A5006188900- Autophagy in Disease and Therapy
- Historical, Literary, and Cultural Studies
- Medieval Literature and History
- Viral Infections and Immunology Research
- Asthma and respiratory diseases
- RNA regulation and disease
- Cytomegalovirus and herpesvirus research
- Toxoplasma gondii Research Studies
- Mosquito-borne diseases and control
- Extracellular vesicles in disease
- RNA modifications and cancer
- Linguistics and language evolution
- Microtubule and mitosis dynamics
- Sirtuins and Resveratrol in Medicine
- Viral gastroenteritis research and epidemiology
- Plant responses to water stress
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Synthesis of Organic Compounds
- Respiratory viral infections research
- Lipid metabolism and biosynthesis
- Plant Virus Research Studies
- Immune Response and Inflammation
- Historical and Archaeological Studies
- Animal Disease Management and Epidemiology
Stanford University
2004-2025
University of Maryland, Baltimore
2016-2024
The University of Texas Health Science Center at Houston
2017-2024
Arizona State University
2023
Medical College of Wisconsin
2008-2015
University of Michigan
2012
UiT The Arctic University of Norway
2012
Washtenaw Community College
2012
Fairchild Semiconductor (United States)
2009
Eli Lilly (United States)
1992-2000
Infection of human cells with poliovirus induces the proliferation double-membraned cytoplasmic vesicles whose surfaces are used as sites viral RNA replication and origin is unknown. Here, we show that several hallmarks cellular autophagosomes can be identified in poliovirus-induced vesicles, including colocalization LAMP1 LC3, homolog Saccharomyces cerevisiae Atg8p, staining fluorophore monodansylcadaverine followed by fixation. Colocalization LC3 was observed early replicative cycle,...
We have used multiplexed high-throughput sequencing to characterize changes in small RNA populations that occur during viral infection animal cells. Small RNA-based mechanisms such as interference (RNAi) been shown plant and invertebrate systems play a key role host responses infection. Although homologs of the RNAi effector pathways are present mammalian cells, can launch an RNAi-mediated degradation experimentally targeted mRNAs, any for host-virus interactions remains be characterized....
A fine structure study of the phragmoplast and developing cell plate has been made on glutaraldehyde-osmium tetroxide-fixed, dividing, cultured cells liquid endosperm Haemanthus katherinae Baker. The arises between telophase nuclei, usually in association with a remnant strand spindle elements, consists an accumulation microtubules oriented at right angles to plane future plate. microtubules, which are 200–240 diameter, occur small clusters spaced approximately 0.2–0.3 µ intervals along...
The distribution of membrane-associated calcium has been determined at various stages mitosis in Haemanthus endosperm cells with the fluorescent chelate probe chlorotetracycline (CTC). CTC fluorescence two components: punctate, because mitochondrial and plastid membrane-Ca++; diffuse, primarily Ca++ associated endoplasmic reticulum membranes. Punctate assumes a polar throughout mitosis. Cones diffuse chromosomse-to-pole regions metaphase spindle appear to coincide kinetochore fibers; during...
The autophagic pathway acts as part of the immune response against a variety pathogens. However, several pathogens subvert signaling to promote their own replication. In many cases it has been demonstrated that these inhibit or delay degradative aspect autophagy. Here, using poliovirus model virus, we report for first time bona fide degradation occurring during infection with virus whose replication is promoted by We found this not required vesicular acidification, which in case autophagy...
ABSTRACT The mitotic inhibitor isopropyl N-phenylcarbamate (IPC) effects changes in the ultrastructure of dividing endosperm cells Haemanthus katherinae. In treated with 10 p.p.m. IPC for 0·5–2 h microtubules lose their parallel alignment and become oriented radial arrays. These arrays are interpreted as micropoles spindle apparatus thought to be focal points chromosomes they aggregate into micronuclei. Thus, has a markedly different effect on structure than colchicine, which is observed at...
Tobacco is one of the most susceptible crop plants to injury from flooding or saturation soil with water. It really exhibits two types reaction flooding, immediate but temporary wilting accompanying and severe permanent caused by longer periods flooding. If suddenly saturated a downpour rain sun later shines 'bright hot, sudden leaves, often termed flopping tobacco growers, sometimes occurs. This occurs where drainage slow remains for at least few hours ?fter rain. High air temperatures...
Highlights•Enterovirus D68 (EV-D68) induces and benefits from autophagy•The autophagic SNARE SNAP29 promotes EV-D68 in early infection but is later cleaved•The orphan SNAP47 infection•SNAP47 plays a major role autophagy replicationSummaryEnterovirus medically important respiratory plus-strand RNA virus of children that has been linked to acute flaccid myelitis. We have determined signaling membrane formation. Autophagy, homeostatic degradative process breaks down protein aggregates damaged...
ABSTRACT Cellular autophagy, a process that directs cytosolic contents to the endosomal and lysosomal pathways via formation of double-membraned vesicles, is crucial aspect innate immunity many intracellular pathogens. However, evidence accumulating certain RNA viruses, such as poliovirus, subvert this pathway facilitate viral growth. The autophagosome-like membranes induced during infection with wild-type poliovirus were found be, unlike cellular autophagosomes, relatively immobile. Their...
Structural polarities of mitotic spindle microtubules in the plant Haemanthus katherinae have been studied by lysing endosperm cells solutions neurotubulin under conditions that will decorate cellular with curved sheets tubulin protofilaments. Microtubule polarity was observed at several positions each cell cutting serial thin sections perpendicular to axis. The majority present a metaphase or anaphase half-spindle are oriented their fast-growing "plus" ends distal polar area. Near and up...
ABSTRACT The newly formed generative cell of the pollen grain African blood lily is spheroidal after its detachment from wall. Plastids are almost always excluded cell, while dictyosomes, mitochondria, ribosomes, endoplasmic reticulum, and micro-tubules present throughout development cell. During time that spheroidal, microtubules found scattered randomly cytoplasm. subsequently elongates concurrently an oriented system appears along wall aligned with their long axes parallel to axis This...
In humans, loss of TBC1D20 (TBC1 domain family, member 20) protein function causes Warburg Micro syndrome 4 (WARBM4), an autosomal recessive disorder characterized by congenital eye, brain, and genital abnormalities. TBC1D20-deficient mice exhibit ocular abnormalities male infertility. is a ubiquitously expressed the family GTPase-activating proteins (GAPs) that increase intrinsically slow GTP-hydrolysis rate small RAB-GTPases when bound to GTP. Biochemical studies have established as GAP...
We have employed a series of permeant, nontoxic, fluorescent probes to detect changes in ionic conditions within the mitotic apparatus living endosperm cells Haemanthus during transition from metaphase anaphase. Fluorescence emission intensity measurements spindle for chlorotetracycline (CTC) decline before onset anaphase, indicating reduction amount membrane-associated Ca2+ and suggesting an efflux membrane compartments into spindle. Subsequent we observe increases fluorescence with both...