A5070803103- Neurofibromatosis and Schwannoma Cases
- Neuroblastoma Research and Treatments
- Nerve injury and regeneration
- HER2/EGFR in Cancer Research
- Cancer Genomics and Diagnostics
- Monoclonal and Polyclonal Antibodies Research
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Lung Cancer Treatments and Mutations
- Management of metastatic bone disease
- Atherosclerosis and Cardiovascular Diseases
- RNA modifications and cancer
- BRCA gene mutations in cancer
- Cancer Research and Treatments
- Cancer-related Molecular Pathways
- Angiogenesis and VEGF in Cancer
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Chronic Kidney Disease and Diabetes
- Breast Cancer Treatment Studies
- Hemoglobinopathies and Related Disorders
- DNA Repair Mechanisms
- Erythrocyte Function and Pathophysiology
- Cytokine Signaling Pathways and Interactions
- Advanced Biosensing Techniques and Applications
Indiana University – Purdue University Indianapolis
2011-2023
Indiana University School of Medicine
2008-2023
University of Indianapolis
2016-2023
Indiana University
2007-2021
University School
2021
Pediatrics and Genetics
2006
Center for Vascular Biology Research
2004
Preclinical studies of primary cancer cells are typically done after tumors removed from patients or animals at ambient atmospheric oxygen (O
Decreased renal blood flow following an ischemic insult contributes to a reduction in glomerular filtration. However, little is known about the underlying cellular or subcellular mechanisms mediating reduced human acute kidney injury (AKI) failure (ARF). To examine vascular ischemia, intraoperative graft biopsies were performed after reperfusion 21 cadaveric allografts. Confocal fluorescence microscopy was utilized smooth muscle and endothelial cell integrity as well peritubular interstitial...
In Brief Objective: To evaluate the effects of surgical weight loss on hepatic lipid peroxidation levels and cytochrome P-450 protein expression in patients with nonalcoholic fatty liver disease (NAFLD). Summary Background Data: NAFLD steatohepatitis (NASH) affect (CYP) activity, CYP2E1 may play a role pathogenesis NASH through induction oxidative stress peroxidation. are associated increased systemic elevated but CYP3A4/5 activity is decreased. Methods: Liver biopsies from 20 who underwent...
// Lakshmi Prabhu 1,* , Rasika Mundade Benlian Wang 2 Han Wei 1 Antja-Voy Hartley Matthew Martin Kyle McElyea 3 Constance J. Temm George Sandusky Yunlong Liu 4,5 and Tao Lu 1,4,6 Department of Pharmacology Toxicology, Indiana University School Medicine, Indianapolis, IN, USA Center for Proteomics Bioinformatics, Case Western Reserve University, Cleveland, Ohio, Pathology Laboratory 4 Medical Molecular Genetics, Research Library Building, 5 Computational Biology Health Information...
Family with sequence similarity 83 member A (FAM83A) presents oncogenic properties in several cancers including breast cancer. Recently, we reported FAM83A overexpression normal tissues from women at high risk of We now hypothesize that is a key factor cancer initiation.Immunohistochemical staining was used to evaluate protein levels both tissue microarray (TMA, N = 411) and tumor TMA (N 349). EGFR its correlation expression were also assessed. Lentivirus-mediated manipulation primary...
Study of genomic aberrations leading to immortalization epithelial cells has been technically challenging due the lack isogenic models. To address this, we used healthy primary breast luminal different genetic ancestry and their hTERT-immortalized counterparts identify transcriptomic changes associated with immortalization. Elevated expression TONSL (Tonsoku-like, DNA repair protein) was identified as one earliest events during TONSL, which is located on chromosome 8q24.3, found be amplified...
The early nephropathy in obese, diabetic, dyslipidemic (ZS) rats is characterized by tubular lipid accumulation and pervasive inflammation, two critically interrelated events. We now tested the hypothesis that proximal tubules from ZS obese diabetic vivo, tubule cells (NRK52E) exposed to oxidized LDL (oxLDL) vitro, change their normally quiescent epithelial phenotype into a proinflammatory phenotype. Urine of contained more peroxides, LOX-1, membrane receptor internalizes lipids, was...
Coronary transient receptor potential canonical (TRPC) channel expression is elevated in metabolic syndrome (MetS). However, differential contribution of TRPCs to coronary pathology MetS not fully elucidated. We investigated the roles TRPC1 and TRPC6 isoforms arteries pigs determined whether long-term treatment with a mineralocorticoid inhibitor, spironolactone, attenuates TRPC associated dysfunctions. exhibited significant atherosclerosis, endothelial dysfunction, increased...
Abstract Background Genome-wide association studies have identified several breast cancer susceptibility loci. However, biomarkers for risk assessment are still missing. Here, we investigated cancer-related molecular changes detected in tissues from women at high prior to disease manifestation. Disease-free tissue cores donated by healthy ( N = 146, median age 39 years) were processed both methylome (MethylCap) and transcriptome (Illumina’s HiSeq4000) sequencing. Analysis of microarray...
