- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Genetics and Plant Breeding
- Genetic Mapping and Diversity in Plants and Animals
- Bioenergy crop production and management
- Plant Pathogens and Resistance
- Chromosomal and Genetic Variations
- Yeasts and Rust Fungi Studies
- Crop Yield and Soil Fertility
- Agriculture, Plant Science, Crop Management
- Phytase and its Applications
- Berry genetics and cultivation research
- Horticultural and Viticultural Research
- Food composition and properties
- Photosynthetic Processes and Mechanisms
- Plant Genetic and Mutation Studies
- Turfgrass Adaptation and Management
- Microtubule and mitosis dynamics
- Plant Reproductive Biology
- Soybean genetics and cultivation
- Mycotoxins in Agriculture and Food
- Agricultural Productivity and Crop Improvement
- Plant tissue culture and regeneration
Edward T. Schafer Agricultural Research Center
2013-2025
Agricultural Research Service
2002-2021
United States Department of Agriculture
1982-2021
North Dakota State University
1992-2007
Montana State University
1980
Abstract Chromosome engineering is a useful strategy for transfer of alien genes from wild relatives into modern crops. However, this has not been extensively used gene introgression in most crops due to low efficiency conventional cytogenetic techniques. Here, we report an improved scheme chromosome efficient elimination large amount goatgrass (Aegilops speltoides) chromatin surrounding Sr39, that provides resistance multiple stem rust races, including Ug99 (TTKSK) wheat. The wheat ph1b...
Abstract The transfer of alien genes to crop plants using chromosome engineering has been attempted infrequently in tetraploid durum wheat (Triticum turgidum L. subsp. durum). Here, we report a highly efficient approach for the two conferring resistance stem rust race Pgt-TTKSK (Ug99) from goatgrass (Aegilops speltoides) wheat. line DAS15, carrying gene Sr47 derived Ae. speltoides, was crossed, and backcrossed, 5D(5B) aneuploids induce homeologous pairing. After final cross ‘Rusty’ durum,...
Abstract The wheat ( Triticum aestivum L.) cultivar Waldron carries at least six genes for resistance to stem rust caused by Puccinia graminis Pers.:Pers. f.sp. tritici Eriks. and Henn. Pgt ). identity map location of some these are known, but there is limited knowledge SrWld1 , an important gene that conditions all North American races. Our objective was identify additional in through aneuploid analysis, allelism tests, linkage with molecular markers. Four lines, WDR‐A1, WDR‐C2, WDR‐D1,...
ABSTRACT Stem rust (caused by Puccinia graminis Pers.:Pers. f. sp. tritici Eriks. & E. Henn.), a devastating disease of wheat ( Triticum aestivum L.), was effectively controlled worldwide for the past 50 yr deployment stem resistance Sr ) genes in cultivars. However, new race, TTKSK (known as Ug99 or TTKS) that emerged eastern Africa, is cause concern because it has broad virulence to currently deployed genes. To identify potentially sources effective against Ug99, we evaluated and...
ABSTRACT In this study we assess the genetic architecture of bread‐making quality traits in spring wheat ( Triticum aestivum L.). A mapping population derived from BR34 and ‘Grandin’ was used to measure 20 end‐use including six kernel, seven milling flour, four dough mixing strength, three traits. total 31 quantitative trait loci (QTL) significantly associated with all but two were identified. These QTL clustered five chromosomal regions, namely 1BS, 1DL, 4BL, 5BL, 6AS, explained a large...
A genetic suppressor of resistance to stem rust, Puccinia graminis Pers.:Pers. f. sp. tritici Eriks. & E. Henn., wheat ( Triticum aestivum L.) is on the long arm chromosome 7D. Mutation or inactivation gene would allow expression suppressed genes already present in hexaploid transferred from diploid tetraploid relatives. Our objective was induce a mutation and describe its inheritance. Seed treatment cultivar Canthatch (CTH) with ethyl methanesulfonate produced 15 rust‐resistant mutants....
The genetics of resistance to stem rust (caused by Puccinia graminis Pers.:Pers. f. sp. tritici Eriks. and Henn.) in durum ( Triticum turgidum L. ssp. ) is not as well understood for bread wheat T. aestivum L.). Our objective was determine the chromosomal location genes four monogenic lines derived from Ethiopian tetraploid landrace ST464. were crossed a set susceptible aneuploids based on line 47‐1. We observed chromosome pairing hybrids made testcrosses ‘Rusty’ durum. Monogenic ST464‐A1...
