A5036184011- Photodynamic Therapy Research Studies
- Nanoplatforms for cancer theranostics
- Porphyrin and Phthalocyanine Chemistry
- bioluminescence and chemiluminescence research
- Advanced Fluorescence Microscopy Techniques
- Analytical Chemistry and Sensors
- Luminescence and Fluorescent Materials
- Fermentation and Sensory Analysis
- Dendrimers and Hyperbranched Polymers
- Photosynthetic Processes and Mechanisms
- Photoreceptor and optogenetics research
- Molecular Sensors and Ion Detection
- Horticultural and Viticultural Research
- Corneal Surgery and Treatments
- Light effects on plants
- Synthesis and Biological Evaluation
- Click Chemistry and Applications
- Cancer therapeutics and mechanisms
- Phytochemicals and Antioxidant Activities
- Computational Drug Discovery Methods
- Metal complexes synthesis and properties
- Antimicrobial Peptides and Activities
- Retinal Development and Disorders
- Cancer Research and Treatments
- Advanced Biosensing Techniques and Applications
Institut Químic de Sarrià
2011-2022
Universitat Ramon Llull
2011-2022
University of California, San Francisco
2016-2017
FC Barcelona
2016-2017
The genetically encodable fluorescent tag miniSOG is expected to revolutionize correlative light- and electron microscopy due its ability produce singlet oxygen upon light irradiation. quantum yield of this process was reported as ΦΔ = 0.47 ± 0.05, derived from miniSOG's photooxidize the probe anthracene dipropionic acid (ADPA). In report, a significantly smaller value 0.03 0.01 obtained by two methods: direct measurement phosphorescence at 1275 nm chemical trapping using uric an alternative...
Structures of typical photosensitizers used in antimicrobial photodynamic therapy are based on porphyrins, phthalocyanines, and phenothiazinium salts, with cationic charges at physiological pH values. However, derivatives the porphycene macrocycle (a structural isomer porphyrin) have barely been investigated as agents. Therefore, we report synthesis first tricationic water-soluble its basic photochemical properties. We successfully tested it for vitro photoinactivation different...
Antimicrobial photodynamic therapy (aPDT) is an emerging treatment for bacterial infections that becoming increasingly more attractive because of its effectiveness against multi-antibiotic-resistant strains and unlikelihood inducing resistance. Among the strategies to enhance efficacy PDT Gram-negative bacteria, binding a cationic antimicrobial peptide offers prospect improving both water solubilty localization photoactive drug in bacteria. In this work we have compared number free...
Abstract A biocompatible fluorescent nanoprobe for singlet oxygen ( 1 O 2 ) detection in biological systems was designed, synthesized, and characterized, that circumvents many of the limitations molecular probe Singlet Oxygen Sensor Green ® (SOSG). This widely used commercial covalently linked to a polyacrylamide nanoparticle core using different architectures optimize response . In contrast its counterpart, optimum SOSG‐based nanoprobe, which we call NanoSOSG, is readily internalized by E....
Over the last 20 years, number of pathogenic multi-resistant microorganisms has grown steadily, which stimulated search for new strategies to combat antimicrobial resistance. Antimicrobial photodynamic therapy (aPDT), also called inactivation, is emerging as a promising alternative treatments based on conventional antibiotics. We have explored effectiveness methylene blue-loaded targeted mesoporous silica nanoparticles (MSNP) in inactivation two Gram negative bacteria, namely Escherichia...
Cationic antimicrobial peptides (CAMPs) and photodynamic therapy (PDT) are attractive tools to combat infectious diseases stem further development of antibiotic resistance. In an attempt increase the efficiency bacteria inactivation, we conjugated a PDT photosensitizer, cationic or neutral porphyrin, CAMP, buforin magainin. The hydrophobic which is not photoactive per se against Gram-negative bacteria, efficiently photoinactivated Escherichia coli after conjugation either Conjugation...
A combination of therapies to treat cancer malignancies is at the forefront research with aim reduce drug doses (ultimately side effects) and diminish possibility resistance emergence given multitarget strategy. With this goal in mind, present study, we report between chemotherapeutic doxorubicin (DOXO) photosensitizing agent pheophorbide a (PhA) inactivate HeLa cells. Photophysical studies revealed that DOXO can quench excited states PhA, detracting from its ability. itself photosensitize...
Flavin-binding fluorescent proteins (FPs) are genetically encoded in vivo reporters, which derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed ROS formation light-driven antimicrobial efficacy of eleven LOV-based FPs. particular, determined singlet oxygen (1O2) quantum yields superoxide photosensitization activities via spectroscopic assays performed cell toxicity experiments E. coli. Besides miniSOG SOPP, have been engineered to generate 1O2, all...
Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants their ability to photo-oxidize diaminobenzidine, both key aspects CLEM. Our experiments reveal a complex relation between these parameters production different reactive oxygen species.
Abstract miniSOG, developed as the first fully genetically encoded singlet oxygen photosensitiser, has found various applications in cell imaging and functional studies. Yet, miniSOG suboptimal properties, including a low yield of generation, which can nevertheless be improved tenfold upon blue light irradiation. In previous study, we showed that this improvement was due to photolysis chromophore, flavin mononucleotide (FMN), into lumichrome, with concomitant removal phosphoribityl tail,...
In this study, we report the synthesis and photochemical behavior of a new family photoactive compounds to assess its potential as singlet oxygen ((1)O2) probes. The candidate dyads are composed by (1)O2 trap plus naphthoxazole moiety linked directly or through an unsaturated bond oxazole ring. native state, inherent great fluorescence is quenched; but in presence (1)O2, generated addition appropriate irradiation external photosensitizer, photooxidation reaction occurs leading formation...
Fluorescent proteins (FPs) are powerful tools for cell and molecular biology. Here based on structural analysis, a blue-shifted mutant of recently engineered monomeric infrared fluorescent protein (mIFP) has been rationally designed. This variant, named iBlueberry, bears single mutation that shifts both excitation emission spectra by approximately 40 nm. Furthermore, iBlueberry is four times more photostable than mIFP, rendering it advantageous imaging dynamics. By tagging to centrin,...
Synthesis, photophysical properties and photoantimicrobial efficiency of cationic Zn(<sc>ii</sc>) Ru(<sc>ii</sc>) dendrimeric phthalocyanines.
Over the last decades, number of pathogenic multi-resistant microorganisms has grown dramatically, which stimulated search for novel strategies to combat antimicrobial resistance. Antimicrobial photodynamic therapy (aPDT) is one promising alternatives conventional treatments based on antibiotics. Here, we present a comparative study two aryl tricationic porphycenes where photoinactivation efficiency against model correlated photophysical behavior porphycene derivatives. Moreover, extent...
Inflammation and shear stress can upregulate expression of cellular adhesion molecules in endothelial cells (EC). The modified EC surface becomes a mediating interface between the circulating blood elements endothelium, grants opportunity for immunotherapy. In photodynamic therapy (PDT), immunotargeting might overcome lack selectivity currently used sensitizers. this study, we hypothesized that differential ICAM-1 modulates effects drug targeted to ICAM-1. A novel porphycene-anti-ICAM-1...