A5055478208- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
- Photosynthetic Processes and Mechanisms
- Advanced biosensing and bioanalysis techniques
- Electrocatalysts for Energy Conversion
- Biosensors and Analytical Detection
- Analytical Chemistry and Sensors
- Microbial Fuel Cells and Bioremediation
- Metalloenzymes and iron-sulfur proteins
- Advanced battery technologies research
- Conducting polymers and applications
- Photoreceptor and optogenetics research
- Supercapacitor Materials and Fabrication
- Advanced Photocatalysis Techniques
- Enzyme Catalysis and Immobilization
- Molecular Junctions and Nanostructures
- Fuel Cells and Related Materials
- CO2 Reduction Techniques and Catalysts
- MicroRNA in disease regulation
- RNA modifications and cancer
- Gas Sensing Nanomaterials and Sensors
- Copper-based nanomaterials and applications
- Antibiotics Pharmacokinetics and Efficacy
- Analytical chemistry methods development
- Spectroscopy and Quantum Chemical Studies
Universidade Nova de Lisboa
2022-2025
Instituto de Tecnología Química
2024-2025
Ca' Foscari University of Venice
2023
Ruhr University Bochum
2014-2022
Faculty (United Kingdom)
2019-2020
Universidad Complutense de Madrid
2010-2017
Zero to Three
2014
Oxygenic photosynthetic organisms perform solar energy conversion of water and CO2 to O2 sugar at a broad range wavelengths light intensities. These cells also metabolize sugars using respiratory system that functionally overlaps the apparatus. In this study, we describe harvesting photocurrent used for hydrogen production from live cyanobacteria. A non-harmful gentle physical treatment cyanobacterial enables light-driven electron transfer by an endogenous mediator graphite electrode in...
MicroRNAs (miRs) have emerged as important clinical biomarkers with both diagnostic and prognostic value for relevant diseases, such cancer. MiRs pose unique challenges detection are currently detected by northern blotting, real-time PCR, microarray techniques. These expensive, complicated, time-consuming techniques not feasible on-site miR determination. In this study, amperometric magnetobiosensors involving RNA-binding viral protein p19 a selective biorecognition element were developed...
Bioelectrocatalysis provides access to sustainable and highly efficient technological applications. However, several limitations related either the intrinsic properties of biocatalyst or technical difficulties still hamper even prevent integration such devices into technologically relevant large-scale processes. In this Review, we challenge common viewpoint suggesting biology-based catalytic systems as a promising approach for provision stored energy discuss status bioelectrocatalytic...
Abstract The integration of sensitive catalysts in redox matrices opens up the possibility for their protection from deactivating molecules such as O 2 . [FeFe]‐hydrogenases are enzymes catalyzing H oxidation/production which irreversibly deactivated by Therefore, use under aerobic conditions has never been achieved. Integration hydrogenases viologen‐modified hydrogel films allows enzyme to maintain catalytic current oxidation presence , demonstrating a mechanism independent reactivation...
Abstract Hydrogen is one of the most promising alternatives for fossil fuels. However, power output hydrogen/oxygen fuel cells often restricted by mass transport limitations substrate. Here, we present a dual-gas breathing H 2 /air biofuel cell that overcomes these limitations. The equipped with hydrogen-oxidizing redox polymer/hydrogenase gas-breathing bioanode and an oxygen-reducing bilirubin oxidase biocathode (operated in direct electron transfer regime). consists two layer system...
Abstract We propose the very first “Nernstian biosupercapacitor”, a biodevice based on only one redox polymer: poly(vinyl imidazole‐ co ‐allylamine)[Os(bpy) 2 Cl], and two biocatalysts. At bioanode PQQ‐dependent glucose dehydrogenase reduces Os 3+ moieties at polymer to 2+ shifting Nernst potential of /Os couple negative values. Concomitantly, biocathode reduction O by means bilirubin oxidase embedded in same leads oxidation higher Despite use just an open circuit voltage more than 0.45 V...
In pursuance of efficient tools for the local analysis and characterization novel photoelectrocatalytic materials, several SECM-based techniques have been developed, aiming on combined benefit a irradiation analyzed sample microelectrode probe localized electrochemical surface. We present development application scanning photoelectrochemical microscopy (SPECM) laterally resolved materials. Particularly, system was developed n-type semiconductor-based photoanodes water splitting. By using tip...