For determining the effects of anandamide (ANA) on renal hemodynamics and microcirculation, a clearance study was performed in Sprague-Dawley rats that received injections ANA doses 15, 150, 1500 pmol/kg. At up to 150 pmol/g, significantly decreased GFR increased blood flow (RBF) without affecting mean arterial pressure (MAP). In presence cannabinoid type 1 (CB1) receptor antagonist AM251, only 15-pmol/kg dose RBF altering MAP, with higher having no effect GFR, RBF, or MAP. By contrast,...
LOX-1 is a multifunctional membrane receptor that binds and internalizes oxidized LDL (oxLDL). We tested the hypothesis blockade of with an anti-LOX-1 antibody limits nephropathy in male rats diabetes dyslipidemia (ZS rats; F(1) hybrid product Zucker fatty diabetic spontaneous hypertensive heart failure rats). Lean ZS were controls, while untreated obese (OM), injected nonspecific rabbit IgG (OM-IgG; 2 microg intravenous injection given weekly), (OM-Ab; weekly) experimental groups. The...
Anomalous inflammatory responses triggered by the metabolic syndrome cause renal injury. This discovery links lipid accumulation with lipotoxicity to inflammation and may explain insidious fibrosis cellular decay characteristic of nephropathy in syndrome. However, it is not clear whether control protects kidney independently accumulation, which a required step for hyperglycemia dyslipidemia. We hypothesized that rats syndrome, overt nephropathy, treatment mycophenolate mofetil (MMF; 10...
The biologic basis of genetic ancestry-dependent variability in disease incidence and outcome is just beginning to be explored. We recently reported enrichment a population ZEB1-expressing cells located adjacent ductal epithelial normal breasts women African ancestry compared those European ancestry. In this study, we demonstrate that these have properties fibroadipogenic/mesenchymal stromal express PROCR PDGFRα transdifferentiate into adipogenic osteogenic lineages. + /ZEB1 /PDGFRα+ (PZP)...
Abstract Purpose: Plexiform neurofibromas (PNF) are benign peripheral nerve sheath tumors (PNST) associated with neurofibromatosis type 1 (NF1). Despite similar histologic appearance, these neoplasms exhibit diverse evolutionary trajectories, a subset progressing to malignant tumor (MPNST), the leading cause of premature death in individuals NF1. Malignant transformation PNF often occurs through development atypical neurofibroma (ANF) precursor lesions characterized by distinct...
Recombinant adeno-associated virus (rAAV)-mediated gene delivery shows promise to transduce the pancreas, but safety/efficacy in a neoplastic context is not well established. To identify an ideal AAV serotype, route, and vector dose assess safety, we have investigated use of three serotypes (6, 8, 9) expressing GFP self-complementary (sc) under EF1α promoter (scAAV.GFP) following systemic or retrograde pancreatic intraductal delivery. Systemic scAAV9.GFP transduced pancreas with high...
Angiogenesis is essential for prostate cancer development and metastasis. Antiangiogenic therapy targeting tumor neovasculature, therefore, represents a promising approach treatment. We hypothesized that adenoviral-mediated delivery of combination antiangiogenic factors might have an enhanced antitumor response. developed the adenoviral vectors Ad-hEndo-angio, expressing unique, chimeric human endostatin-angiostatin fusion protein, Ad-sTie2, soluble form endothelium-specific receptor...
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Generalized capillary dysfunction is a morbid element in the metabolic syndrome, and it likely involved its complications. We tested hypothesis that vast amounts of serum albumin previously observed kidneys rats with syndrome were caused, part, by leakage from renal peritubular capillaries. report herein large scale leaks plasma fluid capillaries syndrome. This finding was directly demonstrated vivo, presence leftover residue confirmed leak postmortem kidney specimens. Moreover, interstitial...
<div>AbstractPurpose:<p>Plexiform neurofibromas (PNF) are benign peripheral nerve sheath tumors (PNST) associated with neurofibromatosis type 1 (NF1). Despite similar histologic appearance, these neoplasms exhibit diverse evolutionary trajectories, a subset progressing to malignant tumor (MPNST), the leading cause of premature death in individuals NF1. Malignant transformation PNF often occurs through development atypical neurofibroma (ANF) precursor lesions characterized by...
We describe a child with dyserythropoietic anemia, thrombocytosis, functional platelet defect, and megakaryocyte dysplasia. show that (i) this constellation of hematopoietic abnormalities was due to germline mutation within the 5′ untranslated region (5′UTR) globin transcription factor 1 (GATA1); (ii) impaired 5′UTR GATA1 splicing site, promoted production shortened isoform lacking N-terminus; (iii) expression N-terminus is restricted erythroblasts megakaryocytes in normal marrow, consistent...