A5065713218- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Radiation Therapy and Dosimetry
- Semiconductor Lasers and Optical Devices
- Photonic and Optical Devices
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- Retinal Development and Disorders
- Animal Virus Infections Studies
- Effects of Radiation Exposure
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Viral Infectious Diseases and Gene Expression in Insects
- Visual perception and processing mechanisms
- Synthesis and properties of polymers
- Boron Compounds in Chemistry
- interferon and immune responses
- Nuclear Physics and Applications
- Estrogen and related hormone effects
- Cancer, Hypoxia, and Metabolism
- DNA Repair Mechanisms
- RNA Research and Splicing
- Plant Virus Research Studies
- Radiopharmaceutical Chemistry and Applications
- Glutathione Transferases and Polymorphisms
Tokyo Metropolitan Institute of Medical Science
2011-2025
Hyogo University of Teacher Education
2024
International University of Health and Welfare
2024
Meiji Pharmaceutical University
2015-2017
National Institutes for Quantum Science and Technology
2010-2017
Fuchu Hospital
2002-2007
National Institute of Advanced Industrial Science and Technology
2007
Shimane University
2005
Seikei University
2005
NTT (Japan)
1990-2003
A cDNA clone of rat uterus estrogen receptor (ER) has been isolated and sequenced. This contains a complete open reading frame encoding 600 amino acid residues which is 5 11 acids larger than the corresponding molecules human chicken, respectively. The molecular weight this protein calculated to be 67,029. When was ligated pSV2 vector transfected into COS7 cells, produced that had same affinity as ER. sequence shows 88% homology with ER; 528 are identical 14 conservative substitutions....
We have used multiplexed high-throughput sequencing to characterize changes in small RNA populations that occur during viral infection animal cells. Small RNA-based mechanisms such as interference (RNAi) been shown plant and invertebrate systems play a key role host responses infection. Although homologs of the RNAi effector pathways are present mammalian cells, can launch an RNAi-mediated degradation experimentally targeted mRNAs, any for host-virus interactions remains be characterized....
Poliovirus-sensitive transgenic mice were produced by introducing the human gene encoding cellular receptors for poliovirus into mouse genome. Expression of receptor mRNAs in tissues was analyzed using RNA blot hybridization and polymerase chain reaction. The is expressed many just as humans. are susceptible to all three serotypes, inoculated with show clinical symptoms similar those observed humans monkeys. Rabbit antipoliovirus serum detects antigens mainly motor neurons anterior horn...
ABSTRACT Poliovirus selectively replicates in neurons the spinal cord and brainstem, although poliovirus receptor (PVR) expression is observed both target nontarget tissues humans transgenic mice expressing human PVR (PVR-transgenic mice). We assessed role of alpha/beta interferon (IFN) determining tissue tropism by comparing pathogenesis virulent Mahoney strain PVR-transgenic deficient IFN gene (PVR-transgenic/ Ifnar knockout PVR-transgenic/ showed increased susceptibility to poliovirus....
To examine the functional relationship between distinct cis-active elements within distal enhancer region of rat PRL gene, we have used deletional and mutational analysis that in transient transfection studies GH3 pituitary tumor cells. Results from these demonstrate containing Pit-1- binding sites is critical not only for activity response to cAMP, but also estradiol. An interaction estrogen receptor with factors conferring basal suggested by a mutant which element was converted palindromic...
Human enterovirus species A (HEV-A) consists of at least 16 members different serotypes that are known to be the causative agents hand, foot, and mouth disease (HFMD), herpangina, other diseases, such as respiratory polio-like flaccid paralysis. Enterovirus 71 (EV71) coxsackievirus A16 (CVA16) major HFMD. CVA5, CVA6, CVA10, CVA12 mainly cause herpangina or occasionally involved with sporadic cases We have previously shown human scavenger receptor class B, member 2 (SCARB2) is a cellular for...
Enterovirus 71 (EV71) typically causes mild hand-foot-and-mouth disease in children, but it can also cause severe neurological disease. Recently, epidemic outbreaks of EV71 with significant mortality have been reported the Asia-Pacific region, and infection has become a serious public health concern worldwide. However, there is little information available concerning neuropathogenesis, no vaccines or anti-EV71 drugs developed. Previous studies this used monkeys neonatal mice that are...
In response to stress, cells induce ribonucleoprotein aggregates, termed stress granules (SGs). SGs are transient loci containing translation-stalled mRNA, which is eventually degraded or recycled for translation. Infection of some viruses, including influenza A virus with a deletion nonstructural protein 1 (IAVΔNS1), induces SG-like aggregates. Previously, we showed that IAVΔNS1-induced required efficient induction type I interferon (IFN). Here, investigated SG formation by different...
ABSTRACT Enterovirus 71 (EV71) is responsible for frequent large-scale outbreaks of hand, foot, and mouth disease worldwide represent a major etiological agent severe, sometimes fatal neurological disease. EV71 variants have been classified into three genogroups (GgA, GgB, GgC), the latter two are further subdivided subgenogroups B1 to B5 C1 C5. To investigate dual roles recombination evolution in epidemiology transmission worldwide, we performed genetic analysis isolates ( n = 308)...
