A5076390224- Pneumonia and Respiratory Infections
- Glycosylation and Glycoproteins Research
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- Microbial Natural Products and Biosynthesis
- Cancer-related Molecular Pathways
- Carbohydrate Chemistry and Synthesis
- Marine Sponges and Natural Products
- Autoimmune Bullous Skin Diseases
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Cancer, Hypoxia, and Metabolism
- Peptidase Inhibition and Analysis
- Bacteriophages and microbial interactions
- Plant Pathogens and Fungal Diseases
- Skin and Cellular Biology Research
- Antimicrobial Resistance in Staphylococcus
- Nutrition and Health in Aging
- Protein Kinase Regulation and GTPase Signaling
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- Cell death mechanisms and regulation
- Nerve injury and regeneration
- RNA and protein synthesis mechanisms
- Dermatological and Skeletal Disorders
Centro de Investigaciones Biológicas Margarita Salas
1992-2020
Consejo Superior de Investigaciones Científicas
1987-2020
Centre for Biomedical Network Research on Rare Diseases
2020
Hospital Universitario de Getafe
2014
Centro de Investigacion Principe Felipe
2008-2009
PharmaMar (Spain)
2002-2006
Universidade da Coruña
2004
GlaxoSmithKline (Spain)
2000
Abstract Thiocoraline is a thiodepsipeptide antitumor compound produced by two actinomycetes Micromonospora sp. ACM2‐092 and ML1, isolated from marine invertebrates (a soft coral mollusc) found of the Indian Ocean coast Mozambique. By using oligoprimers derived nonribosomal peptide synthetase (NRPS) consensus sequences, six PCR fragments containing putative NRPS adenylation domains were amplified chromosome ML1. Insertional inactivation each domain showed that them generated nonproducing...
A 2.9-kilobase Acc I fragment of the DNA pneumococcal bacteriophage Cp-1, containing cpl gene, hybridizes with lytA gene encoding amidase. The nucleotide sequence a muramidase (CPL), has been determined. 3' regions and coding sequences show considerable homology carboxyl-terminal domains deduced amino acid these lysins are quite similar: 73 142 residues identical, 69 substitutions, 55 conservative. Comparisons between CPL, amidase, fungus Chalaropsis sp. (an enzyme that also degrades cell...
Abstract Spinal cord injury (SCI) is a major cause of paralysis. Currently, there are no effective therapies to reverse this disabling condition. The presence ependymal stem/progenitor cells (epSPCs) in the adult spinal suggests that endogenous stem cell-associated mechanisms might be exploited repair lesions. epSPC proliferate after SCI recruited by injured zone, and can modulated innate adaptive immune responses. Here we demonstrate when epSPCs cultured from rats with (ependymal [epSPCi]),...
MicroRNAs (miRs) have emerged as important clinical biomarkers with both diagnostic and prognostic value for relevant diseases, such cancer. MiRs pose unique challenges detection are currently detected by northern blotting, real-time PCR, microarray techniques. These expensive, complicated, time-consuming techniques not feasible on-site miR determination. In this study, amperometric magnetobiosensors involving RNA-binding viral protein p19 a selective biorecognition element were developed...
The first mutant of Streptococcus pneumoniae showing a complete deletion in the lytA gene coding for N-acetylmuramyl-L-alanine amidase has been isolated and characterized. This was previously only autolysin detected this species. shows normal growth rate can be transformed using either chromosomal or plasmid DNA. most remarkable biological consequences absence are formation small chains (six to eight cells) lysis stationary phase growth. In addition, exhibits tolerant response against...
We report that Aplidin, a novel antitumor agent of marine origin presently undergoing Phase II clinical trials, induced growth arrest and apoptosis in human MDA-MB-231 breast cancer cells at nanomolar concentrations. Aplidin specific cellular stress response program, including sustained activation the epidermal factor receptor (EGFR), non-receptor protein-tyrosine kinase Src, serine/threonine kinases JNK p38 MAPK. Aplidin-induced was only partially blocked by general caspase inhibitor...
The biology of the α subunits hypoxia-inducible factors (HIFα) has expanded from their role in angiogenesis to current position self-renewal and differentiation stem cells. results reported this article show discovery FM19G11, a novel chemical entity that inhibits HIFα proteins repress target genes two subunits, various tumor cell lines as well adult embryonic models rodents humans, respectively. FM19G11 at nanomolar range transcriptional protein expression Oct4, Sox2, Nanog, Tgf-α...
