A5077837457- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- BRCA gene mutations in cancer
- Estrogen and related hormone effects
- Gene expression and cancer classification
- Ubiquitin and proteasome pathways
- NF-κB Signaling Pathways
- Muscle Physiology and Disorders
- Advanced Breast Cancer Therapies
- Cytokine Signaling Pathways and Interactions
- Single-cell and spatial transcriptomics
- Cancer-related Molecular Pathways
- PI3K/AKT/mTOR signaling in cancer
- HER2/EGFR in Cancer Research
- Retinoids in leukemia and cellular processes
- Molecular Biology Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- MicroRNA in disease regulation
- Nutrition, Genetics, and Disease
- Analytical Chemistry and Sensors
- Epigenetics and DNA Methylation
- Blood properties and coagulation
- Cancer-related molecular mechanisms research
- DNA Repair Mechanisms
Indiana University – Purdue University Indianapolis
2016-2025
Richard L. Roudebush VA Medical Center
2016-2025
Indiana University School of Medicine
2016-2025
Indiana University Health
2015-2025
University of Indianapolis
2004-2024
Indiana University
2014-2024
Indiana University Melvin and Bren Simon Comprehensive Cancer Center
2001-2023
University School
2015-2023
Cancer Research Center
2017-2022
Government of the United States of America
2020-2022
Abstract Introduction A subpopulation (CD44 + /CD24 - ) of breast cancer cells has been reported to have stem/progenitor cell properties. The aim this study was investigate whether the unique ability invade, home, and proliferate at sites metastasis. Methods CD44 CD24 expression determined by flow cytometry. Northern blotting used determine proinvasive 'bone lung metastasis signature' genes. matrigel invasion assay intracardiac inoculation into nude mice were evaluate invasion, homing...
Estrogen receptors (ERs) mediate most of the biological effects estrogen in mammary and uterine epithelial cells by binding to response elements promoter region target genes or through protein-protein interactions. Anti-estrogens such as tamoxifen inhibit growth ER-positive breast cancers reducing expression estrogen-regulated genes. However, anti-estrogen-resistant tumors remains a significant clinical problem. Here we show that phosphatidylinositol (PI) 3-kinase AKT activate ERalpha...
Breast cancers often progress from a hormone-dependent, nonmetastatic, antiestrogen-sensitive phenotype to hormone-independent, antiestrogen- and chemotherapy-resistant with highly invasive metastatic growth properties. This progression is usually accompanied by altered function of the estrogen receptor (ER) or outgrowth ER-negative cancer cells. To understand molecular mechanisms responsible for breast cancers, activities transcription factor NF-kappaB (which modulates expression genes...
Metastasis of cancer cells is a complex process involving multiple steps including invasion, angiogenesis, and trafficking through blood vessels, extravasations, organ-specific homing, growth. While matrix metalloproteinases, urokinase-type plasminogen activator, cytokines play major role in invasion chemokines such as stromal derived factor-1α (SDF-1α) their receptors CXCR4 are thought to critical motility, proliferation at specific metastatic sites. We others have previously reported that...
We recently published PROGgene, a tool that can be used to study prognostic implications of genes in various cancers. The first version the had several areas for improvement. In this paper we present some major enhancements have made on existing new version, PROGgeneV2. PROGgeneV2, modifications enhance survival analysis capability tool. First, increased repository public studies catalogued our by almost two folds. also added additional functionalities perform variety ways. Survival now...
Estradiol (E2) regulates gene expression at the transcriptional level by functioning as a ligand for estrogen receptor alpha (ERα) and beta (ERβ). E2-inducible proteins c-Myc E2Fs are required optimal ERα activity secondary responses, respectively. We show that E2 induces 21 microRNAs represses seven in MCF-7 breast cancer cells; these have potential to control 420 E2-regulated 757 non-E2-regulated mRNAs post-transcriptional level. The serine/threonine kinase, AKT, alters of microRNAs....
Abstract A novel secreted cytokine, termed IL-17F, was cloned using nested RACE PCR. This cytokine bears homology to IL-17. IL-17F expressed only in activated CD4+ T cells and monocytes. Recombinant human did not stimulate the proliferation of hematopoietic progenitors or migration mature leukocytes. However, it markedly inhibited angiogenesis endothelial induced produce IL-2, TGF-β, monocyte chemoattractant protein-1.
Abstract Purpose: FOXA1, a forkhead family transcription factor, is essential for optimum expression of ∼50% estrogen receptor α (ERα):estrogen responsive genes. FOXA1 expressed in breast cancer cells. It segregates with genes that characterize the luminal subtypes DNA microarray analyses. The utility as possible independent prognostic factor has not been determined cancers. Materials and Methods: A tissue comprising tumors from 438 patients 15.4 years median follow-up was analyzed by...
We have characterized the promoter of mouse retinoic acid receptor alpha 2 (mRAR-alpha 2) isoform. This contains a response element (RARE) that closely resembles RARE is present in RAR-beta promoter. Moreover, RAR-alpha and proximal sequences are similar to each other generate transcripts whose respective start sites located at positions. The consists two directly repeated 5'-GTTCA-3' motifs which all three RARs (alpha, beta, gamma) bind vitro.
Breast cancer cells with CD44+/CD24- cell surface marker expression profile are proposed as stem (CSCs). Normal breast epithelial that express higher levels of stem/progenitor associated genes. We, amongst others, have shown undergone to mesenchymal transition (EMT) display the phenotype. However, whether all genes induce EMT confer phenotype is unknown. We hypothesized only a subset generates cells. MCF-10A cells, subpopulation which spontaneously acquire phenotype, were used identify...
Gold nanorod molecular probes (GNrMPs) were designed and fabricated for multiplex identification of cell surface markers in HBECs. Cells probed directly using dark field microscopy integrated with a spectral imager simultaneous detection up to three markers. The immunophenotype composition these lines indicative their metastasis potential was assessed the GNrMPs. technique has become an important tool diagnosis prognosis breast other cancers.
As systemic cancer therapies improve and are able to control metastatic disease outside the central nervous system, brain is increasingly first site of relapse. The blood-brain barrier (BBB) represents a major challenge delivery therapeutics brain. Macrophages originating from circulating monocytes infiltrate metastases while BBB intact. Here, we show that this ability can be exploited deliver both diagnostic therapeutic nanoparticles specifically experimental breast cancer.
Identification of prognostic mRNA biomarkers has been done for various cancer types. The data that are published from such studies archived in public repositories. There hundreds datasets available multiple types Wealth can be utilized to study implications different cancers as well populations or subtypes same cancer.We have created a web application used studying variety cancers. We compiled repositories GEO, EBI Array Express and Cancer Genome Atlas creating this tool. With 64 patient...
Although the incidence of breast cancer metastasis (BCM) in brain has increased significantly triple-negative (TNBC), mechanisms remain elusive. Using vivo mouse models for BCM brain, we observed that TNBC cells crossed blood–brain barrier (BBB), lodged microvasculature and remained adjacent to microvascular endothelial (BMECs). Breaching BBB by TNBCs resulted permeability changes ZO-1 claudin-5 tight junction (TJ) protein structures. Angiopoietin-2 expression was elevated BMECs correlated...