A5070534021- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Cancer, Hypoxia, and Metabolism
- Bacterial Infections and Vaccines
- TGF-β signaling in diseases
- Antimicrobial Peptides and Activities
- Oral microbiology and periodontitis research
- Bacteriophages and microbial interactions
- Pneumonia and Respiratory Infections
- Cancer Cells and Metastasis
- Salmonella and Campylobacter epidemiology
- Growth Hormone and Insulin-like Growth Factors
- Antibiotic Resistance in Bacteria
- 3D Printing in Biomedical Research
- Nanofabrication and Lithography Techniques
- Bacterial Identification and Susceptibility Testing
- Liver physiology and pathology
- Biochemical and Structural Characterization
- MicroRNA in disease regulation
- Glaucoma and retinal disorders
- Vascular Procedures and Complications
- Enzyme Production and Characterization
- Polymer Surface Interaction Studies
- Microbial Metabolism and Applications
- Corneal Surgery and Treatments
Instituto de Biomedicina de Sevilla
2024
Universidad de Sevilla
2024
Universidad Publica de Navarra
2012-2022
Navarrabiomed
2020-2022
Complejo Hospitalario de Navarra
2018-2021
Navarre Institute of Health Research
2018-2021
Departamento de Salud
2020
Gobierno de Navarra
2013-2016
Agrobiotechnology Institute
2013-2016
Hospital Central de la Defensa Gómez Ulla
2015
Modification of the biomaterial surface topography is a promising strategy to prevent bacterial adhesion and biofilm formation. In this study, we use direct laser interference patterning (DLIP) modify polystyrene at sub-micrometer scale. The results revealed that three-dimensional micrometer structures have profound impact on adhesion. Thus, line- pillar-like patterns enhanced S. aureus adhesion, whereas complex lamella microtopography reduced in static continuous flow culture conditions....
The biofilm matrix, composed of exopolysaccharides, proteins, nucleic acids and lipids, plays a well-known role as defence structure, protecting bacteria from the host immune system antimicrobial therapy. However, little is known about its responsibility in interaction cells with tissues. Staphylococcus aureus, leading cause biofilm-associated chronic infections, able to develop built on proteinaceous Bap-mediated matrix. Here, we used Bap protein model investigate that components matrix...
ABSTRACT The Staphylococcus aureus biofilm mode of growth is associated with several chronic infections that are very difficult to treat due the recalcitrant nature biofilms clearance by antimicrobials. Accordingly, there an increasing interest in preventing formation S. and developing efficient antibiofilm vaccines. Given fact during a biofilm-associated infection, first primary interface between host bacteria self-produced extracellular matrix, this study we analyzed potential proteins...
The opportunistic pathogen Staphylococcus aureus is responsible for causing infections related to indwelling medical devices, where this able attach and form biofilms. intrinsic properties given by the self-produced extracellular biofilm matrix confer high resistance antibiotics, triggering difficult treat. Therefore, novel antibiofilm strategies targeting components are urgently needed. Biofilm Associated Protein, Bap, expressed staphylococcal species adopts functional amyloid-like...
Auranofin is an FDA-approved, gold-containing compound in clinical use for the oral treatment of rheumatoid arthritis and has been recently granted by regulatory authorities due to its antiprotozoal properties.A reprofiling strategy was performed with a Streptococcus pneumoniae phenotypic screen proprietary library compounds, consisting both FDA-approved unapproved bioactive compounds. Two different multiresistant S. strains were employed sepsis mouse model infection. In addition, MRSA...
In bacteria, adaptation to changes in the environment is mainly controlled through two-component signal transduction systems (TCSs). Most bacteria contain dozens of TCSs, each them responsible for sensing a different range signals and controlling expression repertoire target genes (regulon). Over years, identification regulon by individual TCS has been recurrent question. However, limitations associated with classical approaches used have left our knowledge far from complete. this report,...
ABSTRACT Nontypeable Haemophilus influenzae (NTHi) is a frequent commensal of the human nasopharynx that causes opportunistic infection in immunocompromised individuals. Existing evidence associates lipooligosaccharide (LOS) with disease, but specific and relative contributions NTHi LOS modifications to virulence properties bacterium have not been comprehensively addressed. Using strain 375, an isolate for which detailed structure has determined, we compared systematically set isogenic...
Two-component systems (TCS) are modular signal transduction pathways that allow cells to adapt prevailing environmental conditions by modifying cellular physiology. Staphylococcus aureus has 16 TCSs the diverse microenvironments encountered during its life cycle, including host tissues and implanted medical devices. S. is particularly prone cause infections associated devices, whose surfaces coated serum proteins constitute a particular environment. Identification of involved in adaptation...
