A5074697046- DNA Repair Mechanisms
- Carcinogens and Genotoxicity Assessment
- Plant Genetic and Mutation Studies
- DNA and Nucleic Acid Chemistry
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Viral Infections and Vectors
- Molecular Biology Techniques and Applications
- Bacterial Identification and Susceptibility Testing
- Mosquito-borne diseases and control
- Genetically Modified Organisms Research
- Cancer Research and Treatments
- Viral Infections and Outbreaks Research
- Animal Genetics and Reproduction
- Bacteriophages and microbial interactions
- Radiation Therapy and Dosimetry
- Virology and Viral Diseases
- Forensic and Genetic Research
- Transgenic Plants and Applications
- Cancer Genomics and Diagnostics
- Insect symbiosis and bacterial influences
- Nicotinic Acetylcholine Receptors Study
- Hepatitis B Virus Studies
MRIGlobal
2000-2023
SRI International
1993-2013
Menlo School
1994-2009
Magnetic Resonance Innovations (United States)
2007
University of California, San Francisco
1988-1992
Oak Ridge National Laboratory
1988
University of Tennessee at Knoxville
1988
Bloodstream infection is a serious condition associated with significant morbidity and mortality. The outcome of these infections can be positively affected by the early implementation effective antibiotic therapy based on identification infecting organism genetic markers resistance. In this study, we evaluated microarray-based Verigene Gram-negative blood culture (BC-GN) assay in 8 genus or species targets 6 resistance determinants positive broths. A total 1,847 cultures containing...
The increasing use of nucleic acid-based therapeutics has created a need for new methods determining tissue distribution and levels. Radiolabel may not always be appropriate because acids are easily degraded. Quantitation using the polymerase chain reaction (PCR) advantage that only continuous stretches DNA will amplified. In situ hybridization allows detection specific sequences in histological preparations. We have used quantitative PCR techniques to study pharmacokinetics PGagPol (a...
Rapid, specific, and sensitive identification of microbial pathogens is critical to infectious disease diagnosis surveillance. Classical culture-based methods can be applied a broad range but have long turnaround times. Molecular methods, such as PCR, are time-effective not comprehensive may detect novel strains. Metagenomic shotgun next-generation sequencing (NGS) promises specific characterization any pathogen (viruses, bacteria, fungi, protozoa) in less biased way. Despite its great...
Background Compound A [CF2 = C(CF3)OCH2F], a degradation product of sevoflurane [(CF3)2CHOCH2F], is vinyl ether and may be an alkylating agent. Thus it potential genotoxin. Methods The capacity compound to produce sister chromatid exchanges was measured in Chinese hamster ovary cells with without metabolic activation. Concentrations 11 468 ppm were applied for 2 h, the incubated further 34 h presence bromodeoxyuridine, then colcemid added arrest metaphase. Coded slides examined blindly, 50...
Journal Article Spectrum of mutations produced by specific types restriction enzyme-induced double-strand breaks Get access Richard A. Winegar, Winegar 1 Laboratory Radiobiology and Environmental Health, University CaliforniaSan Francisco, CA 94143–0750, USA 1To whom correspondence should be addressed. Present address: SRI International, 333 Ravenswood Avenue, Box 20540, Menlo Park, 94025, Search for other works this author on: Oxford Academic PubMed Google Scholar Louise H. Lutze, Lutze...
The cytogenetic endpoints sister chromatid exchange (SCE) and chromosome aberrations are widely used as indicators of DNA damage induced by mutagenic carcinogens. Chromosome appear to result directly from double-strand breaks, but the lesion(s) giving rise SCE formation remains unknown. Most compounds that induce SCEs a spectrum lesions in DNA. To investigate role breakage formation, we constructed plasmid gives one specific lesion, staggered-end ("cohesive") break. This plasmid, designated...
Changes in the Earth’s climate and weather continue to impact planet’s ecosystems, including interface of infectious disease agents with their hosts vectors. Environmental disasters, natural human-made activities raise risk factors that indirectly facilitate outbreaks. Subsequently, changes habitat, displaced populations, environmental stresses affect survival species are amplified over time. The recurrence spread vector-borne (e.g., mosquito, tick, aphid) human, animal, plant pathogens new...
Restriction enzymes can be electroporated into mammalian cells, and the induced DNA double-strand breaks lead to aberrations in metaphase chromosomes. Chinese hamster ovary cells were with PstI, which generates 3' cohesive-end breaks, PvuII, blunt-end or XbaI, 5' breaks. Although all three restriction similar numbers of aberrant PvuII was dramatically more effective at inducing both exchange-type deletion-type chromosome aberrations. Our cytogenetic studies also indicated that are active...
The cytogenetic endpoints sister chromatid exchange (SCE) and chromosome aberrations are widely used as indicators of DNA damage induced by mutagenic carcinogens. Chromosome appear to result directly from double-strand breaks, but the lesion(s) giving rise SCE formation remains unknown. Most compounds that induce SCEs a spectrum lesions in DNA. To investigate role breakage formation, we constructed plasmid gives one specific lesion, staggered-end ("cohesive") break. This plasmid, designated...
Coniothyrium glycines, the causal agent of soybean red leaf blotch, is a USDA APHIS-listed Plant Pathogen Select Agent and potential threat to US agriculture. Sequencing C. glycines mt genome revealed circular 98,533-bp molecule with mean GC content 29.01%. It contains twelve mitochondrial genes typically involved in oxidative phosphorylation (atp6, cob, cox1-3, nad1-6, nad4L), one for ribosomal protein (rps3), four hypothetical proteins, each small large subunit RNAs (rns rnl) set 30 tRNAs....
The electroporation of restriction enzymes into mammalian cells results in DNA double-strand breaks that can lead to chromosome aberrations. Four chemicals known interfere with cellular responses damage were investigated for their effects on aberrations induced by AluI and Sau3AI; addition, the number at various times after enzyme treatment was determined pulsed-field gel electrophoresis (PFGE). poly(ADP-ribose) polymerase inhibitor 3-aminobenzamide (3AB) dramatically increased yield...