A5039506098- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
- Advanced Biosensing Techniques and Applications
- Analytical Chemistry and Sensors
- Monoclonal and Polyclonal Antibodies Research
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Food Allergy and Anaphylaxis Research
- Microfluidic and Bio-sensing Technologies
- Micro and Nano Robotics
- Molecular Junctions and Nanostructures
- RNA modifications and cancer
- Allergic Rhinitis and Sensitization
- Identification and Quantification in Food
- Advanced Chemical Sensor Technologies
- Molecular Communication and Nanonetworks
- Pneumonia and Respiratory Infections
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Advanced Nanomaterials in Catalysis
- Advanced Sensor and Energy Harvesting Materials
- Contact Dermatitis and Allergies
- Epigenetics and DNA Methylation
Universidad Complutense de Madrid
2016-2025
Centro de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable
2025
Instituto de Salud Carlos III
2025
Faculty (United Kingdom)
2015-2021
Zero to Three
2014
University of California, San Diego
2010-2012
Centro de Investigaciones Biológicas Margarita Salas
2008-2011
VA Greater Los Angeles Healthcare System
2010
University of California, Los Angeles
2010
Instituto de Catálisis y Petroleoquímica
2003-2008
Abstract Fuel‐free nanomotors are essential for future in‐vivo biomedical transport and drug‐delivery applications. Herein, the first example of directed delivery drug‐loaded magnetic polymeric particles using magnetically driven flexible nanoswimmers is described. It demonstrated that nickel–silver (5–6 μm in length 200 nm diameter) able to micrometer at high speeds more than 10 s −1 (more 0.2 body lengths per revolution dimensionless speed). The fundamental mechanism cargo‐towing ability...
Microrockets detect cancer: A micromachine is used for the in vitro isolation of pancreatic cancer cells complex media. The strategy based on selective binding and transport ability antibody-functionalized microengine rockets: these microrockets selectively recognize surface antigens overexpressed by cells, capture over a preselected path (see picture). Detailed facts importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited...
New template-based self-propelled gold/nickel/polyaniline/platinum (Au/Ni/PANI/Pt) microtubular engines, functionalized with the Concanavalin A (ConA) lectin bioreceptor, are shown to be extremely useful for rapid, real-time isolation of Escherichia coli (E. coli) bacteria from fuel-enhanced environmental, food, and clinical samples. These multifunctional microtube engines combine selective capture E. uptake polymeric drug-carrier particles provide an attractive motion-based theranostics...
A nanomotor-based strategy for rapid single-step intracellular biosensing of a target miRNA, expressed in intact cancer cells, at the single cell level is described. The new concept relies on use ultrasound (US) propelled dye-labeled single-stranded DNA (ssDNA)/graphene-oxide (GO) coated gold nanowires (AuNWs) capable penetrating cells. Once nanomotor internalized into cell, quenched fluorescence signal (produced by π–π interaction between GO and ssDNA) recovered due to displacement...
The transport properties of single-strand DNA probe-modified self-propelling micromachines are exploited for "on-the-fly" hybridization and selective single-step isolation target nucleic acids from "raw" microliter biological samples (serum, urine, crude E. coli lysate, saliva). rapid movement the guided modified microrockets induces fluid convection, which enhances efficiency, thus enabling acid targets untreated samples. integration these autonomous into a lab-on-chip device that provides...
Novel core-shell Fe3O4@poly(dopamine) magnetic nanoparticles were prepared through an in situ self-polymerization method. The hybrid nanomaterial showed average core diameter of 11 ± 3 nm and a polymer thin film thickness 1.8 0.2 nm. employed as solid supports for the covalent immobilization horseradish peroxidase (HRP), resulting biofunctionalized to construct amperometric biosensor H2O2. enzyme high sensitivity 442.14 mA M-1 cm-2, low limit detection 182 nM, wide linear range from 6.0 ×...
Direct and efficient intracellular delivery of enzymes to cytosol holds tremendous therapeutic potential while remaining an unmet technical challenge. Herein, ultrasound (US)-propelled nanomotor approach a high-pH-responsive strategy are reported overcome this challenge using caspase-3 (CASP-3) as model enzyme. Consisting gold nanowire (AuNW) motor with pH-responsive polymer coating, in which the CASP-3 is loaded, resulting protects enzyme from release deactivation prior reaching...
A ternary surface monolayer, consisting of coassembled thiolated capture probe, mercaptohexanol and dithiothreitol, is shown to offer dramatic improvements in the signal-to-noise characteristics electrochemical DNA hybridization biosensors based on common self-assembled monolayers. Remarkably low detection limits down 40 zmol (in 4 μL samples) as well only 1 CFU Escherichia coli per sensor are thus obtained without any additional amplification step connection commonly used horseradish...
We describe here a new strategy for isolating target proteins from complex biological samples based on an aptamer-modified self-propelled microtube engine. For this purpose, thiolated thrombin or mixed thrombin–ATP aptamer (prehybridized with short DNA) was coassembled mercaptohexanol onto the gold surface of these engines. The rapid movement microtransporter resulted in highly selective and capture thrombin, effective discrimination against large excess nontarget proteins. Release captured...
Nanoparticles composed of Prussian Blue, PB, and the cyanometalate structural analogues, CuFe, FeCoFe, FeCo, are examined as inorganic clusters that mimic functions peroxidases. PB acts a superior catalyst for oxidation dopamine to aminochrome by H2O2. The H2O2 in presence is 6-fold faster than CuFe. cluster FeCo does not catalyze aminochrome. most efficient generation chemiluminescence luminol is, however, lacks any catalytic activity toward chemiluminescence. order catalyzed ≫ CuFe >...
MicroRNAs (miRs) have emerged as important clinical biomarkers with both diagnostic and prognostic value for relevant diseases, such cancer. MiRs pose unique challenges detection are currently detected by northern blotting, real-time PCR, microarray techniques. These expensive, complicated, time-consuming techniques not feasible on-site miR determination. In this study, amperometric magnetobiosensors involving RNA-binding viral protein p19 a selective biorecognition element were developed...
A perspective review of recent strategies involving the use nano/microvehicles to address key challenges associated with delivery and (bio)sensing at cellular level is presented.