A5046068796- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Glycosylation and Glycoproteins Research
- Neurological Disease Mechanisms and Treatments
- Genetic Neurodegenerative Diseases
- Ion Channels and Receptors
- Immune cells in cancer
- Neuroscience and Neuropharmacology Research
- Immune Response and Inflammation
- Barrier Structure and Function Studies
- Advanced Glycation End Products research
- Mitochondrial Function and Pathology
- Cancer-related Molecular Pathways
- DNA Repair Mechanisms
- Gold and Silver Nanoparticles Synthesis and Applications
- Inflammation biomarkers and pathways
- Lysosomal Storage Disorders Research
- Cholesterol and Lipid Metabolism
- T-cell and B-cell Immunology
- Neurological diseases and metabolism
- Histone Deacetylase Inhibitors Research
- Glycogen Storage Diseases and Myoclonus
- Sodium Intake and Health
- Cerebrovascular and genetic disorders
Universitat Autònoma de Barcelona
2018-2024
Universitat de Barcelona
2009-2022
Biomedical Research Networking Center on Neurodegenerative Diseases
2010
Late-onset Alzheimer's disease (AD) is the most common form of AD appearing after 65 years age. To date, however, there are no non-genetically manipulated rodent models that develop a similar sporadic onset with age-related amyloid-beta (Abeta) deposition. Although senescence accelerated mouse prone 8 (SAMP8) mice have been proposed as model AD, presence Abeta deposits remains controversial. In this study, we describe time course deposition in SAMP8 well control SAMR1 and ICR-CD1 strains...
Receptor-mediated transcytosis has been widely studied as a possible strategy to transport neurotherapeutics across the blood−brain barrier (BBB). Monoclonal antibodies directed against transferrin receptor (TfR) have proposed potential carrier candidates. A better understanding of mechanisms involved in their cellular uptake and intracellular trafficking is required could critically contribute improvement delivery methods. Accordingly, we here gold nanoparticles (AuNPs) coated with 8D3...
Lafora disease (LD), the most devastating adolescence-onset epilepsy, is caused by mutations in EPM2A or EPM2B genes, which encode proteins laforin and malin, respectively. Loss of function one these proteins, are involved regulation glycogen synthesis, induces accumulation polyglucosan bodies (PGBs)-known as (LBs) associated with neurons-in brain. Ageing some neurodegenerative conditions lead to appearance another type PGB called corpora amylacea, astrocytes contain neo-epitopes that can be...
Hypertension is the most prevalent modifiable risk factor for stroke and associated with worse functional outcomes. Pharmacological inhibition of histone deacetylases by suberoylanilide hydroxamic acid (SAHA) modulates gene expression has emerged as a promising therapeutic approach to reduce ischaemic brain injury. Here, we have tested potential SAHA administered during reperfusion in adult male spontaneously hypertensive (SHR) rats subjected transient middle cerebral artery occlusion...
The senescence accelerated mouse-prone 8 (SAMP8) strain of mice is an experimental model that has also been proposed as a Alzheimer's disease it shares several features with this dementia. We have recently report
J. Duran‐Vilaregut, del Valle, G. Manich, A. Camins, M. Pallàs, Vilaplana and C. Pelegrí (2011) Neuropathology Applied Neurobiology 37, 525–537 Role of matrix metalloproteinase (MMP)‐9 in striatal blood–brain barrier disruption a 3‐nitropropionic acid model Huntington's disease Aims: 3‐Nitropropionic (3‐NPA) is natural toxin that, when administered to experimental animals, reproduces the brain lesions observed disease, which mainly consist selective neurodegeneration striatum. The also...
Cerebrovascular dysfunction and β-amyloid peptide deposition on the walls of cerebral blood vessels might be an early event in development Alzheimer’s disease. Here we studied time course amyloid blood-brain barrier (BBB) disruption CA1 subzone hippocampus SAMP8 mice association between these two variables. We also recently described clusters parenchyma, as well with which BBB is disrupted. showed greater than age-matched ICR-CD1 control mice. Moreover, at 12 months age number a disrupted...
