A5033717367- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
- Analytical Chemistry and Sensors
- DNA and Nucleic Acid Chemistry
- Analytical chemistry methods development
- Monoclonal and Polyclonal Antibodies Research
- Conducting polymers and applications
- RNA and protein synthesis mechanisms
- Celiac Disease Research and Management
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Genetically Modified Organisms Research
- Advanced Biosensing Techniques and Applications
- Glycosylation and Glycoproteins Research
- Viral Infections and Immunology Research
- Cancer-related molecular mechanisms research
- Advanced Proteomics Techniques and Applications
- Identification and Quantification in Food
- Ionic liquids properties and applications
- Molecular Biology Techniques and Applications
- Bacteriophages and microbial interactions
- SARS-CoV-2 detection and testing
- Advanced Chemical Sensor Technologies
Universidad de Oviedo
2016-2025
Instituto de Investigación Sanitaria del Principado de Asturias
2018-2025
Central University Hospital of Asturias
2019-2023
University of California, San Diego
2011-2012
Cornell University
2005
Universidad Nacional del Sur
2000
Microrockets detect cancer: A micromachine is used for the in vitro isolation of pancreatic cancer cells complex media. The strategy based on selective binding and transport ability antibody-functionalized microengine rockets: these microrockets selectively recognize surface antigens overexpressed by cells, capture over a preselected path (see picture). Detailed facts importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited...
Abstract This review deals with the use of pyridine‐nucleotide dependent dehydrogenase enzymes in amperometric biosensor design. The electrochemistry nicotinamide coenzymes, their mechanism and challenges associated direct oxidation on solid electrodes are discussed. In addition, a survey different solutions proposed literature for addressing these problems some applications presented.
Abstract A major societal challenge is the development of necessary tools for early diagnosis diseases such as cancer and sepsis. Consequently, there a concerted push to develop low-cost non-invasive methods analysis with high sensitivity selectivity. notable trend highly sensitive that are not only amenable point-of-care ( POC ) testing, but also wearable devices allowing continuous monitoring biomarkers. In this context, test detection promising biomarker, protein Interleukin-6 (IL-6),...
The first label-free modified RNA-aptasensor for the detection of small molecules in biological samples is presented. A competitive displacement assay was applied to aminoglycoside neomycin B whole milk using a fully 2'-O-methylated RNA aptamer with faradaic impedance spectroscopic (FIS) detection. Neomycin solution displaces from its complex SAM-immobilized B. reusable aptasensor capable discriminating paromomycin, which differs it substitution single amine group hydroxyl one. We show that...
An electrochemical genosensor for the detection of nucleic acid sequences specific Legionella pneumophila is reported. immobilized thiolated hairpin probe combined with a sandwich-type hybridization assay, using biotin as tracer in signaling probe, and streptavidin-alkaline phosphatase reporter molecule. The activity enzyme was voltammetrically determined by measuring amount 1-naphthol generated after 2 min enzymatic dephosphorylation 1-naphthyl phosphate. sensor allows discrimination...
Krebsdetektion mit Mikroraketen: Eine Mikromaschine wird zur In-vitro-Isolierung von Krebszellen der Bauchspeicheldrüse in komplexen Medien verwendet. Die Strategie basiert auf dem selektiven Anbinden und Transportvermögen Antikörper-funktionalisierten Mikromotoren angetrieben Raketen: Diese Mikroraketen erkennen selektiv Oberflächenantigene, die überexprimiert werden, fangen transportieren einem vorgegebenen Pfad (siehe Bild). Detailed facts of importance to specialist readers are published...
Celiac disease represents a significant public health problem in large parts of the world. A major hurdle effective management by celiac sufferers is sensitivity current available methods for assessing gluten contents food. In response, we report highly sensitive approach analysis using aptamers as specific receptors. Gliadins, fraction proteins, are main constituent responsible triggering disease. However, they hydrophobic and molecules, regarded difficult targets vitro evolution without...
The shortage of specific glycan recognition reagents has proven a significant hurdle in the development assays to detect altered glycoforms associated with cancer. Here, carbohydrate-binding aptamer originally selected against moiety prostate-specific antigen (PSA) is used as lectin-mimicking reagent. As first proof-of-principle, this been applied develop sandwich-type electrochemical biosensor for detection serum amyloid P (SAP) component, glycosylated protein whose increased sialylation...
Several routes have been developed to prepare magnetic core–shell Fe3O4@Au nanoparticles (MNPs). However, only highly monodisperse MNPs are suitable for analytical applications. Herein, we describe the detection of GMO through use gold-coated with fine-tuned properties as platforms. The were prepared a procedure that involves preparation Fe3O4 cores by thermal decomposition and their coating reduction gold precursor. Different Fe3O4:Au precursor molar ratios (1:1; 1:4; 1:7) tested on...
Viruses are infectious agents that can only replicate inside cells. Tests available to make an early and effective diagnosis of viral diseases mainly based on PCR, the gold-standard method, or in detection virus antigens antibodies against them. The need for rapid diagnostic tests postulates aptamers as alternative antibodies. Aptamers short single-stranded nucleic acid molecules selected through vitro process called SELEX. They show numerous advantages over antibodies, such greater...