A5017007035- Food Allergy and Anaphylaxis Research
- Plant tissue culture and regeneration
- Allergic Rhinitis and Sensitization
- Contact Dermatitis and Allergies
- Identification and Quantification in Food
- Chromosomal and Genetic Variations
- Seed Germination and Physiology
- Plant Genetic and Mutation Studies
- Plant Reproductive Biology
- Transgenic Plants and Applications
- Occupational exposure and asthma
- Molecular Biology Techniques and Applications
- Bee Products Chemical Analysis
- Soil Carbon and Nitrogen Dynamics
- CRISPR and Genetic Engineering
- Biosensors and Analytical Detection
- Genetic Mapping and Diversity in Plants and Animals
- Catalysis and Oxidation Reactions
- Catalytic Processes in Materials Science
- Microbial Community Ecology and Physiology
- Wastewater Treatment and Nitrogen Removal
- Bioenergy crop production and management
- Advanced biosensing and bioanalysis techniques
- Plant Pathogens and Resistance
- Forensic and Genetic Research
Universidad Complutense de Madrid
2014-2024
Vrije Universiteit Brussel
1998
A novel electrochemical disposable nucleic acid biosensor for simple, rapid, and specific detection of adulterations with horsemeat is reported in this work. The biosensing platform involves immobilization a 40-mer RNA probe characteristic fragment the mitochondrial DNA D-loop region horse onto surface magnetic microcarriers. In addition, signal amplification was accomplished by using commercial antibody to RNA/DNA duplexes bacterial protein conjugated horseradish peroxidase homopolymer...
Among food allergens, peanut is one of the most critical. This study evaluates allergenic features after combination heat, pressure, and enzymatic digestion under sonication, by immunodetection using serum IgE sensitized patients mass-spectroscopy. In studied population, there was a predominance with sensitization to Ara h 9 (nsLTP) followed seed storage proteins (Sprot, 1, 2, 3, 6). The Sprot showed higher reactivity. enzyme E5 efficient for inducing protein fragmentation reactivity...
The eukaryotic recombinases RAD51 and DMC1 are essential for DNA strand-exchange between homologous chromosomes during meiosis. is also expressed mitosis, mediates recombination (HR) sister chromatids. It has been suggested that might be involved in the switch from intersister chromatid somatic cells to interhomolog meiotic recombination. At meiosis, Arabidopsis Atrad51 null mutant fails synapse extensive chromosome fragmentation. Atdmc1 asynaptic, but this case fragmentation absent. Thus...
Tree nuts confer many health benefits due to their high content of vitamins and antioxidants, they are increasingly consumed in the last few years. Food processing is an important industrial tool modify allergenic properties foods, addition ensuring safety enhancing organoleptic characteristics. The effect pressure, without with heating, on SDS-PAGE immunodetection profile potential proteins (anti-11S, anti-2S anti-LTP) pistachio, cashew, peanut, hazelnut, almond, chestnut was investigated....
The aim of this work was to develop and analytically validate a quantitative RT-PCR method, using novel primer sets designed on Pru du 1, 3, 4, 6 allergen-coding sequences, contrast the sensitivity specificity these probes. temperature and/or pressure processing influence ability detect almond allergen targets also analyzed. All primers allowed specific accurate amplification sequences. assessed by amplifying DNA from almond, different Prunus species other common plant food ingredients....
Peanut (Arachis hypogaea) contains allergenic proteins, which make it harmful to the sensitised population. The presence of peanut in foods must be indicated on label, prevent accidental consumption by allergic In this work, we use chloroplast markers for specific detection real-time PCR (Polymerase Chain Reaction), order increase assay sensitivity. Binary mixtures raw and processed flour wheat were performed at concentrations ranging from 100,000 0.1 mg/kg. DNA isolation peanut, mixtures,...
This work reports the development and application of a disposable amperometric sensor built on magnetic microcarriers coupled to an Express PCR strategy amplify specific DNA fragment chloroplast trnH-psbA. The procedure involves selective capture 68-mer synthetic target (or unmodified products) through sandwich hybridization with RNA probe-modified streptavidin MBs signaling probes, labeled using antibodies heteroduplexes secondary tagged horseradish peroxidase. Amperometric measurements...
Nuts are considered healthy foods due to their high content of nutritional compounds with functional properties. However, the list most allergenic includes tree nuts, and presence must be indicated on food labels. Most nut allergens seed storage proteins, pathogenesis-related (PR) profilins lipid transfer proteins (LTP). Nut characterized by resistance denaturation proteolysis. Food processing has been proposed as method choice alter allergenicity ensure safety improve organoleptic The...
Food allergies to hazelnut represent an important health problem in industrialized countries because of their high prevalence and severity. allergenicity can be changed by several processing procedures since food proteins may undergo modifications which could alter immunoreactivity. High-hydrostatic pressure (HHP) is emerging technology used develop novel high-quality foods. The effect HHP on currently being investigated through changes protein structure. Our aim evaluate the profile...