Mesenchymal stem cells (MSC) are effective in treating myocardial infarction (MI) and previous reports demonstrated that hypoxia improves MSC self-renewal therapeutics. Considering hypoxia-inducible factor-1 alpha (HIF-1α) is a master regulator of the adaptative response to hypoxia, we hypothesized HIF-1α overexpression could mimic some mechanisms triggered by increase their therapeutic potential without stimulation. Transduction with lentivirus vectors (MSC-HIF) resulted increased cell...
Auranofin is an FDA-approved, gold-containing compound in clinical use for the oral treatment of rheumatoid arthritis and has been recently granted by regulatory authorities due to its antiprotozoal properties.A reprofiling strategy was performed with a Streptococcus pneumoniae phenotypic screen proprietary library compounds, consisting both FDA-approved unapproved bioactive compounds. Two different multiresistant S. strains were employed sepsis mouse model infection. In addition, MRSA...
A pneumococcal recombinant plasmid, pRG2, containing the lytA gene that codes for N ‐acetylmuramoyl‐L‐alanine amidase has been constructed using plasmid pLS1 as a vector. pRG2 was introduced by genetic transformation into mutant of Streptococcus pneumoniae (M31) complete deletion gene. The transformed strain (M51) grew at normal growth rate ‘diplo’ cells and underwent autolysis end exponential phase growth, two properties had lost in deleted M31. M51 lysed very rapidly when were grown...
Abstract The DNA mismatch repair (MMR) system maintains genomic integrity by correcting replication errors: its malfunction causes instability in several tumor types. Hypoxia-inducible factor-1α (HIF1α), the major regulator of processes that occur hypoxia and certain epigenetic events downregulate expression MMR genes cancer cells. However, there is a lack information regarding regulation genetic stability stem cells under hypoxic conditions. downregulated murine human cultured hypoxia,...
We have developed a new single‐step system, using DEAE matrix, to immobilize and/or purify fusion proteins containing the choline‐binding domain of Streptococcus pneumoniae murein hydrolases. constructed choline‐binding‐domain –β‐galactosidase chimera, which can be purified by this procedure and shows high β‐galactosidase activity when immobilized in column. A vector plasmid, pCUZ1, lpp p ‐5/lac promoter as well 13 restriction sites, was facilitate cloning expression gene fusions. This...
Summary Safracin is an antibiotic with anti‐tumour activity produced by Pseudomonas fluorescens A2‐2. The entire safracin synthetic gene cluster spanning 17.5 kb has been identified, cloned and sequenced. comprises 10 open reading frames (ORFs) encoding proteins for three non‐ribosomal peptide synthetases (NRPS), precursor biosynthetic enzymes, two tailoring a resistance protein small hypothetical of unknown function. These genes are organized in divergent operons eight respectively. This...
Controlled delivery of multiple chemotherapeutics can improve the effectiveness treatments and reduce side effects relapses. Here in, we used albumin-stabilized gold nanoclusters modified with doxorubicin SN38 (AuNCs-DS) as combined therapy for cancer. The are conjugated to nanostructures using linkers that release them when exposed different internal stimuli (Glutathione pH). This system has shown potent antitumor activity against breast pancreatic cancer cells. Our studies indicate...
Two cytotoxic alkaloids, discorhabdins L (1) and I (2), were isolated from Latrunculia brevis their structures assigned on the basis of detailed spectroscopic analysis comparison with known metabolites R (5), D (6), B (4). Compounds 1 2 showed strong in vitro cytotoxicity against a panel 14 tumor cell lines.
Abstract A novel magnetobiosensing approach for the rapid, sensitive and selective miR‐21 detection is reported involving use of a specific RNA probe (antimiR‐21), streptavidin‐magnetic beads (Strep‐MBs), siRNA Binding Protein p19 as detector bioreceptor, amperometric with H 2 O /hydroquinone (HQ) system at disposable screen‐printed carbon electrodes. The magnetosensor exhibited dynamic range from 1.4 to 10 nM limit 4.2 fmol synthetic target without any amplification step in 75 min....
FM19G11 up-regulates mammalian target of rapamycin (mTOR)/hypoxia inducible factor-1α (HIF-1α) and PI3K/Akt pathways, which are involved in endothelial function. We evaluated the effects on defective vasodilatation arteries from rats humans investigated mechanisms involved.Effects chronic vivo administration aortic were together with ex treatment mesenteric control insulin-resistant (IRR). Its vasodilator responses penile (HPRAs) corpus cavernosum (HCC) men vasculogenic erectile dysfunction...