A5081764030- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Advanced Sensor and Energy Harvesting Materials
- Advanced Materials and Mechanics
- Microtubule and mitosis dynamics
- Microfluidic and Bio-sensing Technologies
- Cancer Cells and Metastasis
- Tissue Engineering and Regenerative Medicine
- Surface Modification and Superhydrophobicity
- Micro and Nano Robotics
- Advanced Fluorescence Microscopy Techniques
- Nanofabrication and Lithography Techniques
- Tendon Structure and Treatment
- Cell Image Analysis Techniques
- Bone Tissue Engineering Materials
- Microbial Inactivation Methods
- Force Microscopy Techniques and Applications
- Caveolin-1 and cellular processes
- Additive Manufacturing and 3D Printing Technologies
- Skin and Cellular Biology Research
- Polymer Surface Interaction Studies
- Melanoma and MAPK Pathways
- Nuclear Structure and Function
- Pluripotent Stem Cells Research
Virginia Tech
2016-2025
Institute of Genomics and Integrative Biology
2024
Discovery Institute
2024
Virginia Tech - Wake Forest University School of Biomedical Engineering & Sciences
2012-2020
Interface (United States)
2012-2014
Carnegie Mellon University
2004-2011
Yale University
2001
This letter proposes a method for fabricating suspended micro-/nanoscale polymer fibers continuously, in which polymeric micro-/nanofibers are formed by drawing and solidification of viscous liquid solution is pumped through glass micropipette. By controlling the parameters, this demonstrated to form networks having amorphous internal structure uniform diameters from micrometers down sub-50-nm different molecular weights polystyrene dissolved xylene.
Polymeric nanofibers are finding increasing number of applications and hold the potential to revolutionize diverse fields such as tissue engineering, smart textiles, sensors, actuators. Aligning producing high aspect ratio fiber arrays (length/diameter > 2 000) in sub-micron nanoscale diameters has been challenging due fragility polymeric materials, thus making it difficult deposit them one dimensional structures functionally interfaced with other systems. Here, we present a pseudo dry...
Extracellular matrix (ECM) is a fibrous natural cell environment, possessing complicated micro- and nanoarchitectures, which provide extracellular signaling cues influence behaviors. Mimicking this three-dimensional microenvironment in vitro challenge developmental disease biology. Here, suspended multilayer hierarchical nanofiber assemblies (diameter from micrometers to less than 100 nm) with accurately controlled fiber orientation spacing are demonstrated as biological scaffolds fabricated...
The state of adhesion bacteria to nanofiber-textured model surfaces is analyzed at a single-cell level. results reveal similarities between the effect topography on bacteria–surface interactions and vesicle–surface interactions. are discussed in context controlling bacterial using nanofibrous topographical features.
Investigating the mechanistic influence of tumor microenvironment on cancer cell migration and membrane blebbing is crucial in understanding eventual arrest metastasis. In this study, we investigate effect suspended aligned nanofibers glioma cytoskeleton, shape, plasma dynamics using a non-electrospinning fiber-manufacturing platform. Cells attached repeatable shapes spindle single fibers, rectangular two parallel fibers polygonal intersecting fibers. Structural stiffness (N m(-1)) (average...
Understanding how cells migrate in fibrous environments is important wound healing, immune function, and cancer progression. A key question fiber orientation network geometry influence cell movement. Here we describe a quantitative, modeling-based approach toward identifying the mechanisms by which geometries having well controlled orientation. Specifically, U251 glioblastoma were seeded onto non-electrospinning Spinneret based tunable engineering parameters substrates that consist of...
Biomechanical cues within tissue microenvironments are critical for maintaining homeostasis, and their disruption can contribute to malignant transformation metastasis. Once transformed, metastatic cancer cells migrate persistently by adapting (plasticity) changes in the local fibrous extracellular matrix, current strategies recapitulate persistent migration rely exclusively on use of aligned geometries. Here, controlled interfiber spacing suspended crosshatch networks nanofibers induces...
Significance When cells heal a wound or invade new area, they coordinate their motion. Coordination is often studied by looking at what happens after pairs of collide. Postcollision, exhibit contact inhibition locomotion—they turn around and crawl away from the point where touched. Past knowledge repolarization on comes studies flat surfaces, unlike in body, which along fibers. We discover that single fibers walk past one another, but with multiple stick to another move as pairs. This...
