A5103118245- Biochemical and Molecular Research
- Click Chemistry and Applications
- Cancer Genomics and Diagnostics
- Tuberculosis Research and Epidemiology
- Cancer therapeutics and mechanisms
- Cancer-related Molecular Pathways
- Synthesis and Reactivity of Heterocycles
- Escherichia coli research studies
- Chemical Synthesis and Analysis
- Diatoms and Algae Research
- Antibiotic Resistance in Bacteria
- Lung Cancer Treatments and Mutations
- Probiotics and Fermented Foods
- Sarcoma Diagnosis and Treatment
- Biochemical and Structural Characterization
- Microbial Natural Products and Biosynthesis
- Renal cell carcinoma treatment
- Pain Management and Treatment
- Migraine and Headache Studies
- Bacterial biofilms and quorum sensing
- Enzyme Structure and Function
- Cervical Cancer and HPV Research
- Musculoskeletal pain and rehabilitation
- Synthesis of Indole Derivatives
- Ubiquitin and proteasome pathways
University of Louisville
2022-2024
University of Louisville Hospital
2023
Riverside Community Hospital
2023
University of Minnesota
2018-2020
University of Toledo
2013-2016
Radiographic imaging is the current standard for monitoring progression of tumor-burden and therapeutic resistance in patients with metastatic melanoma. Plasma circulating tumor DNA (ctDNA) has shown promise as a survelience tool, but longitudinal data on dynamics between plasma ctDNA concentrations radiographic lacking. We evaluated relationship measures burden 30 melanoma systemic treatment. In 9 no evidence disease over total 15 time points, were undetectable. 21 burden, was detected 81 %...
Because many cancers harbor mutations that confer resistance to apoptosis, there is a need for therapeutic agents can trigger alternative forms of cell death. Methuosis novel form non-apoptotic death characterized by accumulation vacuoles derived from macropinosomes and endosomes. Previous studies identified an indole-based chalcone, 3-(5-methoxy-2-methylindol-3-yl)-1-(4-pyridinyl)-2-propen-1-one (MOMIPP), induces methuosis in human cancer cells. Herein, we describe the synthesis related...
Mycolic acids are indispensible lipids of Mycobacterium tuberculosis (Mtb), the causative agent (TB), and contribute to distinctive architecture impermeability mycobacterial cell envelope. FadD32 plays a pivotal role in mycolic acid biosynthesis by functionally linking fatty synthase (FAS) polyketide (PKS) biosynthetic pathways. FadD32, acyl-AMP ligase (FAAL), represents one best genetically chemically validated new TB drug targets. We have determined three-dimensional crystal structure Mtb...
Methuosis is a form of nonapoptotic cell death characterized by an accumulation macropinosome-derived vacuoles with eventual loss membrane integrity. Small molecules inducing methuosis could offer significant advantages compared to more traditional anticancer drug therapies that typically rely on apoptosis. Herein we further define the effects chemical substitutions at 2- and 5-indolyl positions our lead compound 3-(5-methoxy-2-methyl-1H-indol-3-yl)-1-(4-pyridinyl)-2-propene-1-one (MOMIPP)....
Tilimycin is an enterotoxin produced by the opportunistic pathogen
Nonribosomal peptide synthetases (NRPSs) are remarkable modular enzymes that synthesize natural products. The condensation (C) domain catalyzes the key amide bond-forming reaction, but structural characterization with bound donor and acceptor substrates has proven elusive. We describe chemoenzymatic synthesis of probes C1 C2 designed to cross-link within active site. These pantetheine contain nonhydrolyzable ketone α,α-difluoroketone isosteres native thioester linkage. Using bimodular NRPS...
Protein degradation is a fundamental process in all living organisms. An important part of this system multisubunit, barrel-shaped protease complex called the proteasome. This enzyme directly responsible for proteolysis ubiquitin- or pup-tagged proteins to smaller peptides. In study, we present series 92 psoralen derivatives, which 15 displayed inhibitory potency against Mycobacterium tuberculosis proteasome low micromolar concentrations. The best inhibitors, i.e., 8, 11, 13 and 15,...
Schematic of ctDNA encapsulation in BioCaRGOS followed by release using invert syringe filtration to eliminate silica interference and enhance the biocompatibility ddPCR is shown.
Background: Endometrial cancer (EC) rates among women are increasing each year. Early diagnostic techniques prone to false positive results and can lead unnecessary invasive follow-up procedures. Long Interspersed Nuclear Element-1 (LINE-1) is a transposable DNA element whose induction by environmental stressors leads carcinogenesis. In this study, we sought establish the value of Open Reading Frame 1 (ORF1), terminal protein product LINE-1 activation, as general malignancy biomarker...