A5062018268- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- RNA and protein synthesis mechanisms
- Cancer Research and Treatments
- Bacterial Infections and Vaccines
- Evolution and Genetic Dynamics
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Antimicrobial Resistance in Staphylococcus
- PARP inhibition in cancer therapy
- Machine Learning in Bioinformatics
- Immune cells in cancer
- interferon and immune responses
- Bacterial Identification and Susceptibility Testing
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Genomics and Phylogenetic Studies
- Yersinia bacterium, plague, ectoparasites research
- Trace Elements in Health
- Antimicrobial Peptides and Activities
- Drug Transport and Resistance Mechanisms
Harvard University
2020-2024
Imperial College London
2013-2022
Medical Research Council
2014-2021
Délégation Paris 5
2006-2010
Université Paris Cité
2006-2010
Inserm
2004-2010
Many bacterial pathogens cause persistent infections despite repeated antibiotic exposure. Bacterial persisters are antibiotic-tolerant cells, but little is known about their growth status and the signals pathways leading to formation in infected tissues. We used fluorescent single-cell analysis identify Salmonella during infection. These were part of a nonreplicating population formed immediately after uptake by macrophages induced vacuolar acidification nutritional deprivation, conditions...
Actively persistent Salmonella A proportion of cells can enter a reversible state growth arrest, which allows them to tolerate environmental stress such as antibiotics. Stapels et al. found that these are not dormant but actively modulating their environment. within host macrophage niche deployed specialized type 3 secretory system called SPI-2 deliver virulence factors, including SteE, into cells. SteE changed the cytokine profile infected macrophages reprogram noninflammatory and...
Several important pathogens cause disease by surviving and replicating within host cells. Bacterial proliferation is the product of both replication killing undergone population. However, these processes are difficult to distinguish, usually assessed together determination net bacterial load. In addition, measurement load does not reveal heterogeneity pathogen populations. This particularly in persistent infections which slow or nongrowing bacteria thought have a major impact. Here we report...
Summary Although type IV pili (Tfp) are among the commonest virulence factors in bacteria, their biogenesis by complex machineries of 12–15 proteins, and thereby function remains poorly understood. Interestingly, some these proteins were reported to merely antagonize retraction fibres powered PilT, because piliation could be restored absence a mutation pilT gene. The recent identification 15 Pil dedicated Tfp Neisseria meningitidis offered us unprecedented possibility define exact...
ABSTRACT Salmonella enterica serovars cause severe diseases in humans, such as gastroenteritis and typhoid fever. The development of systemic disease is dependent on a type III secretion system (T3SS) encoded by pathogenicity island-2 (SPI-2). Translocation effector proteins across the -containing vacuole, via SPI-2 T3SS, enables bacterial replication within host cells, including macrophages. Here, we investigated contribution these effectors to intramacrophage serovar Typhimurium using...
Many Gram-negative bacterial pathogens antagonize anti-bacterial immunity through translocated effector proteins that inhibit pro-inflammatory signaling. In addition, the intracellular pathogen Salmonella enterica serovar Typhimurium initiates an anti-inflammatory transcriptional response in macrophages its protein SteE. However, target(s) and molecular mechanism of SteE remain unknown. Here, we demonstrate converts both amino acid substrate specificity host pleiotropic serine/threonine...
Non-typhoidal Salmonella strains are responsible for invasive infections associated with high mortality and recurrence in sub-Saharan Africa, there is strong evidence clonal relapse following antibiotic treatment. Persisters non-growing bacteria that thought to be the recalcitrance of many antibiotics. Toxin-antitoxin systems stress-responsive elements important persister formation, specifically during infection. Here, we report analysis formation clinical Typhimurium Enteritidis human...
Summary The attachment of pathogenic Neisseria species to human cells, in which type IV pili (Tfp) play a key but incompletely defined role, depends on the ability these bacteria establish contacts with target cells also interbacterial interactions. In an effort improve our understanding molecular mechanisms N. meningitidis adherence we screened collection mutants for those presenting reduced cell line. Besides underscoring central role Tfp this process, analysis led identification...
Summary Type IV pili (Tfp) play a critical role in the pathogenic lifestyle of Neisseria meningitidis and N. gonorrhoeae , notably by facilitating bacterial attachment to human cells, but our understanding their biogenesis, during which fibres are assembled periplasm, then emerge onto cell surface stabilized, remains fragmentary. We therefore sought identify genes required for Tfp formation screening genome‐wide collection mutants those that were unable form aggregates, another phenotype...
Type IV pili (Tfp) are widespread filamentous bacterial organelles that mediate multiple virulence-related phenotypes. They composed mainly of pilin subunits, which processed before filament assembly by dedicated prepilin peptidases. Other proteins these peptidases, whose molecular nature and mode action remain enigmatic, play critical roles in Tfp biology. We have performed a detailed structure/function analysis one such protein, PilX from Neisseria meningitidis, is crucial for formation...
Mycobacterium tuberculosis infections result in a spectrum of clinical outcomes, and frequently the infection persists latent, clinically asymptomatic state. The within-host bacterial population is likely to be heterogeneous, it thought that persistent mycobacteria arise from small viable, but non-replicating (VBNR) cells. These are antibiotic tolerant necessitate prolonged treatment. Little known about these mycobacteria, since they very difficult isolate. To address this, we have...