A5107936387- Gut microbiota and health
- Probiotics and Fermented Foods
- Infant Nutrition and Health
- Neonatal Respiratory Health Research
- Anesthesia and Neurotoxicity Research
- Herpesvirus Infections and Treatments
- Prostate Cancer Treatment and Research
- Infant Development and Preterm Care
- Birth, Development, and Health
- Poxvirus research and outbreaks
- Clostridium difficile and Clostridium perfringens research
- Aerodynamics and Acoustics in Jet Flows
- Heavy Metal Exposure and Toxicity
- Protein Hydrolysis and Bioactive Peptides
- Trace Elements in Health
- Infant Health and Development
- Estrogen and related hormone effects
- Marine Sponges and Natural Products
- Digestive system and related health
- Urinary Tract Infections Management
- Milk Quality and Mastitis in Dairy Cows
- Point processes and geometric inequalities
- Virus-based gene therapy research
- Vehicle Noise and Vibration Control
- Neuropeptides and Animal Physiology
Centro de Biología Molecular Severo Ochoa
2012-2025
Universidad Autónoma de Madrid
1999-2025
Consejo Superior de Investigaciones Científicas
2005-2025
Danone (Netherlands)
2011-2024
Universidad Nacional de Córdoba
2023
Nutricia Research (Netherlands)
2011-2021
Danone Nutricia Research (Netherlands)
2014-2021
Universitat Politècnica de Catalunya
2019-2021
Danone (France)
2021
Catholic University of Ávila
2020
The objective of this work was to elucidate if breast milk contains bifidobacteria and whether they can be transmitted the infant gut through breastfeeding. Twenty-three women their respective infants provided samples feces, respectively, at days 4 7 after birth. Gram-positive catalase-negative isolates from specific media with typical bifidobacterial shapes were identified genus level by F6PPK (fructose-6-phosphate phosphoketolase) assays species 16S rRNA gene sequencing. Bifidobacterial...
Colonization of the infant gut is believed to be critically important for a healthy growth as it influences maturation, metabolic, immune and brain development in early life. Understanding factors that influence this process important, since an altered colonization has been associated with higher risk diseases later Fecal samples were collected from 108 neonates first half year The composition functionality microbiota was characterized by measuring 33 different bacterial taxa qPCR/RT qPCR, 8...
Objectives Bifidobacterium species are one of the major components infant's intestine microbiota. Colonization with bifidobacteria in early infancy is suggested to be important for health later life. However, information remains limited regarding source these microbes. Here, we investigated whether specific strains maternal intestinal flora transmitted their intestine. Materials and Methods Fecal samples were collected from healthy 17 mother infant pairs (Vaginal delivery: 12; Cesarean...
Breast milk is an important factor in the initiation, development, and composition of neonatal gut microbiota. In a previous study, authors isolated lactic acid bacteria from healthy mothers. Since some identified isolates belonged to genus Lactobacillus, objective this work was evaluate probiotic potential 2 Lactobacillus gasseri 1 fermentum strains. Different assays, including survival conditions simulating those existing gastrointestinal tract, production antimicrobial compounds,...
Transport noise is an increasingly prominent feature of the urban environment, making pollution important environmental public health issue. This paper reports on 2001–2003 RANCH project, first cross-national epidemiologic study known to examine exposure-effect relations between aircraft and road traffic exposure reading comprehension. Participants were 2,010 children aged 9–10 years from 89 schools around Amsterdam Schiphol, Madrid Barajas, London Heathrow airports. Data Netherlands, Spain,...
The gastrointestinal tracts of neonates are colonized by bacteria immediately after birth. It has been discussed that the intestinal microbiota includes strains transferred from mothers. Although some studies have indicated possible bacterial transfer mother to newborn, this is first report confirming bifidobacteria at strain level. Here, we investigated mother-to-infant transmission Bifidobacterium longum subsp. genotyping isolates feces mothers before delivery and their infants delivery....
The 16S rRNA gene is conserved across all bacteria and as such routinely targeted in PCR surveys of bacterial diversity. primer design aims to amplify many different sequences from wide a range organisms possible, though there are no suitable 100% regions the gene, leading bias. In gastrointestinal tract, bifidobacteria key genus, but often under-represented We have designed modified, 'bifidobacteria-optimised' universal primers, which we demonstrated detection bifidobacterial sequence...
Antibiotic treatment is common practice in the neonatal ward for prevention and of sepsis, which one leading causes mortality morbidity preterm infants. Although effect antibiotic on microbiota development well recognised, little attention has been paid to duration. We studied short long intravenous administration intestinal Faecal samples from 15 infants (35 ± 1 weeks gestation 2871 260 g birth weight) exposed no, (≤ 3 days) or (≥ 5 with amoxicillin/ceftazidime were collected during first...
The transmission of monkeypox virus occurs through direct contact, but saliva or exhaled droplets and aerosols has not yet been investigated. We aimed to assess the presence DNA infectious in samples from patients infected with virus.We did a cross-sectional study confirmed by PCR who attended two health centres Madrid, Spain. For each patient, we collected saliva, within mask, captured air filtration newly developed nanofiber filters. evaluated viral detection quantitative (qPCR) isolation...
Infectious mastitis is a common condition during lactation and in fact, represents one of the main causes leading to precocious weaning. The number studies dealing with lactational low and, up now, etiological diagnosis frequently made on basis unspecific clinical signs. aim this study was investigate microbial diversity breast milk 20 women employing culture-dependent culture-independent (PCR-DGGE) approaches. Breast samples were cultured different media presence bacteria and/or yeasts,...
Human milk is considered the optimal nutrition for infants and found to contain significant numbers of viable bacteria. The aim study was assess effects a specific synbiotic combination at doses closer bacterial cells present in human milk, on intestinal bifidobacteria proportions (relative abundance), reduction potential pathogens gut physiological conditions. A clinical conducted 290 healthy aged from 6 19 weeks. Infants received either control infant formula or one two investigational...
The ongoing global outbreak of mpox caused by clade IIb viruses has led to more than 100,000 confirmed cases around the world, highlighting urgent need for antiviral research combat current and future outbreaks. Reporter expressing fluorescent proteins monitor viral replication virus spreading in cell culture provide a powerful tool drug screening. In this work, we engineered two recombinant inserting, under control vaccinia early/late promoter 7.5, coding sequence different (EGFP...
Sow milk protects the piglet against infectious diseases through a variety of mechanisms. In this study, presence potentially probiotic lactic acid bacteria in biological fluid was investigated. Milk samples were obtained from 8 sows and total 19 rod-shaped isolates selected for identification assessment their potential. RAPD profiling revealed existence different genetic profiles among them. One representative each profile further characterization they identified as Lactobacillus reuteri,...
Lactobacillus fermentum is a heterofermentative lactic acid bacterium and frequently isolated from mucosal surfaces of healthy humans. CECT 5716 well-characterized probiotic strain human milk and, at present, used in commercial infant formulas. Here, we report the complete annotated genome sequence this strain.