A5091817261- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Cancer-related Molecular Pathways
- Multiple Myeloma Research and Treatments
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Cancer Research and Treatments
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- CAR-T cell therapy research
- interferon and immune responses
- HIV/AIDS drug development and treatment
- Heat shock proteins research
- Cancer therapeutics and mechanisms
- Telomeres, Telomerase, and Senescence
- Cancer Immunotherapy and Biomarkers
- Genomics, phytochemicals, and oxidative stress
- Toxin Mechanisms and Immunotoxins
- Immune Cell Function and Interaction
- Tea Polyphenols and Effects
- Viral Infectious Diseases and Gene Expression in Insects
- Ovarian cancer diagnosis and treatment
- Cancer-related molecular mechanisms research
- Research on Leishmaniasis Studies
Wayne State University
2012-2021
John D. Dingell VA Medical Center
2011-2020
The Barbara Ann Karmanos Cancer Institute
2008-2013
Harvard University
2005-2008
VA Boston Healthcare System
2004-2008
Dana-Farber Cancer Institute
2005-2007
University of Arkansas for Medical Sciences
1994-2005
University of Basel
2005
Oklahoma State University
2005
Rush University Medical Center
2005
In cancer cells, telomerase induction helps maintain telomere length and thereby bypasses senescence provides enhanced replicative potential. Chemical inhibitors of have been shown to reactivate shortening cause apoptotic cell death tumor cells while having little or no effect on normal diploid cells.We designed siRNAs against two different regions gene evaluated their length, proliferative potential, expression in Barrett's adenocarcinoma SEG-1 cells. The mixture nanomolar concentrations...
Ovarian cancer is the leading cause of mortality from gynecological malignancies, often undetectable in early stages. The difficulty detecting disease its stages and propensity ovarian cells to develop resistance known chemotherapeutic treatments dramatically decreases 5-year survival rate. Chemotherapy with paclitaxel after surgery increases median only by 2 3 years stage IV highlights need for more effective drugs. human immunodeficiency virus (HIV) infection characterized increased risk...
The incidence of Barrett esophageal adenocarcinoma (BEAC) has been increasing at an alarming rate in western countries. In this study, we have evaluated the therapeutic potential sulforaphane (SFN), antioxidant derived from broccoli, BEAC.BEAC cells were treated with SFN, alone or combination chemotherapeutic, paclitaxel, telomerase-inhibiting agents (MST-312, GRN163L), and live cell number determined various time points. effect on drug resistance/chemosensitivity was by rhodamine efflux...
Abstract Background Sulforaphane (SFN), an isothiocyanate phytochemical present predominantly in cruciferous vegetables such as brussels sprout and broccoli, is considered a promising chemo-preventive agent against cancer. In-vitro exposure to SFN appears result the induction of apoptosis cell-cycle arrest variety tumor types. However, molecular mechanisms leading inhibition cell cycle progression by are poorly understood epithelial ovarian cancer cells (EOC). The aim this study understand...
Recent observations suggest a lower incidence of malignancies in patients infected with HIV during treatment Highly Active Anti-Retroviral Therapy (HAART) utilizing protease inhibitors. We investigated the effects ritonavir, FDA approved inhibitor, on proliferation pancreatic ductal adeno-carcinoma (PDAC) cell lines. Human PDAC lines BxPC-3, MIA PaCa-2, and PANC-1 were propagated under standard conditions treated serial dilutions ritonavir. Ritonavir inhibited growth dose-dependent manner as...
Abstract Myeloma vaccines, based on dendritic cells pulsed with idiotype or tumor lysate, have been met limited success, probably in part due to insufficient cross-priming of myeloma antigens. A powerful method introduce myeloma-associated antigens into the cytosol is protein transduction, a process by which proteins fused transduction domain (PTD) freely traverse membrane barriers. NY-ESO-1, an immunogenic antigen itself highly expressed 60% high-risk patients, was purified near homogeneity...
The 90-kDa heat shock protein (Hsp90) plays an important role in conformational regulation of cellular proteins and thereby signaling function. As Hsp90 is considered a key component immune function its inhibition has become target for cancer therapy, we here evaluated the human dendritic cell (DC) phenotype significantly decreased surface expression costimulatory (CD40, CD80, CD86), maturation (CD83), MHC (HLA-A, B, C HLA-DP, DQ, DR) markers immature DC mature was associated with...
Abstract Purpose: The aims of this study were to investigate telomere function in normal and Barrett's esophageal adenocarcinoma (BEAC) cells purified by laser capture microdissection evaluate the effect telomerase inhibition cancer vitro vivo. Experimental Design: Epithelial from surgically resected esophagi. Telomerase activity was measured modified telomeric repeat amplification protocol length determined real-time PCR assay. To inhibition, cell lines continuously treated with a specific...
Objective: To compare the survival of patients with bilateral versus unilateral malignant ovarian germ cell tumors (OGCT). Methods: Patients a diagnosis OGCT were identified from Surveillance, Epidemiology, and End Results Program for period 1988 to 2006 divided into subgroups. Only surgically treated included. Histologic types grouped dysgerminoma, teratoma, mixed pure nondysgerminoma tumors. Statistical analysis using Wilcoxon rank sum test, Kaplan-Meier methods, Cox proportional hazards...