Abstract Senescence of the cerebrovascular system and an abnormal function blood–brain barrier have been related with Alzheimer's disease. We studied here time‐course disruption in senescence‐accelerated mouse prone 8 (SAMP8) mice, which is a murine model senescence also considered used previously described method that allows evaluating integrity by observing Evans blue extravasation from brain blood vessels. Three regions (cortex, hippocampus hippocampal fissure) SAMP8 brains were analyzed...
Transient potential receptor vanilloid 2 (TRPV2) is widely expressed through the nervous system and specifically found in neuronal subpopulations some glial cells. TRPV2 known to be sensitized by methionine oxidation, which results from inflammation. Here we aim characterize expression regulation of myelination pathologies, such as hypomyelination demyelination. We validated interaction between its putative interactor Opalin, an oligodendrocyte marker, mixed cultures under pro-...
Microglia are the main immune cells of central nervous system (CNS), and they devoted to active surveillance CNS during homeostasis disease. In last years, microglial receptor Triggering Receptor Expressed on Myeloid cells-2 (TREM2) has been defined mediate several functions, including phagocytosis, survival, proliferation, migration, be a key regulator new common signature induced under neurodegenerative conditions aging, also known as disease-associated microglia (DAM). Although TREM2...
Abstract Background Degenerative granular structures appear progressively with age in the hippocampus of most mouse strains. We recently reported that these granules contain a neo-epitope is recognised by IgM antibodies present as contaminants many commercial obtained from ascites and or rabbit serum. hypothesise anti-neo-epitope IgMs are fact natural auto-antibodies generated spontaneously during foetal stage without previous contact external antigens whose repertoire reactivity pattern...
Transient receptor potential vanilloid 2 (TRPV2) channels are expressed in both smooth muscle and endothelial cells participate vascular mechanotransduction sensing of high temperatures lipids. Nevertheless, the impact TRPV2 channel activation by agonists on coordinated cell-type specific modulation vasoreactivity is unknown.Aorta from 2- to 4-months-old male Oncins France 1 mice was dissected mounted tissue baths for isometric tension measurements. expression assessed immunofluorescence...
Demyelinating disorders show impaired remyelination due to failure in the differentiation of oligodendrocyte progenitor cells (OPCs) into mature myelin-forming oligodendrocytes, a process driven by microglia–OPC crosstalk. Through conducting transcriptomic analysis microarray studies on demyelination–remyelination cuprizone model and using human samples multiple sclerosis (MS), we identified molecules involved this Differentially expressed genes (DEGs) specific regions/cell types were...
Key events in postnatal brain development, such as neuronal migration, synaptogenesis, and myelination, shape the adult brain. These are reflected changes gray white matter (GM WM) occurring during this period. Therefore, precise knowledge of GM WM composition perinatal development is crucial to characterizing formation well neurodevelopmental disruption observed diseases autism schizophrenia. In study, we combined histochemical immunohistochemical staining with biochemical biophysical...
Abstract 3‐Nitropropionic acid (3‐NPA) is a mitochondrial toxin used in the laboratory to replicate neurodegenerative conditions that are accompanied by degeneration of caudate‐putamen. 3‐NPA induces depletion ATP production, reactive oxygen species and secondary excitotoxicity mediated activation N‐methyl‐D‐aspartate receptors culminates triggering cell death mechanisms, including apoptosis. We here examined immunohistochemical methods whether cellular expression phospho Ser1981 ‐ataxia...
Abstract Determination of microglial phagocytosis myelin has acquired importance in the study demyelinating diseases. One strategy to determine capacity consists assaying microglia with fluorescently labeled myelin; however, most approaches are performed cell culture, where usually show important phenotypic differences compared vivo conditions. In this article we describe an adapted flow cytometry protocol assay by obtained directly from tissue central nervous system. Key steps for a first...