Abstract Wheat stem rust, caused by Puccinia graminis f. sp. tritici Eriks. & E. Henn, can incur yield losses in susceptible cultivars of durum wheat, Triticum turgidum ssp. (Desf.) Husnot. Although several possess the rust resistance gene Sr13, additional genes wheat effective against emerging virulent races have not been described. Durum line 8155-B1 confers P. race TTKST, variant Ug99 group with virulence to Sr24. However, does confer first-described group: TTKSK. We mapped a single...
The USDA–ARS National Small Grains Collection (NSGC) maintains germplasm representing global diversity of small grains and their wild relatives. To evaluate the utility NSGC durum wheat ( Triticum turgidum L. ssp. ) accessions, we assessed genetic linkage disequilibrium (LD) patterns in a core subset containing 429 lines with spring growth habit originating from 64 countries worldwide. Genetic estimated using single‐nucleotide polymorphism (SNP) markers showed considerable captured this...
ABSTRACT High molecular weight (HMW) glutenin subunits (GSs) play an important role in determining end‐use quality wheat ( Triticum aestivum L.). They are also excellent protein markers for genotype identification. In this study, we analyzed the HMW‐GSs Thinopyrum bessarabicum (Savul. and Rayss) Á. Löve, Th. intermedium (Host) Barkworth D.R. Dewey, Lophopyrum elongatum A. markgrafii (Greuter) Hammer genotyped eight sets of 44 disomic addition lines derived from these species common or durum...
ABSTRACT Yield reduction due to linkage drag is a major problem when introgressing alien chromatin into durum wheat [ Triticum turgidum L. subsp. (Desf.) Husn.]. Here we report attempts compensate for yield losses associated with in 1AS·1AL‐1DL translocation lines of wheat. Near‐isogenic (NILs) were produced by six backcrosses stock cultivars Renville, Rugby, Lebsock, Ben, and Maier. Rugby was found be composed two biotypes, one biotype having high molecular weight (HMW) glutenin subunits...
Few genetic male sterility (GMS) genes have been mapped in hexaploid wheat (Triticum aestivum L.). Our objective was to locate chromosomes the GMS mutants FS2, FS3, FS20, and FS24. We crossed each mutant Cornerstone sterile, which has ms1c allele, determine allelic relationships. FS20 'Chris' monosomics, observed segregation F2 backcrosses FS20. After observing results, we made appropriate crosses Chinese Spring (CS) ditelosomic lines map chromosome arms. The allelism test indicated that...
ABSTRACT Race TTKSK (Ug99) of Puccinia graminis f. sp. tritici is a serious threat to wheat production worldwide because its wide virulence on many cultivars and rapid spread. Emmer [ Triticum turgidum L. subsp. dicoccon (Schrank) Thell.] known be source resistance stem rust but has not been evaluated against race TTKSK. In attempts identify characterize genes effective at the seedling stage, we 359 accessions emmer with other races broad virulence. A high frequency (31.8%) were resistant...
The supernumerary spikelet (SS) trait of durum wheat (Triticum turgidum L.), including the ramified and four-rowed spike traits, is characterized by an increased number spikelets per spike. Chromosomal location SS gene(s) was determined crossing line PI349056 to set 'Langdon' D-genome disomic substitution lines. Double monosomic F 1 plants were backcrossed testcross classified for chromosome pairing type. Segregation type observed in 2 . Data indicated that major gene located on 2A....
The aneuploid stocks of durum wheat (Triticum turgidum L. subsp. (Desf.) Husnot) and common (T. aestivum L.) have been developed mainly in 'Langdon' (LDN) 'Chinese Spring' (CS) cultivars, respectively. LDN-CS D-genome chromosome disomic substitution (LDN-DS) lines, where a pair CS chromosomes substitute for corresponding homoeologous A- or B-genome LDN, widely used to determine the chromosomal locations genes tetraploid wheat. LDN-DS lines were originally by crossing nulli-tetrasomics with...
ABSTRACT In the tribe Triticeae, several Thinopyrum species have been used as sources of resistance to stem rust (caused by Puccinia graminis Pers.:Pers. f. sp. tritici Eriks. & E. Henn., abbreviated Pgt ) and other wheat ( Triticum aestivum L.) diseases. To identify novel race TTKSK (Ug99), we evaluated characterized 242 accessions belonging five species, including beach wheatgrass [ Th. bessarabicum (Savul. Rayss) A. Löve], diploid tall elongatum (Host) D.R. Dewey], intermediate...