Abstract The inorganic content and the catalytic performance pose metal‐loaded enzyme nanoflowers as promising candidates for developing bioelectrodes capable of functioning without external addition a redox mediator. However, these protein‐inorganic hybrids have yet to be successfully applied in combination with electrode materials. Herein, synthesis procedure bionanomaterials is reproposed precisely control morphology, composition, this particular protein‐mineral hybrid, formed by glucose...
Abstract Hyperthermophilic (‘superheat-loving’) archaea found in high-temperature environments such as Pyrobaculum aerophilum contain multicopper oxidases (MCOs) with remarkable efficiency for oxidizing cuprous and ferrous ions. In this work, directed evolution was used to expand the substrate specificity of P. McoP organic substrates. Six rounds error-prone PCR DNA shuffling followed by high-throughput screening lead identification a hit variant 220-fold increased ( k cat /K m ) than...
An integrated amperometric biosensor for the determination of lactose is reported. The bioelectrode design based on use a 3-mercaptopropionic acid (MPA) self-assembled monolayer (SAM)-modified gold electrode which enzymes β-galactosidase (β-Gal), glucose oxidase (GOD), peroxidase (HRP) and mediator tetrathiafulvalene (TTF) are coimmobilized by dialysis membrane. β-Gal catalyzes hydrolysis lactose, produced catalytically oxidized to gluconic H2O2, reduced in presence HRP. This enzyme reaction...
In the development of biofuel cells great effort is dedicated to achieving outstanding figures merit, such as high stability, maximum power output, and a large open circuit voltage. Biofuel with immobilized redox mediators, polymers integrated enzymes, show experimentally substantially higher voltage than thermodynamically expected value. Although this phenomenon widely reported in literature, there no comprehensive understanding potential shift, voltages have not been discussed detail,...
Abstract Hydrogenases with Ni- and/or Fe-based active sites are highly hydrogen oxidation catalysts activities similar to those of noble metal catalysts. However, the activity is connected a sensitivity towards high-potential deactivation and oxygen damage. Here we report fully protected polymer multilayer/hydrogenase-based bioanode in which sensitive catalyst from damage by using multilayer architecture. The embedded low-potential (protection deactivation) covered polymer-supported...
Abstract We describe the fabrication of a self‐powered ethanol biosensor comprising β‐NAD + ‐dependent alcohol dehydrogenase (ADH) bioanode and bienzymatic oxidase (AOx) horseradish peroxidase (HRP) biocathode. is regenerated by means specifically designed phenothiazine dye (i. e. toluidine blue, TB) modified redox polymer in which TB was covalently anchored to hexanoic acid tethered poly(4‐vinylpyridine) backbone. The acts as an immobilization matrix for ADH. Using carefully chosen...
The influence of elements Mo and W on the PEC response BiVO<sub>4</sub> based thin films is visualized concerning photocurrent <italic>in situ</italic> detection locally evolved O<sub>2</sub> at a photoabsorber.
We report on a biophotocathode based photosystem 1 (PS1)-Pt nanoparticle complexes integrated in redox hydrogel for photoelectrocatalytic H2 evolution at low overpotential. A poly(vinyl)imidazole Os(bispyridine)2Cl polymer serves as conducting matrix to shuttle the electrons from electrode PS1-Pt embedded within hydrogel. Light induced charge separation results generation of photocurrents (4.8 ± 0.4 μA cm(-2)) when biophotocathodes are exposed anaerobic buffer solutions. Under these...
An oriented photosystem I monolayer with minimised short-circuiting provides anisotropic electron flow, further coupling to a hydrogenase for realising light-induced H<sub>2</sub> evolution.
Abstract Two different D‐dimer disposable amperometric immunosensing designs based on indirect competitive or sandwich formats and the use of carboxylic acid‐modified magnetic beads (COOH‐MBs) screen‐printed carbon electrodes (SPCEs) have been developed compared. In both approaches, resulting modified MBs were magnetically captured surface a SPCE which was used as transducer for electrochemical detection at −0.20 V upon addition H 2 O , hydroquinone (HQ). Both configurations exhibited linear...
Abstract An intrinsic self‐charging biosupercapacitor built on a unique concept for the fabrication of biodevices based redox polymers is presented. The consists high‐potential polymer bioanode and low‐potential biocathode in which potentials electrodes discharged state show an apparent potential mismatch E anode > cathode prevent use device as conventional biofuel cell. Upon charging, are shifted to more positive (cathode) negative (anode) values because change ox ‐to‐ red ratio within...