A5063252467- Aquaculture disease management and microbiota
- Microbial infections and disease research
- Myxozoan Parasites in Aquatic Species
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Parasite Biology and Host Interactions
- Genomics and Phylogenetic Studies
- Animal Virus Infections Studies
- Ichthyology and Marine Biology
- Aquatic Invertebrate Ecology and Behavior
- Aquaculture Nutrition and Growth
- Geological and Geophysical Studies
- Paleontology and Evolutionary Biology
- Urinary Bladder and Prostate Research
- Urinary Tract Infections Management
- Sustainable Development and Environmental Policy
- Facial Rejuvenation and Surgery Techniques
- Viral Infections and Vectors
- Obesity and Health Practices
- Pelvic floor disorders treatments
- Congenital Heart Disease Studies
- Pulmonary Hypertension Research and Treatments
- Autoimmune Bullous Skin Diseases
- Vascular Anomalies and Treatments
Fisheries and Oceans Canada
2016-2024
Pacific Environment
2024
Erlanger Health System
2023
Cork University Hospital
2022
University of Illinois Chicago
2020
University of Idaho
2012-2015
Oregon Department of Fish and Wildlife
2014
Oregon State University
2014
Florida Fish and Wildlife Conservation Commission
2007-2008
University of South Florida St. Petersburg
2007
BackgroundThe International Liaison Committee on Resuscitation has called for a randomised trial of delivery to cardiac arrest centre. We aimed assess whether expedited centre compared with current standard care following resuscitated reduces deaths.MethodsARREST is prospective, parallel, multicentre, open-label, superiority trial. Patients (aged ≥18 years) return spontaneous circulation out-of-hospital without ST elevation were randomly assigned (1:1) at the scene their by London Ambulance...
Phage integrase genes often play a role in the establishment of lysogeny temperate phage by catalyzing integration into one host's replicons. To investigate gene expression, an induced viral metagenome from Tampa Bay was sequenced 454/Pyrosequencing. The sequencing yielded 294,068 reads with 6.6% identifiable. One hundred-three sequences had significant similarity to integrases BLASTX analysis (e < or =0.001). Four strongest amino-acid level were selected and real-time PCR primers probes...
The health risks of severe obesity can be reduced with metabolic and bariatric surgery, but it is uncertain which operation most effective or cost-effective. We aimed to compare Roux-en-Y gastric bypass, adjustable banding, sleeve gastrectomy in patients obesity. By-Band-Sleeve a pragmatic, multi-centre, open-label, randomised controlled trial conducted 12 hospitals the UK. Eligible participants were adults (aged ≥18 years) meeting national criteria for surgery. Initially, 2-group (Roux-en-Y...
Flavobacterium psychrophilum is the causative agent of bacterial coldwater disease and can cause significant mortality in salmonid aquaculture. To better evaluate prevention or treatment methods for F. laboratory, a waterborne challenge model that mimics natural outbreak needed. Here we report on development which incorporated variables may influence success: specifically, scarification prior to exposure culture under iron-limited conditions potentially increase probability establishing...
DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 122:213-221 (2017) - DOI: https://doi.org/10.3354/dao03076 Transmission potential infectious hematopoietic necrosis virus in APEX-IHN®-vaccinated Atlantic salmon Amy Long1,*, Jon Richard1, Laura Hawley1, Scott E. LaPatra2, Kyle A. Garver1 1Fisheries and Oceans Canada, Pacific Biological Station, Nanaimo, BC V9T 6N7,...
Abstract While co‐infections are common in both wild and cultured fish, knowledge of the interactive effects multiple pathogens on host physiology, gene expression immune response is limited. To evaluate impact co‐infection survival, physiology expression, sockeye salmon Oncorhynchus nerka smolts were infected with louse Lepeophtheirus salmonis (V−/SL+), infectious hematopoietic necrosis virus (IHNV; V+/SL−), (V+/SL+), or neither (V−/SL−). Survival V+/SL+ group was significantly lower than...
In recent years, Pacific oyster growers in British Columbia (BC), Canada have experienced devastating losses due to summer mortality syndrome. While anecdotal evidence suggests that intertidally-grown oysters may fare better during mass events than deep-water counterparts, there remains a lack of research examining how different culture conditions influence severity. To address this, we compared growth, condition, histopathology, reproductive status, and survival between intertidally-...
ABSTRACT The genome for the marine pseudotemperate member of Siphoviridae φHSIC has been sequenced using a combination linker amplification library construction, restriction digest and primer walking. enters into pseudolysogenic relationship with its host, Listonella pelagia , characterized by sigmoidal growth curves producing >10 9 cells/ml 11 phage/ml. (37,966 bp; G+C content, 44%) contained 47 putative open reading frames (ORFs), 17 which had significant BLASTP hits in GenBank,...
Abstract The etiological agent of bacterial coldwater disease (BCWD), Flavobacterium psychrophilum, can be transmitted both vertically and horizontally. Outbreaks BCWD result in significant losses salmonid aquaculture. Reduction outbreaks fry may possible through implementation a management strategy which progeny heavily infected broodstock are culled from the general population. Diagnostic assays to quantify F. psychrophilum concentrations tissue samples confirm presence bacterium ovarian...
