A5040698114- Ion Channels and Receptors
- Bacterial Genetics and Biotechnology
- Nicotinic Acetylcholine Receptors Study
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Ion channel regulation and function
- Plant-based Medicinal Research
- Trace Elements in Health
- Machine Learning in Bioinformatics
- Toxin Mechanisms and Immunotoxins
- Receptor Mechanisms and Signaling
- Crystallization and Solubility Studies
- Neurobiology and Insect Physiology Research
- bioluminescence and chemiluminescence research
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Pain Mechanisms and Treatments
- Ubiquitin and proteasome pathways
- Neurological Disorders and Treatments
- Pharmacogenetics and Drug Metabolism
- Bioactive Compounds and Antitumor Agents
- Amino Acid Enzymes and Metabolism
- Antibiotic Resistance in Bacteria
- Metabolism and Genetic Disorders
KU Leuven
2017-2020
VIB-VUB Center for Structural Biology
2019
Vrije Universiteit Brussel
2019
University of Basel
2013-2015
Nicotinic acetylcholine receptors (nAChRs) belong to the family of pentameric ligand-gated ion channels and mediate fast excitatory transmission in central peripheral nervous systems. Among different existing receptor subtypes, homomeric α7 nAChR has attracted considerable attention because its possible implication several neurological psychiatric disorders, including cognitive decline associated with Alzheimer's disease or schizophrenia. Allosteric modulators are particular interest as...
Key points Mutagenesis at positively charged amino acids (arginines and lysines) (R1–R4) in the voltage‐sensor domain (transmembrane segment (S) 4) of voltage‐gated Na + , K Ca 2+ channels can lead to an alternative ion permeation pathway distinct from central pore. Recently, a non‐canonical was described TRPM3, member transient receptor potential (TRP) superfamily. The pore exists native TRPM3 channel be activated by co‐stimulation endogenous agonist pregnenolone sulphate antifungal drug...
Omp85 proteins mediate translocation of polypeptide substrates across and into cellular membranes. They share a common architecture comprising substrate-interacting POTRA domains, C-terminal 16-stranded β-barrel pore two signature motifs located on the inner barrel wall at tip extended L6 loop. The observation distinct conformations loop in available structures previously suggested functional role conformational changes mechanism. Here we present 2.5 Å resolution structure variant secretion...
TRPM3 channels play important roles in the detection of noxious heat and inflammatory thermal hyperalgesia. The activity these ion somatosensory neurons is tightly regulated by µ-opioid receptors through signaling Gβγ proteins, thereby reducing TRPM3-mediated pain. We show here that directly binds to a domain 10 amino acids solve cocrystal structure this together with Gβγ. Using data mutational analysis full-length we pinpoint three their interacting partners Gβ
Neonicotinoids, sometimes called ‘neonics’, are a class of insecticides targeting neuronal nicotinic acetylcholine receptors, which belong to the family pentameric ligand-gated ion channels (pLGICs) or Cys-loop receptors. The widespread application these neurotoxic in agriculture is tied worldwide decline bee populations, due their sublethal effects on memory, behavior and reproduction. In 2018, member states European Union, agreed ban most neonicotinoids, with exemption for greenhouses....
β-barrel proteins are key functional components of the outer membranes gram-negative bacteria, mitochondria and plastids. They mediate transport across membrane, act as receptors involved in bacterial pathogenicity. Despite their crucial roles, assembly membrane insertion proteins, which mediated by OMP85 family, have remained elusive. The crystal structure Escherichia coli protein TamA [1], is autotransporter biogenesis, now provides a novel perspective on assembly. was crystallized lipidic...
Page s139 cleavage complex formation and reversal is not fully understood for any type II topoisomerase.In order to further our understanding of the topo action, we have solved crystal structures representing sequential states in a DNA by topoisomerase IV from S. pneumoniae.A 3.1 Å resolution structure captured novel antibacterial dione represents drugarrested form intermediate reveals two drug molecules intercalated at symmetrically cleaved B-form gate stabilized drug-specific protein...