A5059397586- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Muscle metabolism and nutrition
- Regulation of Appetite and Obesity
- Metabolism and Genetic Disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Biochemical Analysis and Sensing Techniques
- Hormonal and reproductive studies
- Fatty Acid Research and Health
- Diet, Metabolism, and Disease
- Birth, Development, and Health
- Animal Nutrition and Physiology
- Peroxisome Proliferator-Activated Receptors
- Biochemical effects in animals
- Clinical Nutrition and Gastroenterology
- Trace Elements in Health
- Antioxidant Activity and Oxidative Stress
- Metabolomics and Mass Spectrometry Studies
- Pharmacology and Obesity Treatment
- Hormonal Regulation and Hypertension
- Heavy Metal Exposure and Toxicity
- Cholesterol and Lipid Metabolism
- Estrogen and related hormone effects
- Thermoregulation and physiological responses
- Metabolism, Diabetes, and Cancer
Universitat de Barcelona
2015-2024
Bellvitge University Hospital
2018-2023
Sony Computer Science Laboratories
2023
Institut d'Investigació Biomédica de Bellvitge
2023
New York University Press
2022
Cambridge University Press
2022
Centro de Investigación Biomédica en Red
2014-2021
Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition
2008-2020
Instituto de Salud Carlos III
2007-2020
Clínica Diagonal
2020
White adipose tissue (WAT) produces large amounts of lactate and glycerol from glucose. We used mature epididymal adipocytes to analyse the relative importance glycolytic versus lipogenic in devoid external stimuli. Cells were incubated (24/48 h) with 7/14 mM glucose; half wells contained 14C-glucose. analysed glucose label fate, medium metabolites, expression key genes coding for proteins controlling metabolism. The effects initial levels small, but time incubation increased cell activity...
White adipose tissue (WAT) produces lactate in significant amount from circulating glucose, especially obesity;Under normoxia, 3T3L1 cells secrete large quantities of to the medium, again at expense glucose and proportionally its levels. Most was converted with only part it being used synthesize fat. Cultured adipocytes were largely anaerobic, but this not a Warburg-like process. It is speculated that massive production lactate, process defense adipocyte, dispose excess glucose. This way,...
Female and male adult Wistar rats were fed standard chow or a simplified cafeteria diet for one month. Then, the killed white adipose tissue (WAT) in four sites: perigonadal, retroperitoneal, mesenteric subcutaneous (inguinal) sampled frozen. The complete WAT weight each site was measured. Gene expression analysis of key lipid glucose metabolism enzymes analyzed, as well plasma lactate activity dehydrogenase. Lactate gradients between estimated. influence sex (and indirectly mass) on levels...
The effects of food deprivation for up to 24 hours on plasma metabolic parameters in the rat have been studied. Liver dry weight and glycogen content dropped significantly from 3 onwards. Total muscle supplied about as much glycosyl residues or precursors did liver. Plasma glucose, urea, lactate total essential amino acids decreased fasting Glycerol, free fatty acids, beta-hydroxybutyrate acetoacetate showed significant increases with fasting. Alanine, serine, arginine, threonine, aspartate...
Plasma amino acid concentrations were determined in virgin female rats, pregnant rats (12 and 21 days after impregnation) 21-day foetuses. The total concentration plasma decreases significantly with pregnancy, being lower at 12 than days. Alanine, glutamine+glutamate other 'gluconeogenic' acids decrease dramatically by mid-term, but regain their original the end of pregnancy. With most acids, mainly essential ones, trend is towards which are maintained throughout These data agree known...
Sex hormone-binding globulin (SHBG) binds and transports testosterone estradiol in plasma. The possibility that SHBG is a mixture of transporting proteins has been postulated. We analyzed parallel the effects obesity status on levels binding capacity circulating their relationship with estradiol.Anthropometric measures plasma were obtained from apparently healthy young (i.e. 35 ± 7 years) premenopausal women (n = 32) men 30), normal weight (BMI >30 kg/m2).SHBG protein (Western blot), as well...
Hematological values of six species galliformes have been determined. The shown are homogeneous.
The metabolic syndrome is basically a maturity-onset disease. Typically, its manifestations begin to flourish years after the initial dietary or environmental aggression began. Since most hormonal, defense responses are practically immediate, procrastinated response don't seem justified. Only in childhood, damages of appear with minimal delay. Sex affects incidence syndrome, but this more an effect timing than absolute gender differences, females holding better males up menopause, when...
Cultured adipocytes (3T3-L1) produce large amounts of 3C fragments; largely lactate, depending on medium glucose levels. Increased glycolysis has been observed also in vivo different sites rat white adipose tissue. We investigated whether fructose can substitute as source and, especially the glycerol released to was lipolytic or glycolytic origin. Fructose conversion lactate and lower than that glucose. The fast exhaustion unrelated significant changes lipid storage. inhibited a higher...
Background Food selection and ingestion both in humans rodents, often is a critical factor determining excess energy intake its related disorders. Methods Two different concepts of high-fat diets were tested for their obesogenic effects rats; cases, lipids constituted about 40% intake. The main difference with controls fed standard lab chow, was, precisely, the lipid content. Cafeteria (K) self-selected devised to be desirable rats, mainly because diverse mix tastes, particularly salty...
The net uptake/release of glucose, lactate and amino acids from the bloodstream by interscapular brown adipose tissue control, cold-exposed cold-acclimated rats was estimated measurement arteriovenous differences in their concentrations. In control animals contributed little to overall energetic needs tissue; glucose uptake more than compensated efflux. Cold-exposure resulted an enhancement acid utilization uptake, with high There a glycine proline efflux that partly positive nitrogen...
Glutamine synthetase activity in several rat tissues has been measured. Liver contains the highest specific followed by stomach, brain, kidneys, intestine, skin, adipose tissue and striated muscle - that had lowest both with regard to tissue, protein DNA weight. Per unit of animal weight, liver contain similar activities. 24 Hours fasting induced a significant decrease liver, intestine skin glutamine synthetase, compensated an increase activity. During fasting, splanchnic is lowered...
Current methodology of gene expression analysis limits the possibilities comparison between cells/tissues organs in which cell size and/or number changes as a consequence study (e.g. starvation). A method relating abundance specific mRNA copies per may allow direct or different changing physiological conditions.With selected genes, we analysed relationship bases and fluorescence recorded at present level using cDNA standards. lineal was found final length transcript. The constants this...
Adipose tissue (AT) is distributed as large differentiated masses, and smaller depots covering vessels, organs, well interspersed within them. The differences between types size of cells makes AT one the most disperse complex organs. Lipid storage partly shared by other tissues such muscle liver. We intended to obtain an approximate estimation lipid reserves stored outside main fat depots. Both male female rats were made overweight 4-weeks feeding a cafeteria diet. Total content was analyzed...