The aim of this study was to investigate selected proteomic markers the metabolic phenotype breast carcinomas as prognostic cancer progression. For purpose, a series 101 and 13 uninvolved samples were examined for quantitative differences in protein expression mitochondrial glycolytic markers. β-subunit H + -ATP synthase (β-F1-ATPase) heat shock 60 (Hsp60), glyceraldehyde-3-phosphate dehydrogenase, pyruvate kinase lactate dehydrogenase identified by immunological techniques. Correlations...

Cold acclimation in woody plants may have special features compared to similar processes herbaceous plants. Recent studies shown that circadian clock behavior the chestnut tree (Castanea sativa) is disrupted by cold temperatures and primary oscillator feedback loop not functional at 4 degrees C or winter. In these conditions, CsTOC1 CsLHY genes are constantly expressed. Here, we show this alteration also affects CsPRR5, CsPRR7 CsPRR9. These homologous corresponding Arabidopsis...

Streptococcus pneumoniae is the main etiological agent of community-acquired pneumonia and a major cause mortality morbidity among children elderly. Genome sequencing several pneumococcal strains revealed valuable information about potential proteins genetic diversity this prevalent human pathogen. However, little known its transcriptional regulation small regulatory noncoding RNAs. In study, we performed deep S. TIGR4 strain RNome to identify RNA candidates expressed in We discovered 1047...

Summary Safracin is an antibiotic with anti‐tumour activity produced by Pseudomonas fluorescens A2‐2. The entire safracin synthetic gene cluster spanning 17.5 kb has been identified, cloned and sequenced. comprises 10 open reading frames (ORFs) encoding proteins for three non‐ribosomal peptide synthetases (NRPS), precursor biosynthetic enzymes, two tailoring a resistance protein small hypothetical of unknown function. These genes are organized in divergent operons eight respectively. This...

Nowadays, cellular bioenergetics has become a central issue of investigation in cancer biology. Recently, the metabolic activity cell been shown to correlate with proteomic index that informs relative mitochondrial cell. Within this new field investigation, we report herein production and characterization high-affinity monoclonal antibodies against proteins “bioenergetic signature” The use recombinant β-F1-ATPase Hsp60 enzymes glycolytic pathway glyceraldehyde-3-phosphate dehydrogenase...

Two elements, the products of genes copG and rnaII, are involved in copy-number control plasmid pLS1. RNA II is synthesized a dosage-dependent manner. Mutations both components have been characterized. To determine regulatory role two genes, we cloned copG, rnaII or elements at various gene dosages into pLS1-compatible plasmids. Assays incompatibility towards wild-type mutant pLS1 plasmids showed that: (i) product, rather than DNA sequence encoding it, responsible for incompatibility, (ii)...

Congenital insensitivity to pain with anhidrosis (CIPA) is a rare autosomal recessive disorder characterized by noxious stimuli and variable intellectual disability (ID) due mutations in the NTRK1 gene encoding NGF receptor TrkA. To get an insight effect of cognitive phenotype we biochemically three TrkA identified children diagnosed CIPA ID. These are located different domains protein; L213P extracellular domain, Δ736 kinase C300stop new mutation causing Spanish teenager. We found that...

ABSTRACT Oligosaccharide synthesis is an important cryoprotection strategy used by woody plants during winter dormancy. At the onset of autumn, starch stored in stem and buds broken down response to shorter days lower temperatures resulting buildup oligosaccharides. Given that enzyme DSP4 necessary for diurnal degradation Arabidopsis leaves, this study was designed address role seasonal process Castanea sativa Mill. The expression pattern CsDSP4 gene cells chestnut found parallel catabolism....

Replication of the streptococcal plasmid pLS1 is controlled by two plasmid‐encoded gene products: repressor protein CopG and antisense RNA, RNA II. Two different mutants in rnaII have been isolated. The 5′‐end levels II synthesized pneumococcal cells harbouring wild‐type or mutant plasmids (affected either genes copG ) were analysed. One exhibited a high‐copy‐number phenotype, whereas an vitro ‐constructed mutation, which affects −10 region promoter, resulted lacking copy‐number phenotype....

The gfp gene from Aequorea victoria, encoding the green fluorescent protein (GFP) has been expressed in Lactococcus lactis subsp. biovar cremoris MG1363, upon construction and introduction of plasmid pLS1GFP into this host. GFP was monitored living cells during growth to evaluate its use molecular physiological studies. Quantification levels by cultures feasible fluorescence spectroscopy. Phase-contrast microscopy allowed us distinguish, mixed cultures, lactococcal expressing GFP. Our...

