A5102710509- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Retinal Imaging and Analysis
- Optical Coherence Tomography Applications
- Ocular Diseases and Behçet’s Syndrome
- Retinal and Optic Conditions
- Cytomegalovirus and herpesvirus research
- Retinal and Macular Surgery
- Intraocular Surgery and Lenses
- Corneal surgery and disorders
- Herpesvirus Infections and Treatments
- Influenza Virus Research Studies
- Ocular Surface and Contact Lens
- Ocular Infections and Treatments
- Viral Infections and Immunology Research
- Corneal Surgery and Treatments
- Retinal Development and Disorders
- Viral gastroenteritis research and epidemiology
- Traumatic Ocular and Foreign Body Injuries
- Lung Cancer Treatments and Mutations
- Immune Response and Inflammation
- Parvovirus B19 Infection Studies
- Medical Imaging and Pathology Studies
- Pneumonia and Respiratory Infections
- Lung Cancer Research Studies
Miyata Eye Hospital
2017-2025
Shinshu University
2023-2025
Tokyo Medical and Dental University
2022-2024
Tokyo Medical University
2009-2023
Mukogawa Women's University
2022-2023
Tokyo Medical University Ibaraki Medical Center
2011-2022
Showa University Fujigaoka Hospital
2011-2022
National Institute for Japanese Language and Linguistics
2022
Kitasato University
1991-2020
Kagoshima University
2015-2020
The role of secretory IgA in conferring cross-protective immunity was examined polymeric (p)IgR knockout (KO) mice immunized intranasally with different inactivated vaccines prepared from A/PR/8/34 (H1N1), A/Yamagata/120/86 A/Beijing/262/95 and B/Ibaraki/2/85 viruses infected the virus upper respiratory tract (RT)-restricting volume. In wild-type mice, immunization or its variant (A/Yamagata/120/86 A/Beijing/262/95) conferred complete protection partial cross-protection against infection,...
In response to stress, cells induce ribonucleoprotein aggregates, termed stress granules (SGs). SGs are transient loci containing translation-stalled mRNA, which is eventually degraded or recycled for translation. Infection of some viruses, including influenza A virus with a deletion nonstructural protein 1 (IAVΔNS1), induces SG-like aggregates. Previously, we showed that IAVΔNS1-induced required efficient induction type I interferon (IFN). Here, investigated SG formation by different...
Hepatitis C virus (HCV) core protein is a putative nucleocapsid with number of regulatory functions. In tissue culture cells, HCV mainly located at the endoplasmic reticulum as well mitochondria and lipid droplets within cytoplasm. However, it also detected in nucleus some cells. To elucidate mechanisms by which cellular trafficking controlled, we performed subcellular fractionation experiments used confocal microscopy to examine distribution heterologously expressed fusion proteins...
purpose. To evaluate the clinical significance of newly developed long-wavelength probe optical coherence tomography (LP-OCT) for diagnosis exudative macular diseases. methods. Fourteen eyes 13 participants were prospectively enrolled in study. There seven type I and five II choroidal neovascularization (CNV) cases associated with age-related degeneration idiopathic one case polypoidal vasculopathy (PCV). A custom-built LP-OCT based on swept-source OCT (SS-OCT) technology was used. This new...
The purpose of the present study was to evaluate intraretinal migration retinal pigment epithelium (RPE) cells in age-related macular degeneration (AMD) using polarimetry. We evaluated 155 eyes at various AMD stages. Depolarized light images were computed a polarization-sensitive scanning laser ophthalmoscope (PS-SLO), and degree polarization uniformity calculated optical coherence tomography (OCT). Each polarimetry image compared with corresponding autofluorescence (AF) 488 nm (SW-AF) 787...
Among the enteroviruses, polioviruses and enterovirus 71 (EV71) are two major neurotropic viruses causing serious neurological manifestations. While being eradicated globally by vaccination, EV71 still has potential to cause a large outbreak such as that in Taiwan 1998, which there were many fatalities. In this study, we determined neurovirulence of neuropathological analysis cynomolgus monkeys after experimental infection with five strains, isolated from individual patients fatal...
Poliovirus and enterovirus 71 (EV71) are both neurotropic enteroviruses that cause serious neurological diseases, such as poliomyelitis encephalitis. The neurovirulence of EV71 in cynomolgus monkeys was demonstrated previously by intraspinal inoculation. In this study, an improved simian model infection established using intravenous inoculation, which revealed clinical neuropathological similarities between human cases Experimental induced direct manifestations, tremor, ataxia brain oedema,...
