A5074310224- Growth Hormone and Insulin-like Growth Factors
- Bariatric Surgery and Outcomes
- Diet and metabolism studies
- Diabetes Treatment and Management
- Sexual Differentiation and Disorders
- Diabetes Management and Research
- Bone health and osteoporosis research
- Estrogen and related hormone effects
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Tuberous Sclerosis Complex Research
- Bone health and treatments
- Diabetes and associated disorders
- Metabolism, Diabetes, and Cancer
- Cancer, Hypoxia, and Metabolism
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Regulation of Appetite and Obesity
- Metabolism and Genetic Disorders
- Blood groups and transfusion
- Thyroid Cancer Diagnosis and Treatment
- Urological Disorders and Treatments
- T-cell and B-cell Immunology
- Lipid metabolism and disorders
- Biochemical and Molecular Research
- Birth, Development, and Health
University of Cincinnati Medical Center
2016-2025
Lund University
2024
University of Cincinnati
2013-2023
University of Arizona
2021
Cincinnati VA Medical Center
2015
Diabetes Australia
2008
United States Department of the Navy
2005-2006
Naval Medical Center San Diego
2005-2006
Cincinnati Children's Hospital Medical Center
1991-2004
Weatherford College
1998
Mutations in the estrogen-receptor gene have been thought to be lethal. A 28-year-old man whose estrogen resistance was caused by a disruptive mutation underwent studies of pituitary-gonadal function and bone density received transdermal for six months. Estrogen-receptor DNA, extracted from lymphocytes, evaluated analysis single-strand-conformation polymorphisms direct sequencing.
Abstract The BRAFT1799A mutation is the most common genetic alteration in papillary thyroid carcinomas (PTC). It also found a subset of microcarcinomas, consistent with role tumor initiation. PTCs are often invasive and present at more advanced stage. high prevalence tall-cell variant poorly differentiated undifferentiated arising from PTCs. To explore BRAFV600E cancer pathogenesis, we targeted its expression to cells transgenic FVB/N mice bovine thyroglobulin promoter. Two Tg-BRAFV600E...
Background: A hallmark of cancer is the presence an immunosuppressive tumor microenvironment (TME). Immunosuppressive M2 macrophages (MΦs) in TME facilitate escape from immune surveillance and promote growth; therefore, TME-induced immunosuppression a potent immunotherapeutic approach to treating cancer. Methods: Cancer cell-secreted proteins were detected by using liquid chromatography–mass spectrometry (LC-MS). Neutralizing antibodies (nAbs) used assess which involved MΦs polarization...
Abstract Heat-stable, chemotactically active peptides have been obtained from Escherichia coli culture filtrates. They range in size between 150 and 1500 daltons are anionic at neutral pH. Free carboxyl groups but not free amino appear to be required for activity. The N-terminal group may blocked. There do internal aromatic or basic residues the fractions. A highly purified, completely characterized, fraction was found contain aspartic acid, serine, glutamic alanine, glycine.
Background While estrogens protect against coronary artery disease in women, it is unclear whether they influence cardiovascular function men. The present report describes vascular abnormalities and the lipoprotein profile of a male patient with estrogen insensitivity caused by disruptive mutation estrogen-receptor gene. Methods Results Stress thallium scintigraphy, echocardiography, electron-beam computed tomography (CT) scanning arteries detailed analysis were performed. Electron-beam CT...
Roux-en-Y gastric bypass (RYGB) and vertical sleeve gastrectomy (VSG) are effective weight loss surgeries that also improve glucose metabolism. Rapid, early rises of circulating insulin glucagon-like peptide-1 (GLP-1) concentrations following food ingestion characteristic these procedures. The purpose the current study was to test hypothesis postprandial hormone release is due increased nutrient emptying from stomach. Radioscintigraphy chemical radiolabeled tracers were used examine in rat...
Smith, Eric P.; Boyd, Jeff; Frank, Graeme R.; Takahashi, Hiroyuki; Cohen, Robert M.; Specker, Bonny; Williams, Timothy C.; Lubahn, Dennis B.; Korach, Kenneth S.Author Information
Papillary thyroid carcinomas are associated with nonoverlapping activating mutations of RET, NTRK, RAS and BRAF, which altogether present in approximately 70% cases. We postulated that compounds inhibit a distal effector the mitogen-activated protein kinase (MAPK) pathway would growth tumorigenicity human cancer cell lines RET or BRAF.We first examined effects AAL-881 LBT-613, two inhibitors RAF activity, on RAF-MAPK/extracellular signal-regulated (ERK) (MEK)-ERK activation PCCL3 cells after...