Streptococcus pneumoniae is a prominent human pathogen responsible for many severe diseases and the leading cause of childhood mortality worldwide. The pneumococcus remarkably adept at colonizing infecting different niches in body, its adaptation to dynamic host environment central aspect pathogenesis. In last decade, increasing findings have evidenced small RNAs (sRNAs) as vital regulators number important processes bacteria. S. pneumoniae, antisense RNA was first discovered pMV158 plasmid...

α-hispanolol (α-H) is a labdane diterpenoid that has been shown to induce apoptosis in several human cancer cells. However, the effect of α-H glioblastoma cells not described. In present work, we have investigated effects on apoptosis, migration and invasion with aim identifying molecular targets underlying its mechanism action. The results revealed showed significant cytotoxicity against glioma cell lines U87 U373 concentration- time-dependent manner. This was higher linked as increased...

Lipid droplets (LD), triglycerides and sterol esters among them, are well known for their capacity as lipid storage organelles. Recently, they have emerged critical cytoplasmic structures involved in numerous biological functions. LD is generated de novo by the cell provides an energy reserve, precursors, protection. Moreover, accumulation can be observed some pathologies obesity, atherosclerosis, or lung diseases. Fluorescence imaging techniques most widely used to visualize cellular...

A modified gfp gene from Aequorea victoria, encoding a variant of the green fluorescent protein (GFP), was subcloned into mobilizable plasmid pMV158. placed under control inducible P M promoter Streptococcus pneumoniae malM, cloned in pLS70. The is regulated by product pneumococcal malR gene, which inactivated growing cells maltose-containing media. By homologous recombination, M–gfp construction integrated host chromosome single copy. In both conditions (single and multiple copies), were...

Streptococcus pneumoniae is a major cause of morbidity and mortality worldwide, about 30% the pneumococcal clinical isolates show type I pili-like structures. These long proteinaceous polymers extending from bacterial surface are encoded by pilus islet 1 play roles in adhesion host colonization. Pili expression bistable controlled transcriptional activator RlrA. In this work, we demonstrate that previously identified small noncoding RNA srn135 also participates regulation. Our findings...

The small transcriptional repressor CopG protein (45 amino acids) encoded by the streptococcal plasmid pMV158 was purified to near homogeneity. Gel filtration chromatography and analytical ultracentrifugation showed that native is a spherical dimer of identical subunits. Circular dichroism measurements indicated consensus average content more than 50% α-helix 10–35% β-strand turns, which compatible with predicted secondary structure protein. exhibited prolonged intracellular half-life, but...

Streptococcus pneumoniae genetic systems designed for isolation of plasmid mutants with copy-up phenotypes have been developed. The target plasmids the pLS1 replicon, and two different strategies followed: (i) selection clones exhibiting augmented resistance to antibiotics, or (ii) obligatory co-existence incompatible plasmids. We isolated 23 increased number copies. All mutations corresponded four alleles copG gene pLS1. These could be used other

Plasmid pMV158 encodes a 45 amino acid transcriptional repressor, CopG, which is involved in copy number control. A new procedure for overproduction and purification of the protein has been developed. The CopG thus obtained retained its ability to specifically bind DNA repress own promoter. Purified crystallized using sitting‐drop vapor diffusion method. crystals, belonging orthorhombic space group C222 1 (cell constants =67.2 Å, b =102.5 c =40.2 Å), were from solution containing...

Abstract Background Pulmonary Langerhans cell histiocytosis (PLCH) is a rare interstitial lung disease (ILD) associated with smoking, whose definitive diagnosis requires the exclusion of other forms ILD and compatible surgical biopsy. Bronchoalveolar lavage (BAL) commonly proposed for ILD, including PLCH, but diagnostic value this technique limited. Here, we have analyzed levels panel cytokines chemokines in BAL from PLCH patients, order to identify distinct immune profile discriminate...

Skin overexposure to UVR leads photodamage (sunburn, photoaging, immune suppression and skin cancer). Cells culture models have been mostly used perform studies of radiation cytotoxicity; however, the response cells under conditions does not reflect a whole organism. Zebrafish ( Danio rerio ) has increased its importance as vertebrate model both for toxicology assays screening therapeutic agents, due transparency, fast development easiness handle. Using this zebrafish model, new...