A5100452661- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA modifications and cancer
- RNA Research and Splicing
- Atrial Fibrillation Management and Outcomes
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Ferroptosis and cancer prognosis
- NF-κB Signaling Pathways
- Peptidase Inhibition and Analysis
- Genomics and Phylogenetic Studies
- Genomics, phytochemicals, and oxidative stress
- Cardiac electrophysiology and arrhythmias
- Heat shock proteins research
- Single-cell and spatial transcriptomics
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Electrochemical sensors and biosensors
- Histone Deacetylase Inhibitors Research
- Immune Cell Function and Interaction
- Acute Myeloid Leukemia Research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Ion channel regulation and function
- Bacteriophages and microbial interactions
First Affiliated Hospital of Harbin Medical University
2011-2025
Xi'an Jiaotong University
2006-2025
Harbin Medical University
2011-2025
Hefei Institutes of Physical Science
2022-2025
Chinese Academy of Sciences
2022-2025
Qingdao Municipal Hospital
2025
Shanxi Medical University
2024
Second Hospital of Shanxi Medical University
2024
Polar Research Institute of China
2024
Ministry of Natural Resources
2024
The ubiquitous inducible transcription factor NF-κB plays central roles in immune and inflammatory responses tumorigenesis. Complex posttranslational modifications of the p65 subunit (RelA) are a major aspect extremely flexible regulation activity. Although phosphorylation, acetylation, ubiquitination, lysine methylation have been well described, arginine has not yet found. We now report that, response to IL-1β, is dimethylated on 30 (R30) by protein-arginine methyltransferase 5 (PRMT5)....
Abstract Mutations in amino acid sequences can provoke changes protein function. Accurate and unsupervised prediction of mutation effects is critical biotechnology biomedicine, but remains a fundamental challenge. To resolve this challenge, here we present Pro tein M utational E ffect P redictor (ProMEP), general multiple sequence alignment-free method that enables zero-shot effects. A multimodal deep representation learning model embedded ProMEP was developed to comprehensively learn both...
Dynamic changes in DNA methylation and demethylation reprogram transcriptional outputs to instruct lineage specification during development. Here, we applied an integrative epigenomic approach unveil (hydroxy)methylation dynamics representing major endodermal intermediates pancreatic differentiation of human embryonic stem cells (hESCs). We found that 5-hydroxymethylcytosine (5hmC) marks genomic regions be demethylated the descendent lineage, thus reshaping landscapes progression....
Tet-mediated DNA demethylation plays an important role in shaping the epigenetic landscape and chromatin accessibility to control gene expression. While several studies demonstrated pivotal roles of Tet regulating embryonic development, little is known about their functions heart development. Here we analyze methylation hydroxymethylation dynamics during early cardiac development both human mice. We find that cardiac-specific deletion Tet2 Tet3 mice (Tet2/3-DKO) leads ventricular...
Y box protein 1 (YBX1) is a well known oncoprotein that has tumor-promoting functions. YBX1 widely considered to be an attractive therapeutic target in cancer. To develop novel therapeutics YBX1, it of great importance understand how finely regulated Previously, we have shown could function as tumor promoter through phosphorylation its Ser-165 residue, leading the activation NF-κB signaling pathway (1). In this study, using mass spectrometry analysis, discovered distinct site, Ser-176, on...
// Lakshmi Prabhu 1,* , Rasika Mundade Benlian Wang 2 Han Wei 1 Antja-Voy Hartley Matthew Martin Kyle McElyea 3 Constance J. Temm George Sandusky Yunlong Liu 4,5 and Tao Lu 1,4,6 Department of Pharmacology Toxicology, Indiana University School Medicine, Indianapolis, IN, USA Center for Proteomics Bioinformatics, Case Western Reserve University, Cleveland, Ohio, Pathology Laboratory 4 Medical Molecular Genetics, Research Library Building, 5 Computational Biology Health Information...
Abstract The multifunctional protein Y-box binding 1 (YBX1), is a critical regulator of transcription and translation, widely recognized as an oncogenic driver in several solid tumors, including colorectal cancer (CRC). However, very little known about the upstream or downstream factors that underlie YBX1′s regulation involvement CRC. Previously, we demonstrated YBX1 overexpression correlated with potent activation nuclear factor κB (NF-κB), well-known believed to be crucial CRC progression....
Abstract Inactivation of tumor-infiltrating lymphocytes (TIL) is one the mechanisms mitigating antitumor immunity during tumor onset and progression. Epigenetic abnormalities are regarded as a major culprit contributing to dysfunction TILs within microenvironments. In this study, we used murine model melanoma discover that Tet2 inactivation significantly enhances activity with an efficacy comparable immune checkpoint inhibition imposed by anti-PD-L1 treatment. Single-cell RNA-sequencing...
// Lakshmi Prabhu 1 , Han Wei Lan Chen 2,3,4 Özlem Demir 5 George Sandusky 6 Emily Sun John Wang Jessica Mo Lifan Zeng 2,3 Melissa Fishel 9 Ahmad Safa Rommie Amaro Murray Korc 7 Zhong-Yin Zhang 3,4 and Tao Lu 1,3,8 Department of Pharmacology Toxicology, Indiana University School Medicine, Indianapolis, IN, USA 2 Chemical Genomics Core Facility, 3 Biochemistry Molecular Biology, 4 Medicinal Chemistry Pharmacology, Purdue University, West Lafayette, Biochemistry, California, San Diego, La...
The progressive loss of dopaminergic neurons and abnormal iron deposition in the central nervous system (CNS) are key pathogenic mechanisms Parkinson's disease (PD). This study aimed to explore relationship between specific CNS regions striatal dysfunction using 18F-AV133 PET/MR imaging. Based on Hoehn-Yahr stage, 24 patients with early-stage PD (EPD, stage ≤ 2.5), 17 late-stage (LPD, ≥ 3), 30 healthy controls (HCs) were recruited for scale evaluation. uptake ratio (SUR) subregions was...
The proposed workflow of the drug development pipeline with adapted AlphaLISA HTS-based screening to discover inhibitors epigenetic targets like PRMT5 possible significance in disease treatment.
The overexpression of PRMT5 is highly correlated to poor clinical outcomes for colorectal cancer (CRC) patients. Importantly, our previous work demonstrated that could substantially augment activation the nuclear factor kappa B (NF-κB) via methylation arginine 30 (R30) on its p65 subunit, while knockdown showed opposite effect. However, precise mechanisms governing this PRMT5/NF-κB axis are still largely unknown. Here, we report a novel finding phosphorylated serine 15 (S15) in response...
The dysfunction and apoptosis of vascular endothelial cells are the initiating links in formation atherosclerosis. N6-methyladenosine (m6A) is an extremely extensive RNA methylation modification its abnormality leads to occurrence various human diseases. In this study, we explored effects demethylase α-ketoglutarate-dependent dioxygenase ALKB homolog 5 (ALKBH5) on TNF-α-induced umbilical vein (HUVECs). TNF-α-treated HUVECs, expression ALKBH5 was significantly decreased. overexpression...
Ubiquitination is a reversible process that not only controls protein synthesis and degradation, but also essential for transport, localization biological activity. Deubiquitinating enzyme (DUB) dysfunction leads to various diseases, including cancer. In this study, we aimed explore the functions mechanisms of crucial DUBs in head neck squamous cell carcinoma (HNSCC). Based on bioinformatic analysis immunohistochemistry detection, YOD1 was identified be significantly downregulated HNSCC...