A5048784517- Cancer Cells and Metastasis
- Gene expression and cancer classification
- Single-cell and spatial transcriptomics
- Adipose Tissue and Metabolism
- Cancer Genomics and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Muscle Physiology and Disorders
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Cell Image Analysis Techniques
- Adipokines, Inflammation, and Metabolic Diseases
- Computational Drug Discovery Methods
- Cancer, Lipids, and Metabolism
- Mesenchymal stem cell research
- Kruppel-like factors research
- Tissue Engineering and Regenerative Medicine
- Monoclonal and Polyclonal Antibodies Research
- Advanced Breast Cancer Therapies
- Cancer, Stress, Anesthesia, and Immune Response
- Histone Deacetylase Inhibitors Research
- Health Systems, Economic Evaluations, Quality of Life
- Nutrition and Health in Aging
- Advances in Cucurbitaceae Research
- Epigenetics and DNA Methylation
- Retinoids in leukemia and cellular processes
Indiana University – Purdue University Indianapolis
2014-2023
Indiana University School of Medicine
2013-2023
Indiana University Indianapolis
2020
University of Indianapolis
2020
Indianapolis Zoo
2020
Washington University in St. Louis
2019
University of Arkansas at Little Rock
2017
Richard L. Roudebush VA Medical Center
2017
Indiana University
2014
The University of Texas at San Antonio
2012
Preclinical studies of primary cancer cells are typically done after tumors removed from patients or animals at ambient atmospheric oxygen (O
Abstract Circulating microRNAs (miRNA) are emerging as important biomarkers of various diseases, including cancer. Intriguingly, circulating levels several miRNAs lower in patients with cancer compared healthy individuals. In this study, we tested the hypothesis that a miRNA might serve surrogate effects on expression or release distant organs. Here report muscle-enriched miR486 is breast individuals and difference replicated faithfully MMTV-PyMT MMTV-Her2 transgenic mouse models...
Abstract The transcription factor nuclear factor-kappaB (NF- κ B) is constitutively active in several cancers and a target of therapeutic development. We recently developed dimethylaminoparthenolide (DMAPT), clinical grade water-soluble analog parthenolide, as potent inhibitor NF- B demonstrated vitro vivo anti-tumor activities multiple cancers. In this study, we show DMAPT an epigenetic modulator functioning B-dependent -independent manner. DMAPT-mediated inhibition resulted elevated...
Abstract Recent reports of widespread genetic variation affecting regulation gene expression raise the possibility significant inter-individual differences in stem-progenitor-mature cell hierarchy adult organs. This has not been explored because paucity methods to quantitatively assess subpopulation normal epithelial cells on individual basis. We report remarkable differentiation capabilities as documented by phenotypic heterogeneity breast. Ethnicity and predisposition are partly...
Abstract Cell-type origin is one of the factors that determine molecular features tumors, but resources to validate this concept are scarce because technical difficulties in propagating major cell types adult organs. Previous attempts generate such study breast cancer have yielded predominantly basal-type lines. We created a panel immortalized lines from core biopsies ancestry-mapped healthy women form ductal structures similar normal 3D cultures and expressed markers types, including...
We hypothesized that quantitative MS / ‐based proteomics at multiple time points, incorporating immunoenrichment prior to rapid microwave and magnetic ( IM 2 ) sample preparation, might enable correlation of the relative expression CD 47 other low abundance proteins disease progression in experimental autoimmune encephalomyelitis EAE animal model sclerosis. To test our hypothesis, anti‐ antibodies were used enrich for . Decoding protein each point, with 47‐immunoenriched samples targeted...
Breast cancer progression is associated with systemic effects, including functional limitations and sarcopenia without the appearance of overt cachexia. Autocrine/paracrine actions cytokines/chemokines produced by cells mediate limitations. The cytokine-inducible transcription factor NF-κB could be central to this process, as it displays oncogenic functions integral Pax7:MyoD:Pgc-1β:miR-486 myogenesis axis. We tested possibility using MMTV-PyMT transgenic mammary tumor model inhibitor...