OBJECTIVE—To determine whether interindividual heterogeneity in the erythrocyte (red blood cell [RBC]) transmembrane glucose gradient might explain discordances between A1C and glycemic control based on measured fructosamine. RESEARCH DESIGN AND METHODS—We modeled relationship plasma RBC as concentration distribution (Ci-to-Co ratio) of a nonmetabolizable analog 14C-3-O-methyl (14C-3OMG) inside (Ci) outside (Co) RBCs vitro. We examined that degree glycation hemoglobin comparison with serum...
A peptide that is recognized by antibodies to human somatomedin-C/insulin-like growth factor I (Sm-C/IGF-I) has been partially purified from cultured Sertoli cells prepared sexually immature rats. The mol wt of this about 25,000, as determined gel filtration chromatography and immunoblot analysis samples resolved polyacrylamide electrophoresis. Isoelectric focusing indicated the isoelectric point was near neutrality. However, a smaller 8,000 cross-reacted with Sm-C/IGF-I, released after in...
Pharmacological and surgical interventions that increase glucagon-like peptide 1 (GLP-1) action are effective to improve glucose homeostasis in type 2 diabetes mellitus. In light of this, nutritional strategies enhance postprandial GLP-1 secretion, particularly the context diet-induced obesity, may provide an alternative therapeutic approach. Importantly, recent evidence suggests amino acid l-arginine, a well-known insulin secretagogue, can also stimulate release from isolated rat intestine....
Morbid obesity is a barrier to kidney transplant in patients with end-stage renal disease (ESRD). Laparoscopic sleeve gastrectomy (SG) an increasingly considered intervention, but the safety and long-term outcomes are uncertain. We reviewed prospectively collected data on ESRD chronic (CKD) undergoing SG from 2011 2018. There were 198 45 CKD (stages 1-4) who met National Institutes of Health guidelines for bariatric surgery underwent SG; 72% 48% achieved body mass index ≤ 40 35 kg/m2 ,...
A previous report from our laboratory described an approximately 30 kilodalton (kDa) insulin-like growth factor binding protein (IGFBP) that inhibited the of I (IGF-I) by its receptor and was secreted a subline B104 rat neuronal cell line. To better understand biology this IGFBP, it purified media conditioned these cells, chemical biological properties were examined. The IGFBP existed as 24 kDa form 28 when analyzed ligand blot. Deglycosylation studies indicated species N-linked glycosylated...
The kindred described is the only known instance of a germ line loss function mutation estrogen receptor (ER)-alpha.Our objective was to assess impact in ER-alpha gene on histomorphometry, bone volumetric density, geometry and skeletal growth, heterozygosity spine density adult height an extended pedigree.A longitudinal follow-up propositus with homozygous single contact evaluation were performed.Iliac crest biopsy peripheral quantitative computed tomography serial measures areal mineral...
The CD40 gene, an important immune regulatory is also expressed and functional on nonmyeloid-derived cells, many of which are targets for tissue-specific autoimmune diseases, including β cells in type 1 diabetes, intestinal epithelial Crohn's disease, thyroid follicular Graves' disease (GD). Whether target tissue expression plays a role etiology has yet to be determined. In this study, we show that overexpression key the autoimmunity. Using murine model GD, demonstrated thyroidal augmented...
Organismal stress initiates a tightly orchestrated set of responses involving complex physiological and neurocognitive systems. Here, we present evidence for glucagon-like peptide 1 (GLP-1)-mediated paraventricular hypothalamic circuit coordinating the global response. The GLP-1 receptor ( Glp1r ) in mice was knocked down neurons expressing single-minded 1, transcription factor abundantly expressed nucleus (PVN) hypothalamus. Mice with 1-mediated knockdown had reduced...
Morbid obesity is a barrier to kidney transplantation due inferior outcomes, including higher rates of new-onset diabetes after (NODAT), delayed graft function (DGF), and failure. Laparoscopic sleeve gastrectomy (LSG) increases transplant eligibility by reducing BMI in candidates, but the effect surgical weight loss on posttransplantation outcomes unknown. Reviewing single-center medical records, we identified all patients who underwent LSG before from 2011-2016 (n = 20). Post-LSG recipients...
Hemolysis is a key feature of sickle cell anemia (HbSS). Direct quantitation hemolysis could be used as an objective outcome in clinical trials new therapeutics for HbSS and would also enable better human studies the pathogenesis complications that are ostensibly hemolysis‐related, such pulmonary hypertension. However, contemporary have only surrogate markers rather than direct measurements RBC survival. We directly quantified by measuring survival age cohort RBCs labeled with stable...