The organization of multisynaptic projections from the prefrontal cortex to primary motor (MI) was examined in macaque monkeys by retrograde transneuronal transport rabies virus. In first series experiments, virus injected into MI forelimb region, and time-dependent distribution patterns transsynaptic labeling were analyzed frontal lobe with various survivals (2-4 d). Two days after viral injection, neuronal emerged caudal aspects nonprimary motor-related areas that are known project...
Examination of nucleotide diversity in 106 mitochondrial genomes the most geographically widespread human malaria parasite, Plasmodium vivax, revealed a level similar to, but slightly higher than, that seen virulent parasite falciparum. The pairwise distribution differences among genome sequences supported hypothesis both these parasites underwent ancient population expansions. We estimated age recent common ancestor (MRCA) P. vivax and falciparum at around 200,000-300,000 years ago. This is...
purpose. To evaluate the birefringence properties of eyes with age-related macular degeneration (AMD). compare information from two techniques—scanning laser polarimetry (GDx) and polarization-sensitive spectral-domain optical coherence tomography (OCT)—and investigate how they complement each other. methods. The authors prospectively examined healthy subjects 13 patients exudative AMD. Using scanning polarimetry, computed phase-retardation maps, average reflectance images, depolarized light...
The purpose of this study was to simultaneously measure the concentrations multiple cytokines, including vascular endothelial growth factor, in vitreous patients with diabetic retinopathy and examine their relation clinical findings.Vitreous samples from 46 eyes 19 nondiabetic macular disease (controls) were used. Nine cytokines measured using a FACSCalibur flow cytometer.Vascular interleukin-8, monocyte chemotactic protein-1, interferon-inducible protein-10, monokine induced by...
Epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitor (TKI) therapy often provides a dramatic response in lung cancer patients with EGFR mutations. In addition, moderate clinical efficacy of the EGFR-TKI, erlotinib, has been shown wild-type EGFR. Numerous molecular mechanisms that cause acquired resistance to EGFR-TKIs have identified cancers mutations; however, few reported We used H358 adenocarcinoma cells lacking mutations showed modest sensitivity erlotinib. The erlotinib via...
ABSTRACT To establish a sensitive and specific antibody assay, potent antigenic proteins encoded by human herpesvirus 8 (HHV8) were studied. Fifteen recombinant HHV8-encoded produced as glutathione S -transferase fusion proteins. The sera from AIDS-associated Kaposi's sarcoma (KS) patients reacted with four open reading frames (ORFs) K8.1, 59, 65, 73 in Western blot assay. An enzyme-linked immunosorbent assay (ELISA) using these antigens (mixed-antigen ELISA) revealed that all 26 derived KS...
Mice (ddY strain, 4 weeks old) were infected intranasally with the H5N1 influenza viruses A/Hong Kong/156/97 (HK156) and Kong/483/97 (HK483) isolated from humans. HK156 HK483 required 200 5 p.f.u. of virus, respectively, to give a 50% lethal dose mice when volume inoculum was set at 10 μl. Both caused encephalitis severe bronchopneumonia in mice. The severity lung lesions by essentially similar, whereas more extensive brain than did HK156. This supported results virus titration organ...
BACKGROUND Bowen's disease (BD) of the genital skin region is generally associated with human papillomavirus (HPV) infection. Various molecular analyses have identified mainly HPV-16 in lesions. However, HPV genotypes BD hands not yet been characterized. METHODS The specimens 12 patients were investigated clinicopathologically and immunohistochemically, total DNAs extracted from analyzed for presence DNA using Southern blot hybridization polymerase chain reaction (PCR) combination...
In order to study the pathogenesis of HHV-6 infection in central nervous system disorders, U251 cell line was infected with freshly isolated variant B HHV-6. Although IEA/ex 3 antigen (immediate early protein) detected nuclei, neither presence OHV-3 (late antigen) nor production infectious virus demonstrated. These results indicate that abortive established cells. After viral infection, genome copy cells gradually decreased and became stable after 6 passages. At point, gene expression...
To demonstrate the clinical application of a noninvasive, three-dimensional, vascular imaging technique called Doppler optical coherence angiography (OCA). evaluate architecture polypoidal choroidal vasculopathy (PCV) using OCA.The authors prospectively examined eyes four healthy subjects and 15 PCV patients. Three-dimensional flow was performed high-speed, high-resolution, high-penetration spectral-domain tomography. Two-dimensional images retina, choroid, lesions were obtained...