We hypothesized that quantitative MS/MS-based proteomics at multiple time points, incorporating rapid microwave and magnetic (M(2) ) sample preparation, could enable relative protein expression to be correlated disease progression in the experimental autoimmune encephalomyelitis (EAE) animal model of sclerosis. To test our hypothesis, microwave-assisted reduction/alkylation/digestion proteins from brain tissue lysates bound C8 beads isobaric chemical labeling were performed released...
There is a need to individualize assays for tumor molecular phenotyping, given variations in the differentiation status of and normal tissues different patients. To address this, we performed single-cell genomics breast tumors adjacent cells propagated short duration under growth conditions that enable epithelial reprogramming. Cells analyzed were either unselected specific subpopulation or phenotypically defined as undifferentiated highly clonogenic ALDH+/CD49f+/EpCAM+ luminal progenitors,...
Functional modeling of normal breast epithelial hierarchy and stromal-epithelial cell interactions have been difficult due to inability obtain sufficient stem-progenitor-mature stromal cells. Recently reported reprogramming assay has partially overcome this limitation, but cross-contamination cells from the feeder layer is a concern. The purpose study was develop feeder-layer-independent inexpensive method propagate multiple types limited tissue resources. Cells obtained after enzymatic...
Abstract Adipose-derived stem cells (ASC) have received considerable attention in oncology because of the known direct link between obesity and cancer as well use ASCs reconstructive surgery after tumor ablation. Previous studies documented how commandeer to support their survival by altering extracellular matrix composition stiffness, migration, metastasis. This study focused on delineating effects adipocytes self-renewal stem/progenitor hierarchy breast epithelial cells. The immortalized...
Cancer cells develop tactics to circumvent the interventions by desensitizing themselves interventions. Amongst many, principle routes of desensitization include a) activation survival pathways (e.g. NF-kB, PARP) and b) downregulation cell death CD95/CD95L). As a result, it requires high therapeutic dose achieve tumor regression which, in turn damages normal through collateral effects. Methods are needed sensitize low non-responsive resistant including cancer stem (CSCs) order evoke better...
Abstract The incidence of/mortality from the most aggressive triple negative breast cancer (TNBC) is higher in African American- (AA) than Caucasian (CA)-women. In contrast, Hispanic-women generally less aggressive. There an ongoing debate as to whether AA-women have increased of TNBCs that poor outcome or there are unique biological factors promote biology. Through approaches, we provide evidence for distinct biology normal breasts CA-women, AA-women, and Hispanic-women. Using resources...
of Horticultural Research, Hessarghatta, Bengaluru, Karnataka to study the effect fertigation with different levels NPK on growth, fruit yield and quality hybrid ridge gourd [Luffa acutangula (L.) Roxb] Arka Vikram.The experiment was laid out eight treatments in randomized complete block design replicated thrice.The results revealed that treatment T 3 i.e application water soluble fertilizers @ 150:90:150 kg ha -1 through recorded maximum vine length (635.31cm),internodal (14.52 cm), number...
Abstract Autologous adipose stem cell transplantation is increasingly being used for soft tissue reconstruction, particularly after mastectomy. However, a recent study demonstrated recurrence of invasive ductal carcinoma in the breast within four months autologous fat grafting. Previous studies have documented how cancer cells commandeer (ASC) to support their survival by altering extracellular matrix composition and stiffness. This focused on delineating effects ASCs self-renewal capacity...
Abstract Breast cancer is the leading cause of cancer-related death in non-smoking women United States. Despite tremendous amounts information about etiology breast cancer, many questions remain unanswered. It has been demonstrated that specific subtypes differ their sensitivity to chemotherapeutic and targeted therapies. The oncogenic events signaling pathways driving these tumor are distinct. This indicates increased knowledge molecular defining unique will provide opportunities for novel...