pluripotency. However, because temporal changes intracellular protein abundances cannot measured directly live cells, typically assessed using genetically engineered reporter cell lines that produce a...
The molecular regulatory network underlying stem cell pluripotency has been intensively studied, and we now have a reliable ensemble model for the 'average' pluripotent cell. However, evidence of significant cell-to-cell variability suggests that activity this varies within individual cells, leading to differential processing environmental signals in fates. Here, adapt method originally designed face recognition infer patterns cells from single-cell expression data. Using identify three...
Langerhans cells (LCs) reside in the epidermis as a dense network of immune system sentinels, coordinating both immunogenic and tolerogenic responses. To determine molecular switches directing induction LC activation, we performed mathematical modelling gene regulatory networks identified by single cell RNA sequencing LCs exposed to TNF-alpha, key pro-inflammatory signal produced skin. Our approach delineated three programmes phenotypic activation (immunogenic, or ambivalent), confirmed that...
Iron oxide nanoparticles (FeOx-NP) are applied in medicine as contrast agents magnetic resonance imaging (MRI) where they reduce the spin–spin relaxation time (T2-time) of absorbing tissue. Hence, control their properties is essential for these applications. Magnetic strongly depend on particle size and shape well surface functionalization iron nanoparticles. Especially, structural disorder region close to (1–2 nm) lead usually a reduced magnetization compared corresponding bulk material....
Pirouette coupling involves Dean flow for cell and reporter bead inertial ordering efficient co-encapsulation, achieving a throughput of 1 million cells per hour, 2.5% multiplet rate 70% capture efficiency.
There is a wealth of data indicating human bone marrow contains skeletal stem cells (SSC) with the capacity for osteogenic, chondrogenic and adipogenic differentiation. However, current methods to isolate SSCs are restricted by lack defined marker, limiting understanding SSC fate, immunophenotype, function clinical application. The study applied single-cell RNA-sequencing profile adult populations from 11 donors identified novel targets enrichment. Spherical nucleic acids were used detect...
Pluripotent stem cells (PSCs) are a popular model system for investigating development, tissue regeneration, and repair. Although much is known about the molecular mechanisms that regulate balance between self-renewal lineage commitment in PSCs, spatiotemporal integration of responsive signaling pathways with core transcriptional regulatory networks complex only partially understood. Moreover, measurements made on populations reveal average properties underlying networks, obscuring their...
Abstract Pluripotent stem cells are able to self-renew indefinitely in culture and differentiate into all somatic cell types vivo . While much is known about the molecular basis of pluripotency, mechanisms lineage commitment complex only partially understood. Here, using a combination single profiling mathematical modeling, we examine differentiation dynamics individual mouse embryonic (ESCs) as they progress from ground state pluripotency along neuronal lineage. In accordance with previous...
Modern single cell experiments have revealed unexpected heterogeneity in apparently functionally 'pure' populations. However, we are still lacking a conceptual framework to understand this heterogeneity. Here, propose that cellular memories-changes the molecular status of response stimulus, modify ability respond future stimuli-are an essential ingredient any such theory. We illustrate idea by considering simple age-structured model stem proliferation takes account mitotic memories. Using...
Abstract Kabuki syndrome is frequently caused by loss-of-function mutations in one allele of histone 3 lysine 4 (H3K4) methyltransferase KMT2D and associated with problems neurological, immunological skeletal system development. We generated heterozygous knockout patient-derived cell models to investigate the role reduced dosage stem cells. discovered chromosomal locus-specific alterations gene expression, specifically a 110 Kb region containing Synaptotagmin (SYT3), C-Type Lectin Domain...