Background : The transcription factor pituitary-1 (Pit-1) is mainly expressed in the pituitary gland, where it has critical roles cell differentiation and as a transcriptional for GH prolactin (PRL). It also human extrapituitary tissues (placenta, lymphoid haematopoietic tissues) lines (human breast adenocarcinoma cells, MCF-7). Despite widely suggested of PRL progression proliferative mammary disorders, Pit-1 expression gland not yet been reported. Objective To evaluate and, using MCF-7...
Nontypable Haemophilus influenzae (NTHi) has emerged as an important opportunistic pathogen causing infection in adults suffering obstructive lung diseases. Existing evidence associates chronic by NTHi to the progression of respiratory disease, but specific features associated with persistence have not been comprehensively addressed. To provide clues about adaptive strategies adopted during persistent infection, we compared sequential isolates newly acquired sputa from six patients disease....
Background Inflammation and fibrogenesis are directly related to chronic liver disease progression, including hepatocellular carcinoma (HCC) development. Currently there few therapeutic options available inhibit fibrosis. We have evaluated the hepatoprotective anti-fibrotic potential of orally-administered 5′-methylthioadenosine (MTA) in Mdr2−/− mice, a clinically relevant model sclerosing cholangitis spontaneous biliary fibrosis, followed at later stages by HCC Methodology MTA was...
In the past 15 years, many efforts were made to create functionalized artificial surfaces showing special anti-bacterial and anti-biofouling properties. Thereby, topography of medical relevant materials plays an important role. However, targeted fabrication promising surface structures like hole-, lamella- pyramid-like patterns with feature sizes in sub-micrometer range a one-step process is still challenge. Optical e-beam lithography, molding selfassembly layers show great potential design...
Biofilm engineering has emerged as a controllable way to fabricate living structures with programmable functionalities. The amyloidogenic proteins comprising the biofilms can be engineered create self-assembling extracellular functionalized surfaces. In this regard, facultative amyloids, which play dual role in biofilm formation by acting adhesins their native conformation and matrix scaffolds when they polymerize into amyloid-like fibrillar structures, are interesting candidates. Here, we...
GH expression in mammary tumors has been related to the increase and spreading of cell proliferation. Using MCF-7 human breast adenocarcinoma line, it demonstrated that autocrine GH-stimulated carcinoma proliferation decreased apoptosis rate enhanced spreading. Surprisingly, no data are available about presence Pit-1 (the main pituitary regulator GH) or this line. RT-PCR, Western blot immunohistochemistry, we have both mRNA coding as well protein These could imply may be an adequate target inhibit
Abstract Infections caused by Staphylococcus aureus pose a serious and sometimes fatal health issue. With the aim of exploring novel therapeutic approach, we chose GraXRS, Two-Component System (TCS) that determines bacterial resilience against host innate immune barriers, as an alternative target to disarm S. . Following drug repurposing methodology, taking advantage singular staphylococcal strain lacks whole TCS machinery but one, screened 1.280 off-patent FDA-approved for GraXRS...
Abstract Salmonellosis is the second most common food-borne zoonosis in European Union, with pigs being a major reservoir of this pathogen. Salmonella control pig production requires multiple measures amongst which vaccination may be used to reduce subclinical carriage and shedding prevalent serovars, such as enterica serovar Typhimurium. Live attenuated vaccine strains offer advantages terms enhancing cell mediated immunity allowing inoculation by oral route. However, main failures these...
Salmonellosis is one of the most important bacterial zoonotic diseases transmitted through consumption contaminated food, with chicken and pig related products being key reservoirs infection. Although numerous studies on animal vaccination have been performed in order to reduce Salmonella prevalence, there still a need for an ideal vaccine. Here, aim constructing novel live attenuated vaccine candidate, we firstly analyzed impact absence cyclic-di-GMP (c-di-GMP) virulence. C-di-GMP...
No therapeutic targets and molecular biomarkers are available in cervical cancer (CC) management. In other types, micro-RNA-877-3p (miR-877-3p) has been associated with events relevant for CC development. Thus, we aimed to determine miR-877-3p role CC. levels were examined by quantitative-PCR 117 lesions tumors. Effects on cell proliferation, migration, invasion evaluated upon anti-miR-877-3p transfection. dependent mechanism was comprehensively explored proteomics, dual-luciferase reporter...
OBJECTIVES The objective of this study was to evaluate the feasibility point-of-care testing (POCT) devices for N-terminal pro-B-type natriuretic peptide (NT-proBNP) measurement in prehospital settings, with aim improving speed and accuracy stroke diagnosis, thereby facilitating quicker more effective patient care.
To compare the value of different markers and their combinations with restriction enzyme technique in differentiations penicillinase-producing N. gonorrhoeae (PPNG) strains.17 PPNG strains isolated from symptomatic, untreated male patients urethritis were characterised by antibiotic sensitivity testing, auxotyping, serotyping, plasmid profile, endonuclease fingerprinting (Hind III digestion). Cluster analysis method unweighted pair-group average (UPGMA) linkage was used to calculate...