Abstract The salmon louse Lepeophtheirus salmonis, a type of sea lice (family Caligidae), is enzootic in marine waters British Columbia and poses health risk to both farmed Atlantic Salmon Salmo salar wild Pacific Oncorhynchus spp. At the adult stage, infections can often result severe cutaneous lesions their salmonid hosts. To evaluate compare physiological consequences L. salmonis infections, smolts Sockeye O. nerka were exposed 2 (low), 6 (medium), or 10 (high) female lice/fish. Mean...
DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 133:99-105 (2019) - DOI: https://doi.org/10.3354/dao03335 Host size influences prevalence and severity Kudoa thyrsites (Cnidaria: Myxosporea) infection in Atlantic salmon Salmo salar Simon R. M. Jones*, Amy Long Pacific Biological Station, 3190 Hammond Bay Road, Nanaimo, British Columbia, V9T 6N7, Canada...
Abstract Piscirickettsia salmonis , the aetiological agent of salmonid rickettsial septicaemia (SRS), is a global pathogen wild and cultured marine salmonids. Here, we describe development application reproducible, standardized immersion challenge model to induce clinical SRS in juvenile pink ( Oncorhynchus gorbuscha ), Atlantic Salmo salar ) sockeye salmon O. nerka ). Following 1‐hr 10 5 colony‐forming units/ml, cumulative mortality was 63.2% while 10%. Prevalence levels bacterium kidney...
Abstract The opportunistic examination of factors associated with an outbreak piscirickettsiosis (SRS) is described in Atlantic salmon Salmo salar post‐smolts held open netpen or tanks supplied raw sea water at a research aquarium western Canada. During the outbreak, seawater temperature was significantly higher and salinity lower compared tanks. Mortality began approximately 3 weeks prior to that tanks, cumulative mortality (34%) than (12%). Piscirickettsia salmonis confirmed by qPCR...
Pulmonary hypertension is a chronic vascular disease characterized by pulmonary vasoconstriction and arterial remodeling. remodeling mainly due to small wall thickening lumen occlusion. Previous studies have described intravascular changes in lung sections using histopathology, but few were able obtain fine detailed image of the system. In this study, we used Microfil compounds cast arteries rat model monocrotaline‐induced hypertension. High‐quality images that enabled quantification distal...
Background Salmonid rickettsial septicemia is an emergent and geographically widespread disease of marine-farmed salmonids caused by infection with the water-borne bacterium Piscirickettsia salmonis . Very little known about route, timing, or magnitude bacterial shedding from infected fish. Methodology/principal findings A cohabitation challenge model was used to assess chum Oncorhynchus keta , pink O gorbuscha Atlantic salmon Salmo salar Infections in donor fish were established...
The sole member of the Piscihepevirus genus (family Hepeviridae) is cutthroat trout virus (CTV) but recent metatranscriptomic studies have identified numerous fish hepevirus sequences including CTV-2. In current study, viruses with resembling both CTV and CTV-2 were isolated from salmonids in eastern western Canada. Phylogenetic analysis eight full genomes delineated Canadian isolates into two genotypes (CTV-1 CTV-2) within genus. Hepevirus typically three open reading frames an ORF3...
Abstract It is hypothesized that the frequency of bacterial coldwater disease outbreaks can be reduced through detection aetiologic agent, Flavobacterium psychrophilum , in broodstock followed by culling eggs from heavily infected broodstock. Before a programme instituted, however, it necessary to determine sensitivity and specificity existing assays for F. . In this study, tissue ovarian fluid samples were collected 224 fish at five hatcheries screened using an enzyme‐linked immunosorbent...
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 49:1-14 (2007) - DOI: https://doi.org/10.3354/ame01125 Macroarray analysis of gene expression in a marine pseudotemperate bacteriophage Amy Long1,3, Stacey S. Patterson2, John H. Paul1,* 1College Marine Science, University South Florida, St. Petersburg, Florida 33701, USA 2Center for Environmental Biotechnology,...
Outbreaks of Piscirickettsia salmonis in cultured salmon populations result high mortality and economic loss. An efficacious vaccine against this pathogen is highly desired; however there has been limited success regard. In the current study, Atlantic ( Salmo salar ) smolts were vaccinated by single, double, or quadruple immunization with a commercial oral developed using Chilean isolate P. . Fish then challenged immersion Canadian Significantly greater survival was observed 300 degree-days...
Abstract A well‐functioning 68 year old gentleman presented to our hospital with a macular rash 2 weeks after starting course of Ciprofloxacin. There was rapid progression skin involvement including the mucosa, complicated by pancytopaenia. Toxic Epidermal Necrolysis (TEN) suspected and patient administered intravenous immunoglobulins granulocyte colony stimulating factor. TEN confirmed on biopsy lymphocyte transformation test demonstrated sensitisation The developed multifocal pulmonary...