Polarized fluorescence microscopy is a valuable tool for measuring molecular orientations in biological samples, but techniques recovering three-dimensional and positions of fluorescent ensembles are limited. We report polarized dual-view light-sheet system determining the diffraction-limited distribution dipoles that label structures. share set visualization, histogram, profiling tools interpreting these orientations. model distributions based on polarization-dependent efficiency excitation...
STEP by STEP: A method for depositing customized single and multilayer biomaterial scaffolds (see image) using point-to-point continuous techniques is described. Proliferation migration of mouse C2C12 progenitor cells occurs along the fiber axis on single-layered double-layered orthogonal spreading two layers, right-angle transitions can be seen.
Cell emergence onto damaged or organized fibrous extracellular matrix (ECM) is a crucial precursor to collective cell migration in wound closure and cancer metastasis, respectively. However, there fundamental gap our quantitative understanding of the role local ECM size arrangement emergence–based closure. Here, using ECM-mimicking nanofibers bridging monolayers, we describe method recapitulate quantitatively these vivo behaviors over multispatial (single sheets) temporal (minutes weeks)...
Cytoskeleton-mediated force transmission regulates nucleus morphology. How nuclei shaping occurs in fibrous vivo environments remains poorly understood. Here suspended nanofiber networks of precisely tunable (nm-µm) diameters are used to quantify plasticity mimicking the natural extracellular matrix. Contrary apical cap over cells on 2-dimensional surfaces, cytoskeleton fibers displays a uniform actin network caging nucleus. The role contractility-driven sculpting nuclear shapes is...
The cell migration cycle, well-established in 2D, proceeds with forming new protrusive structures at the membrane and subsequent redistribution of contractile machinery. Three-dimensional (3D) environments are complex composed 1D fibers, fibers shown to recapitulate essential features 3D migration. However, establishment activity contractility fibrous remains partially understood. Here role curvature regulator IRSp53 is examined as a coupler between actin filaments plasma during on single,...
Cell migration is studied with the traditional focus on protrusion-driven cell body displacement, while less known morphodynamics of individual protrusions themselves, especially in fibrous environments mimicking extracellular matrix. Here, using suspended fibers, we report integrative and multiscale abilities to study protrusive behavior independent migration. By manipulating diameter fibers orthogonal directions, constrain along large (2 μm) base solely allowing cells sense, initiate,...
Abstract Aligned extracellular matrix fibers enable fibroblasts to undergo myofibroblastic activation and achieve elongated shapes. Activated are able contract, perpetuating the alignment of these fibers. This poorly understood feedback process is critical in chronic fibrosis conditions, including cancer. Here, using fiber networks that serve as force sensors, we identify “3D perpendicular lateral protrusions” (3D-PLPs) evolve from cell extensions named twines. Twines originate...
Exogenous high-voltage pulses increase cell membrane permeability through a phenomenon known as electroporation. This process may also disrupt the cytoskeleton causing changes in contractility; however, contractile signature of force after electroporation remains unknown. Here, single-cell forces post-electroporation are measured using suspended extracellular matrix-mimicking nanofibers that act sensors. Ten, 100 μs delivered at three voltage magnitudes (500, 1000, and 1500 V) two directions...
Abstract Protrusions at the leading-edge of a cell play an important role in sensing extracellular cues during cellular spreading and motility. Recent studies provided indications that these protrusions wrap (coil) around fibers. However, physics this coiling process, mechanisms drive it, are not well understood. We present combined theoretical experimental study on fibers different geometry. Our model describes membrane produced by curved proteins recruit protrusive forces actin...
Polarized fluorescence microscopy is a valuable tool for measuring molecular orientations, but techniques recovering three-dimensional orientations and positions of fluorescent ensembles are limited. We report polarized dual-view light-sheet system determining the diffraction-limited dipoles that label biological structures, we share set visualization, histogram, profiling tools interpreting these orientations. model our samples, their excitation, detection using coarse-grained...
Study Design. An in vitro biomechanical study. Objectives. To develop and evaluate a new whole cervical spine model that provides to the specimen, vivo-like mechanical characteristics. Summary of Background Data. In studies kinematics, kinetics, trauma using isolated specimens (head–T1 vertebra) have usually applied upward force head, resulting tensile forces, contrary physiological compressive forces present vivo. Further, load-displacement curves never been compared with corresponding vivo...