ABSTRACT Human scavenger receptor class B, member 2 (SCARB2), and P-selectin glycoprotein ligand-1 (PSGL1) have been identified to be the cellular receptors for enterovirus 71 (EV71). We compared EV71 infection efficiencies of mouse L cells that expressed SCARB2 (L-SCARB2) PSGL1 (L-PSGL1) abilities bind virus. L-SCARB2 bound a reduced amount L-PSGL1 cells. However, could infect more efficiently than The results suggested difference in binding capacities two was not sole determinant...
A number of mutant cDNAs the human poliovirus receptor were constructed to identify essential regions molecule as receptor. All carrying sequence coding for entire N-terminal immunoglobulin-like domain (domain I) confer permissiveness mouse L cells, but a cDNA lacking I does not. The transformants permissive able bind virus and also recognized by monoclonal antibody D171, which competes with cellular These results strongly suggest that binding site resides in Mutant encoding intracellular...
ABSTRACT RIG-I-like receptors and Toll-like (TLRs) play important roles in the recognition of viral infections. However, how these molecules contribute to defense against poliovirus (PV) infection remains unclear. We characterized sensors PV transgenic mice expressing receptor. observed that alpha/beta interferon (IFN-α/β) production response occurred an MDA5-dependent but RIG-I-independent manner primary cultured kidney cells vitro . These results suggest that, similar RNA other...
We previously identified human scavenger receptor class B, member 2 (SCARB2), as a cellular for enterovirus 71 (EV71). Expression of SCARB2 (hSCARB2) permitted mouse L929 cells to efficiently bind virions and produce both viral proteins progeny viruses upon EV71 infection. Mouse Scarb2 (mScarb2) exhibited 85.8% amino acid identity 99.9% similarity hSCARB2. The expression mScarb2 in conferred partial susceptibility. Very few bound mScarb2-expressing cells. titer expressing was approximately...
Cytoplasmic and endosomal RNA sensors recognize virus infection signals to protect host cells by inducing type I IFN. The cytoplasmic sensors, retinoic acid inducible gene I/melanoma differentiation-associated 5, actually play pivotal roles in sensing replication. IFN-β promoter stimulator-1 (IPS-1) is their common adaptor for IFN-inducing signaling. Toll/IL-1R homology domain-containing molecule 1 (TICAM-1), also known as TRIF, the TLR3 that recognizes viral dsRNA early endosome dendritic...
Enterovirus 71 (EV71) is one of the major causative agents hand, foot and mouth disease (HFMD). Occasionally, EV71 infection associated with severe neurological diseases, such as acute encephalitis, flaccid paralysis cardiopulmonary failure. Several molecules act cell surface receptors that stimulate infection, including scavenger receptor B2 (SCARB2), P-selectin glycoprotein ligand-1 (PSGL-1), sialylated glycan, heparan sulfate annexin II (Anx2). SCARB2 plays critical roles in attachment,...
mRNAs for acetylcholine receptor genes are highly concentrated in the endplate region of adult skeletal muscle largely as a result transcription restricted to subneural nuclei. To identify regulatory elements involved, we employed DNA injection plasmid containing fragment delta-subunit gene promoter (positions -839 +45) linked reporter lacZ with nuclear localization signal. Injection wild-type construct into mouse leg muscles yielded preferential expression synaptic region. Analysis various...
Recombinant viruses between the virulent Mahoney and attenuated Sabin 1 strains of poliovirus type were subjected to neurovirulence tests using a transgenic (Tg) mouse line, ICR-PVRTg1, that carried human receptor gene. The Tg mice inoculated intracerebrally with these recombinant observed for clinical signs, histopathological lesions, viral antigens as parameters viruses. These in different Dose-dependent incidences paralysis death any used. This indicates values 50% lethal dose are useful...
Enterovirus 71 (EV71) is a causative agent of hand, foot, and mouth disease sometimes causes severe or fatal neurological complications. The amino acid at VP1-145 determines the virological characteristics EV71. Viruses with glutamic (E) (VP1-145E) are virulent in neonatal mice transgenic expressing human scavenger receptor B2, whereas those glutamine (Q) glycine (G) not. However, contribution this variation to pathogenesis humans not fully understood. We compared virulence VP1-145E VP1-145G...
Attachment receptors expressed on the host cell surface are thought to enhance EV71 infection by increasing chance of encountering true receptors. Although this has been confirmed using culture for some viruses, importance attachment in vivo is unknown. This report provides an unexpected answer question. We demonstrated that VP1-145G virus binds HS and shows attenuated phenotype hSCARB2-dependent animal model. highly cells express hSCARB2 at low or undetectable levels. Our data indicate...
<title>Abstract</title> Saffold virus (SAFV), a member of the species <italic>Cardiovirus saffoldi</italic> within <italic>Picornaviridae</italic> family, causes acute respiratory and gastrointestinal illnesses, as well hand, foot, mouth diseases. It is also suspected to be associated with neuronal disorders such encephalitis meningitis in severe cases. Despite its clinical significance, virus-host interactions underlying SAFV pathogenicity remain largely unknown. Using genome-wide...