A5029126828- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Autophagy in Disease and Therapy
- Genomics, phytochemicals, and oxidative stress
- Cancer, Hypoxia, and Metabolism
- PI3K/AKT/mTOR signaling in cancer
- Peroxisome Proliferator-Activated Receptors
- Nitric Oxide and Endothelin Effects
- Cell death mechanisms and regulation
- Liver Disease Diagnosis and Treatment
- Cancer Cells and Metastasis
- Circular RNAs in diseases
- Cancer Genomics and Diagnostics
- Redox biology and oxidative stress
- Cancer, Lipids, and Metabolism
- Extracellular vesicles in disease
- Microtubule and mitosis dynamics
- Mitochondrial Function and Pathology
- AI in cancer detection
- Drug-Induced Hepatotoxicity and Protection
- Heat shock proteins research
- Marine Toxins and Detection Methods
- RNA Research and Splicing
- Immune cells in cancer
- Inflammasome and immune disorders
Universidad de Sevilla
2019-2024
Centre de Recherche des Cordeliers
2024
Instituto de Biomedicina de Sevilla
2020-2024
Hospital Universitario Virgen del Rocío
2019-2024
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2023-2024
Centro de Investigación Biomédica en Red
2016-2022
Instituto de Salud Carlos III
2016-2022
Centro Andaluz de Biología del Desarrollo
2016-2017
Consejo Superior de Investigaciones Científicas
2016-2017
Universidad Pablo de Olavide
2016
Abstract The NLRP3 inflammasome has emerged as an important regulator of metabolic disorders and age-related diseases in NLRP3-deficient mice. In this article, we determine whether, old mice C57BL6J, the inhibitor MCC950 is able to attenuate syndrome providing health benefits. We report that attenuates hepatic dysfunction aged addition, inhibited Pi3K/AKT/mTOR pathway, enhanced autophagy, activated peroxisome proliferator-activated receptor-α vivo vitro. data suggest mediates protective...
Abstract Background and Aims Liver cancer stem cells (CSCs) could be involved in the carcinogenesis, recurrence, metastasis chemoresistance of hepatocellular carcinoma (HCC). The aim this study was to explore role lncRNA‐ H19 as a biomarker for liver cancer. Methods LncRNA‐ expression levels functional assays were conducted EpCAM + CD133 CSCs C57BL/6J mice fed with high‐fat high‐cholesterol carbohydrate (HFHCC) or standard diet 52 weeks. tissue plasma samples from patients cirrhosis, without...
Background: Sorafenib constitutes a suitable treatment alternative for patients with advanced hepatocellular carcinoma (HCC) in whom atezolizumab + bevacizumab therapy is contraindicated. The aim of the study was identification miRNA signature liquid biopsy related to sorafenib response. Methods: miRNAs were profiled hepatoblastoma HepG2 cells and tested animal models, extracellular vesicles (EVs), plasma from HCC patients. Results: altered expression 11 cells. miR-200c-3p miR-27a-3p exerted...
Circulating Tumor Cells (CTCs) are considered a prognostic marker in pancreatic cancer. In this study we present new approach for counting CTCs and CTC clusters patients with cancer using the IsofluxTM System Hough transform algorithm (Hough-IsofluxTM). The Hough-IsofluxTM is based on of an array pixels nucleus cytokeratin expression excluding CD45 signal. Total including free were evaluated healthy donor samples mixed cells (PCCs) from ductal adenocarcinoma (PDAC). manual was used blinded...
Hepatocellular carcinoma (HCC) represents 80% of the primary hepatic neoplasms. It is sixth most frequent neoplasm, fourth cause cancer-related death, and 7% registered malignancies. Sorafenib first line molecular targeted therapy for patients in advanced stage HCC. The present study shows that exerts free radical scavenging properties associated with downregulation nuclear factor E2-related 2 (Nrf2)-regulated thioredoxin 1 (Trx1) expression liver cancer cells. experimental and/or...
Macrophages constitute a major part of the tumor-infiltrating immune cells. Within tumor microenvironment, they acquire an alternatively activated, tumor-supporting phenotype. Factors released by cells are crucial for recruitment tumor-associated macrophages. In present project, we aimed to understand role hsa-miR-200c-3p (miR-200c) in interplay between and To this end, employed coculture system MCF7 breast primary human macrophages observed transfer miR-200c from apoptotic macrophages,...
Autophagy is a highly conserved degradation mechanism that essential for maintaining cellular homeostasis. In human disease, autophagy pathways are frequently deregulated and there immense interest in targeting therapeutic approaches. Accordingly, need to determine autophagic activity tissues, an endeavor hampered by the fact characterized flux of substrates whereas histology informs only about amounts localization regulators at single timepoint. Despite this challenging task, considerable...
Okadaic acid (OA) has been an invaluable pharmacological tool in the study of cellular signaling. The great affinity this polyether for its targets together with high specificity to inhibit certain protein phosphatases enables differential these proteins. Crystallographic structures complex OA show a 1:1 toxin ratio. Nevertheless, it found that is able self-associate under conditions although very little known about importance phenomenon. Here we review available knowledge on latter topic...
Background/Aims: Orthotopic liver transplantation (OLT) is the recommended treatment for patients at early stages of hepatocarcinoma (HCC) with portal hypertension and/or increased bilirubinemia, but without vascular-associated diseases.Tumor recurrence, which main drawback survival submitted to OLT HCC, has been related tumor-related variables and immunosuppressive therapies.We have previously shown that Tacrolimus (FK506) exerts a more potent pro-apoptotic anti-proliferative effects than...
Hepatocellular carcinoma (HCC) is the sixth most common neoplasia and fourth cause of cancer-related mortality worldwide. Sorafenib first-line molecular therapy for patients in an advanced stage HCC. However, recommended clinical dose associated with several complications, which derive from its lack cell specificity very low water solubility. To circumvent these drawbacks, present study we developed two sugar-coated polydiacetylene-based nanomicelles-Sorafenib carriers targeting mannose...
Highly specialized cells, the thyrocytes, express a thyroid-specific set of genes for thyroglobulin (Tg), thyroperoxidase, and transcription factors TTF-1, TTF-2, Pax-8. The implication small GTPase RhoA in TSH-mediated proliferation FRTL-5 rat thyroid cells has been previously demonstrated. To further analyze function cell differentiation patterns, we combined transient stable transfection assays to different mutant forms cells. Constitutively active (FRTL-5-RhoA QL cells) exhibited...
Hepatocellular carcinoma (HCC) is the most common subtype of primary liver cancer with varied incidence and epidemiology worldwide. Sorafenib still a recommended treatment for large proportion patients advanced HCC. Different patterns responsiveness have been identified in differentiated hepatoblastoma HepG2 cells metastatic HCC SNU449 cells. To define long non-codingRNA-microRNA-mRNA (lncRNA-miRNA-mRNA) predicted signatures related to selected hallmarks (apoptosis, autophagy, cell stress,...
Circulating tumor cells (CTCs) enumeration is a promising technique to predict cancer prognosis and treatment response. CTCs were evaluated in healthy subjects, cirrhotic controls hepatocarcinoma (HCC) patients. isolated using microfluidic system based on the expression of EpCAM, EGFR three epithelial mesenchymal transition (EMT) markers. Patients stratified according disease progression exitus. Although counts individual CTCs, clustered α-fetoprotein (AFP) at basal level patients...