A5077181569- Bacterial Genetics and Biotechnology
- DNA Repair Mechanisms
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Genetics and Neurodevelopmental Disorders
- Computational Drug Discovery Methods
- Advanced Proteomics Techniques and Applications
- Fungal and yeast genetics research
- Mass Spectrometry Techniques and Applications
- Escherichia coli research studies
- Microbial Natural Products and Biosynthesis
- Cancer therapeutics and mechanisms
- Ion channel regulation and function
- Carcinogens and Genotoxicity Assessment
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neuropharmacology Research
- CRISPR and Genetic Engineering
- Plant Genetic and Mutation Studies
- Steroid Chemistry and Biochemistry
- Microtubule and mitosis dynamics
- Epigenetics and DNA Methylation
Vanderbilt University
2017-2024
Institut Pasteur
2021-2024
Université Paris Cité
2022-2024
Centre National de la Recherche Scientifique
2015-2024
Université d'Orléans
2015-2018
Centre de Biophysique Moléculaire
2015-2018
Abstract The XPA protein functions together with the single-stranded DNA (ssDNA) binding RPA as central scaffold to ensure proper positioning of repair factors in multi-protein nucleotide excision (NER) machinery. We previously determined structure a short motif disordered N-terminus bound RPA32C domain. However, second contact between DNA-binding domain (XPA DBD) and RPA70AB tandem ssDNA-binding domains, which is likely influence orientation on damaged substrate, remains poorly...
Earlier studies have shown that the outer layers of conidial and mycelial cell walls Aspergillus fumigatus are different. In this work, we analyzed polysaccharidome resting wall observed major differences within mycelium wall. Mainly, conidia was characterized by (i) a smaller amount α-(1,3)-glucan chitin; (ii) larger β-(1,3)-glucan, which divided into alkali-insoluble water-soluble fractions, (iii) existence specific mannan with side chains containing galactopyranose, glucose,...
Abstract Replication Protein A (RPA) is a heterotrimeric single stranded DNA-binding protein with essential roles in DNA replication, recombination and repair. Little known about the structure of RPA Archaea, third domain life. By using an integrative structural, biochemical biophysical approach, we extensively characterize from Pyrococcus abyssi presence absence DNA. The obtained X-ray cryo-EM structures reveal that trimerization core interactions promoting clustering on ssDNA are shared...
Native mass spectrometry (MS) encompasses methods to keep noncovalent interactions of biomolecular complexes intact in the gas phase throughout instrument and measure mass-to-charge ratios supramolecular directly spectrometer. Electrospray ionization (ESI) nondenaturing conditions is now an established method characterize systems. Matrix-assisted laser desorption/ionization (MALDI), on other hand, consumes low quantities samples largely tolerates contaminants, making it a priori attractive...
The nucleoid-associated protein HU is involved in numerous DNA transactions and thus essential maintenance bacterial survival. high affinity of for SSBs (single-strand breaks) has suggested its involvement protection, repair recombination. SSB-containing are major intermediates transiently generated by bifunctional N-glycosylases that initiate the BER (base excision repair) pathway. Enzyme kinetics DNA-binding experiments demonstrate enhances 8-oxoguanine-DNA glycosylase activity Fpg...
ABSTRACT Replication Protein A (RPA) plays a pivotal role in DNA replication by coating and protecting exposed single-stranded DNA, acting as molecular hub that recruits additional factors. We demonstrated archaeal RPA hosts winged-helix domain (WH) interacts with two key actors of the replisome: primase (PriSL) replicative polymerase (PolD). Using an integrative structural biology approach, combining nuclear magnetic resonance, X-ray crystallography cryo-electron microscopy, we unveiled how...
Abstract Replication Protein A (RPA) plays a pivotal role in DNA replication by coating and protecting exposed single-stranded DNA, acting as molecular hub that recruits additional factors. We demonstrate archaeal RPA hosts winged-helix domain (WH) interacts with two key actors of the replisome: primase (PriSL) replicative polymerase (PolD). Using an integrative structural biology approach, combining nuclear magnetic resonance, X-ray crystallography cryo-electron microscopy, we unveil how...
ABSTRACT Replication Protein A (RPA) is a heterotrimeric single stranded DNA-binding protein with essential roles in DNA replication, recombination and repair, both eukaryotic archaeal cells. By using an integrative approach that combines three crystal structures, four cryo-EM structures complex single-stranded (ssDNA) of different lengths, we extensively characterized RPA from Pyrococcus abyssi states. These show two features conserved eukaryotes: trimeric core module promotes cooperative...
Epigenetics has received much attention in the past decade. Many insights on epigenetic (dys)regulation diseases have been obtained, and clinical therapies targeting them are place. However, readers of marks lacking enlightenment behind this revolution, it is poorly understood how DNA methylation being read translated to chromatin function cellular responses. Chemical probes methyl-CpG readers, such as binding domain proteins (MBDs), could be used study mechanism. We designed analogues...
Abstract NEIL1 is a DNA glycosylase that recognizes and initiates base excision repair of oxidized bases. The ubiquitous ssDNA binding scaffolding protein replication A (RPA) modulates activity in manner depends on structure. Interaction between RPA has been reported, but the molecular basis this interaction yet to be investigated. Using combination NMR spectroscopy isothermal titration calorimetry (ITC), we show interacts with through two contact points. An RPA32C recruitment domain was...